We narrowed to 8,219 results for: Lif

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Tol2-TLCV2

Plasmid

#196331

Purpose

the TLCV2 plasmid with Tol2 recombination sites for integration

Depositor

Itamar Harel

Article

Moses et al eLife. 2023 Oct 24;12:e85960. doi: 10.75

Insert

Cas9-2A-eGFP

Use

CRISPR; Fish expression

Expression

Bacterial, Mammalian, and Y…

Promoter

Tight TRE promoter

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pAM198

Plasmid

#227657

Purpose

pRS305 His-HRV3C-Rpn5 WT

Depositor

Andreas Martin

Article

Greene et al Elife. 2019 Nov 28;8:e49806. doi: 10.755

Insert

rpn5 (RPN5 Budding Yeast)

Tags

His-HRV3C-

Expression

Yeast

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM200

Plasmid

#227659

Purpose

pRS305 3XFLAG-HRV3C-Rpn5 WT

Depositor

Andreas Martin

Article

Greene et al Elife. 2019 Nov 28;8:e49806. doi: 10.755

Insert

rpn5 (RPN5 Budding Yeast)

Tags

3X FLAG-HRV3C-

Expression

Yeast

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM202

Plasmid

#227661

Purpose

pRS316 Rpn6 promoter-Rpn5 ORF-Rpn5 terminator

Depositor

Andreas Martin

Article

Greene et al Elife. 2019 Nov 28;8:e49806. doi: 10.755

Insert

rpn5 (RPN5 Budding Yeast)

Expression

Yeast

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pDR274-gsc2-sgRNA

Plasmid

#184818

Purpose

Used to synthesize gRNA targeting exon 2 of the gsc2 gene

Depositor

Marnie Halpern

Article

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

gsc2-sgRNA

Use

CRISPR; Template for sgrna synthesis

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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TOPO-nmbb

Plasmid

#184822

Purpose

Used to synthesize RNA probe for in situ hybridization for nmbb

Depositor

Marnie Halpern

Article

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

nmbb (nmbb Zebrafish)

Use

Template for synthesis of nmbb probe

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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TOPO-nts

Plasmid

#184823

Purpose

Used to synthesize RNA probe for in situ hybridization for nts

Depositor

Marnie Halpern

Article

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

nts (nts Zebrafish)

Use

Template for synthesis of nts probe

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pDestTol2-QUAS:NLS-GFP-he1.1:CFP

Plasmid

#184815

Purpose

Used to generate the Tg(QUAS:NLS-GFP; he1.1:CFP)c682 transgenic line

Depositor

Marnie Halpern

Article

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

QUAS:NLS-GFP-pA; he1.1:CFP

Use

Zebrafish tol2 transgenesis

Tags

NLS-GFP

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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SHLD3 N-terminal sgRNA

Plasmid

#207094

Purpose

pX330 based plasmid for expression of Cas9 and the TTACTGCAGAATGACTACAG sgRNA to target the SHLD3 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

TTACTGCAGAATGACTACAG

Expression

Mammalian

Promoter

CMV and U6

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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NBS1 C-terminal sgRNA

Plasmid

#207092

Purpose

pX330 based plasmid for expression of Cas9 and the TAAAAAGGAGAAGATAACTG sgRNA to target the NBS1 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

TAAAAAGGAGAAGATAACTG

Expression

Mammalian

Promoter

CMV and U6

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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RNF169 C-terminal sgRNA

Plasmid

#207099

Purpose

pX330 based plasmid for expression of Cas9 and the ACACTTCATTAGGTGCTACT sgRNA to target the RNF169 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

ACACTTCATTAGGTGCTACT

Expression

Mammalian

Promoter

CMV and U6

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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SHLD1 N-terminal sgRNA

Plasmid

#207101

Purpose

pX330 based plasmid for expression of Cas9 and the ATGGCAGGACTATGGCAGCC sgRNA to target the SHLD1 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

ATGGCAGGACTATGGCAGCC

Expression

Mammalian

Promoter

CMV and U6

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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ATM C-terminal sgRNA

Plasmid

#207097

Purpose

pX330 based plasmid for expression of Cas9 and the TTTCTAAAGGCTGAATGAAA sgRNA to target the ATM locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

TTTCTAAAGGCTGAATGAAA

Expression

Mammalian

Promoter

CMV and U6

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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SHLD3 KO sgRNA #1

Plasmid

#207105

Purpose

pX330 based plasmid for expression of Cas9 and the CGCTATCAAGATTTATACCT sgRNA to target the SHLD3 locus..

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

CGCTATCAAGATTTATACCT

Expression

Mammalian

Promoter

CMV and U6

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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SHLD2 N-terminal sgRNA

Plasmid

#207098

Purpose

pX330 based plasmid for expression of Cas9 and the TTTTTATCAGAAATCATGAG sgRNA to target the SHLD2 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

TTTTTATCAGAAATCATGAG

Expression

Mammalian

Promoter

CMV and U6

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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HaloTag KO sgRNA

Plasmid

#207102

Purpose

pX330 based plasmid for expression of Cas9 and the GTCGATGTTGGTCCGCGCGA sgRNA to target the HaloTag coding sequence.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

GTCGATGTTGGTCCGCGCGA

Expression

Mammalian

Promoter

CMV and U6

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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MDC1 N-terminal sgRNA

Plasmid

#207090

Purpose

pX330 based plasmid for expression of Cas9 and the GTATCCTTCCCAGATCATGG sgRNA to target the MDC1 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

GTATCCTTCCCAGATCATGG

Expression

Mammalian

Promoter

CMV and U6

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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SHLD3 KO sgRNA #2

Plasmid

#207106

Purpose

pX330 based plasmid for expression of Cas9 and the CTGAAGGAACAGACTAATTC sgRNA to target the SHLD3 locus..

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

CTGAAGGAACAGACTAATTC

Expression

Mammalian

Promoter

CMV and U6

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pX459-canis_PTGS2-4

Plasmid

#184730

Purpose

COX2-KO in MDCK

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

A gRNA targeting the dog COX2 gene.

Use

CRISPR

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pX459-homo_PLA2G4A-1

Plasmid

#184735

Purpose

cPLA2-KO in HeLa

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

A gRNA targeting the human cPLA2 gene.

Use

CRISPR

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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