We narrowed to 11,368 results for: Lif

1. pcDNA3-ER-AKAR4

   Plasmid

   #64733

   Purpose

   Endoplasmic reticulum (ER)-targeted FRET-based PKA activity reporter; targeted to cytosolic face of ER membrane.

   Depositor

   Jin Zhang

   Article

   Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

   Insert

   AKAR4
   

   Use

   Tags

   Cerulean, N-terminal 27 amino acids from CYP450, …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp

   Plasmid

   #125228

   Purpose

   Expresses SPARK2 NanoLuc-βarrestin2-TEVp in mammalian cells

   Depositor

   Alice Ting

   Article

   Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

   Insert

   NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp
   

   Use

   AAV

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEMHE-mEGFP-mTrim21

   Plasmid

   #105519

   Purpose

   T7 promotor drives in vitro transcription of mEGFP-tagged wildtype mouse TRIM21 mRNA

   Depositor

   Melina Schuh

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   TRIM21 (Trim21 Mouse)
   

   Use

   In vitro transcription

   Tags

   mEGFP

   Expression

   Mutation

   Promoter

   T7

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR CD19-PI3K CAR

   Plasmid

   #113018

   Purpose

   for lentivirus production of CD19-PI3K CAR

   Depositor

   Ron Vale

   Article

   Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

   Insert

   CD19 CAR-P intracellular PI3k recruiting domain from CD19 (CD19 Human, Synthetic)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBXNPHM3

   Plasmid

   #110099

   Purpose

   E.coli expression vector for FX cloning system, N-terminal pelB signal sequence followed by 10xHisTag, maltose binding protein and 3C cleavage site

   Depositor

   Markus Seeger

   Article

   Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

   Type

   Empty backbone

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-EF1α-FRT-FLEX-GtACR1-EYFP

   Plasmid

   #114370

   Purpose

   expresses Flpo recombinase-dependent GtACR1

   Depositor

   Mingshan Xue

   Article

   Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

   Insert

   GtACR1-EYFP (NEWENTRY )
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-CAG-DIO-APEX2NES

   Plasmid

   #79907

   Purpose

   Adeno-associated virus plasmid, Cre-dependent FLEX switch, APEX2NES

   Depositor

   Joshua Sanes

   Article

   Joesch et al Elife. 2016 Jul 7;5. pii: e15015. doi: 1

   Insert

   APEX2NES
   

   Use

   AAV and Cre/Lox

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. CSII-IDR2-humanTRIM5-FOS

   Plasmid

   #79066

   Purpose

   Express human TRIM5alpha in mammalan cells

   Depositor

   Wesley Sundquist

   Article

   Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

   Insert

   TRIM5alpha(human)-FOS (TRIM5 Human)
   

   Use

   Tags

   OneSTrEP-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB-EF1-IRES-NEO deleted ITR

   Plasmid

   #67906

   Purpose

   PiggyBac transposon encoding Neomycin resistance with deleted inverted terminal repeat

   Depositor

   Alex Kentsis

   Article

   Henssen et al Elife. 2015 Sep 25;4. pii: e10565. doi:

   Insert

   NeoR (neoR Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-EF1α-FRT-FLEX-GtACR2-EYFP

    Plasmid

    #114369

    Purpose

    expresses Flpo recombinase-dependent GtACR2

    Depositor

    Mingshan Xue

    Article

    Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

    Insert

    GtACR2-EYFP (NEWENTRY )
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. SAC1delta452-587-FKBP-mCherry

    Plasmid

    #108131

    Purpose

    Recruitable inactive SAC1 (linker deletion)

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    SACM1L(1-451):FKBP1A(3-108):mCherry (SACM1L Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SACM1L(1-451)

    Promoter

    CMV

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. PresentER-MSIIFFLPL (GFP)

    Plasmid

    #102946

    Purpose

    PresentER minigene encoding H2-Kb ligand MSIIFFLPL (GFP)

    Depositor

    David Scheinberg

    Article

    Gejman et al Elife. 2018 Nov 30;7. pii: 41090. doi: 1

    Insert

    MSIIFFLPL (Serpinf1 Synthetic, Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. PDZD7-TwinStrepII

    Plasmid

    #83198

    Purpose

    Expression of tagged protein in mammalian cells

    Depositor

    Peter Barr-Gillespie

    Article

    Morgan et al Elife. 2016 Aug 15;5:e18312. doi: 10.755

    Insert

    PDZD7 (Pdzd7 Mouse)
    

    Use

    Tags

    Twin Strep Tag II

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-ER-CaNAR2

    Plasmid

    #64732

    Purpose

    Endoplasmic reticulum (ER)-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of ER membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CYP450-CaNAR2
    

    Use

    Tags

    Cerulean3, N-terminal 27 amino acids from CYP450,…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTY07

    Plasmid

    #83285

    Purpose

    human CENP-A N-terminally labeled with LSSmOrange

    Depositor

    Daniel Needleman

    Article

    Yoo et al Elife. 2018 Jul 25;7. pii: 36392. doi: 1

    Insert

    centromere protein A (CENPA Human)
    

    Use

    Retroviral

    Tags

    LSSmOrange

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-PM-CaNAR2

    Plasmid

    #64730

    Purpose

    Plasma membrane-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    Lyn-CaNAR2
    

    Use

    Tags

    Cerulean3, N-terminal targeting sequence from Lyn…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. lentiGuide-RRBP1-1

    Plasmid

    #92156

    Purpose

    CRISPR guide RNA targeting human RRBP1

    Depositor

    Alice Ting

    Article

    Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

    Insert

    RRBP1 sgRNA-1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. VinTS-tCRMod-GGSGGS7

    Plasmid

    #111764

    Purpose

    Expresses vinculin tension sensor comprised of Clover-(GGSGGS)7-mRuby2 module

    Depositor

    Brenton Hoffman

    Article

    LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

    Insert

    Clover-mRuby2-based vinculin tension sensor with (GGSGGS)7 extensible domain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Residues 1-228 of Clover, residues 1-3 deleted fr…

    Promoter

    CMV

    Available since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-EF1a-Flp-DOG-NW

    Plasmid

    #75469

    Purpose

    Flp recombinase dependent on GFP (Flp-DOG) for rAAV production and expression in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

    Has Service

    AAV1

    Insert

    Flp-DOG
    

    Use

    AAV and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-EF1α-DIO-ReaChR-P2A-dTomato

    Plasmid

    #114368

    Purpose

    expresses a Cre-dependent red light-shifted channelrhodopsin variant

    Depositor

    Mingshan Xue

    Article

    Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

    Insert

    ReaChR-P2A-dTomato
    

    Use

    AAV

    Tags

    dTomato

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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