We narrowed to 162,256 results for: addgene

1. pFUGW-scrambled

   Plasmid

   #183455

   Purpose

   This control vector contains a scrambled version of the targeting sequence used in the pFUGW-shRIIα construct

   Depositor

   Matthew Gold

   Article

   Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

   Insert

   scrambled sgRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   shRNA: H1 / gene: ubiquitin

   Available since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330_gRNA_IG-DMR-1

   Plasmid

   #159931

   Purpose

   Specific gRNA against mouse IG-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of IG-DMR in mouse ESC.

   Depositor

   Anton Wutz

   Article

   Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

   Insert

   gRNA mouse IG-DMR
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, CBh

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330_gRNA_IG-DMR-2

   Plasmid

   #159932

   Purpose

   Specific gRNA against mouse IG-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of IG-DMR in mouse ESC.

   Depositor

   Anton Wutz

   Article

   Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

   Insert

   gRNA mouse IG-DMR
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, CBh

   Available since

   Nov. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLIK_hygro_p53(R280K)_V5

   Plasmid

   #136540

   Purpose

   Lentiviral expression vector for an inducible p53(R280K)-V5

   Depositor

   Kevin Janes

   Article

   Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

   Insert

   p53(R280K) (TP53 Human)
   

   Use

   Lentiviral; Destinatioin vector for gateway cloni…

   Tags

   V5

   Expression

   Mammalian

   Mutation

   p53(R280K)V5 was cloned from pLenti6-p53-R280K-V5…

   Promoter

   Available since

   June 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEN_TTmiRc2_p53(R280K)_V5

   Plasmid

   #136525

   Purpose

   Used as a donor vector to clone into pSLIK

   Depositor

   Kevin Janes

   Article

   Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

   Insert

   p53(R280K) (TP53 Human)
   

   Use

   Entry vector for gateway cloning

   Tags

   V5

   Expression

   Mutation

   p53(R280K)V5 was cloned from pLenti6-p53-R280K-V5…

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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6. 7TFC

   Plasmid

   #24307

   Depositor

   Roel Nusse

   Article

   Fuerer et al PLoS One. 2010 . 5(2):e9370.

   Insert

   7xTcf-FFluc // SV40-mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA

   Plasmid

   #154868

   Purpose

   Human synapsin-1 promoter; Flp-dependent hM3D(Gq) DREADD-mCherry

   Depositor

   Ulrik Gether

   Has Service

   AAV Retrograde and AAV8

   Insert

   hM3D(Gq)-mCherry
   

   Use

   AAV; Flp-frt

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Human Synapsin-1

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTF275 (pFA6a-TEV-6xGly-2xStrep-His3MX)

   Plasmid

   #44070

   Depositor

   Tim Formosa

   Type

   Empty backbone

   Use

   Pcr-based yeast c-terminal tagging

   Tags

   2xStrep tag, 6xGly linker, ADH1 transcription ter…

   Expression

   Mutation

   Promoter

   Available since

   April 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTF263 (pFA6a-TEV-6xGly-12xHis-His3MX)

   Plasmid

   #44069

   Depositor

   Tim Formosa

   Type

   Empty backbone

   Use

   Pcr-based yeast c-terminal tagging

   Tags

   12xHis tag, 6xGly linker, ADH1 transcription term…

   Expression

   Mutation

   Promoter

   Available since

   April 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV V5-lamin B1

    Plasmid

    #175108

    Purpose

    Lentiviral expression of V5-tagged mouse Lmnb1

    Depositor

    Larry Gerace

    Insert

    Lmnb1 (Lmnb1 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. PERK 6: PERK.9E10.pBABE

    Plasmid

    #21819

    Depositor

    David Ron

    Insert

    PERK (Eif2ak3 Mouse)
    

    Use

    Retroviral

    Tags

    Myc (9E10)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-Ef1a-fDIO-JRGECO1a

    Plasmid

    #128317

    Purpose

    Expresses JRGECO1a under control of eukaryotic Ef1a promoter after Flp-dependent recombination.

    Depositor

    Patricia Jensen

    Has Service

    AAV1

    Insert

    NES-JRGECO1a
    

    Use

    AAV

    Tags

    His Tag

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. MSCV-Jarid1c-IRES-GFP

    Plasmid

    #166009

    Purpose

    Retroviral biscistronic expression of Jarid1c and eGFP

    Depositor

    Manu Rangachari

    Insert

    Jarid1c (Kdm5c Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. UHRF1

    Plasmid

    #28163

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    UHRF1 (UHRF1 Human)
    

    Use

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    tandem tudor domain

    Promoter

    Available since

    Feb. 28, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

15. pUltra-Plus-H2B-mTagBFP2

    Plasmid

    #161790

    Purpose

    3rd generation lentiviral bicistronic vector for mammalian expression of H2B-mTagBFP2 and one gene of interest

    Depositor

    Jorge Luis Arias-Arias

    Insert

    H2B-mTagBFP2
    

    Use

    Lentiviral; Bicistronic

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    I174A in the mTagBFP2 protein according to PLoS O…

    Promoter

    UbC and UbC promoter

    Available since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. meGFP-hTRAK1

    Plasmid

    #188664

    Purpose

    human TRAK1 with N-terminal meGFP tag

    Depositor

    Thomas Schwarz

    Insert

    OIP106 (TRAK1 Human)
    

    Use

    Tags

    meGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-BE4max-P2A-EGFP (pRZ33)

    Plasmid

    #140083

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with P2A-EGFP

    Depositor

    Keith Joung

    Insert

    Human codon optimized pCAG BE4max with P2A-EGFP
    

    Use

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    D10A (SpCas9)

    Promoter

    CAG

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CMV-FLEX-SaCas9-U6-sgOprk1

    Plasmid

    #159903

    Purpose

    Mutagenesis of Oprk1

    Depositor

    Larry Zweifel

    Insert

    Oprk1 (Oprk1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAVS1_Puro_hPGK1_hGEM(1-110)_mNG_hSLBP(18-108)

    Plasmid

    #176932

    Purpose

    Donor for targeted integration of S-phase specific probe (FUCCI-S) to the AAVS1 safe harbor locus

    Depositor

    Yannick Doyon

    Insert

    hGEM(1-110)_mNG_hSLBP(18-108)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK1

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-HA_human_BAP1

    Plasmid

    #158109

    Purpose

    Expresses tagged BAP1, neomycin selection

    Depositor

    Emanuela Felley-Bosco

    Insert

    BAP1 (BAP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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