We narrowed to 165,694 results for: addgene

1. pFUGW-scrambled

   Plasmid

   #183455

   Purpose

   This control vector contains a scrambled version of the targeting sequence used in the pFUGW-shRIIα construct

   Depositor

   Matthew Gold

   Article

   Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

   Insert

   scrambled sgRNA
   

   Use

   Lentiviral

   Promoter

   shRNA: H1 / gene: ubiquitin

   Available Since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330_gRNA_IG-DMR-1

   Plasmid

   #159931

   Purpose

   Specific gRNA against mouse IG-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of IG-DMR in mouse ESC.

   Depositor

   Anton Wutz

   Article

   Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

   Insert

   gRNA mouse IG-DMR
   

   Use

   CRISPR and Mouse Targeting

   Expression

   Mammalian

   Promoter

   U6, CBh

   Available Since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330_gRNA_IG-DMR-2

   Plasmid

   #159932

   Purpose

   Specific gRNA against mouse IG-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of IG-DMR in mouse ESC.

   Depositor

   Anton Wutz

   Article

   Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

   Insert

   gRNA mouse IG-DMR
   

   Use

   CRISPR and Mouse Targeting

   Expression

   Mammalian

   Promoter

   U6, CBh

   Available Since

   Nov. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLIK_hygro_p53(R280K)_V5

   Plasmid

   #136540

   Purpose

   Lentiviral expression vector for an inducible p53(R280K)-V5

   Depositor

   Kevin Janes

   Article

   Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

   Insert

   p53(R280K) (TP53 Human)
   

   Use

   Lentiviral; Destinatioin vector for gateway cloni…

   Tags

   V5

   Expression

   Mammalian

   Mutation

   p53(R280K)V5 was cloned from pLenti6-p53-R280K-V5…

   Available Since

   June 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEN_TTmiRc2_p53(R280K)_V5

   Plasmid

   #136525

   Purpose

   Used as a donor vector to clone into pSLIK

   Depositor

   Kevin Janes

   Article

   Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

   Insert

   p53(R280K) (TP53 Human)
   

   Use

   Entry vector for gateway cloning

   Tags

   V5

   Mutation

   p53(R280K)V5 was cloned from pLenti6-p53-R280K-V5…

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAS_MmaPylT_EF1_NES-MmaPylRS WT

   Plasmid

   #174901

   Purpose

   expression of Mma PylRS with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integration

   Depositor

   Simon Elsaesser

   Insert

   Mma PylRS (pylS Methanosarcina mazei)
   

   Tags

   FLAG and NES

   Expression

   Mammalian

   Promoter

   EF1

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA

   Plasmid

   #154868

   Purpose

   Human synapsin-1 promoter; Flp-dependent hM3D(Gq) DREADD-mCherry

   Depositor

   Ulrik Gether

   Has Service

   AAV Retrograde and AAV8

   Insert

   hM3D(Gq)-mCherry
   

   Use

   AAV; Flp-frt

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   Human Synapsin-1

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUltra-Plus-H2B-mTagBFP2

   Plasmid

   #161790

   Purpose

   3rd generation lentiviral bicistronic vector for mammalian expression of H2B-mTagBFP2 and one gene of interest

   Depositor

   Jorge Luis Arias-Arias

   Insert

   H2B-mTagBFP2
   

   Use

   Lentiviral; Bicistronic

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   I174A in the mTagBFP2 protein according to PLoS O…

   Promoter

   UbC and UbC promoter

   Available Since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pZC06 (pFA6a-PSc-6xGly-PrtA-KanMX)

   Plasmid

   #44090

   Depositor

   Tim Formosa

   Type

   Empty backbone

   Use

   Pcr-based yeast c-terminal tagging

   Tags

   6xGly linker, ADH1 transcription terminator, KanM…

   Available Since

   April 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV CMV FST-FLAG

    Plasmid

    #175167

    Purpose

    Lentiviral expression of human FST-FLAG

    Depositor

    Larry Gerace

    Insert

    FST (FST Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Jan. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. 7TFC

    Plasmid

    #24307

    Depositor

    Roel Nusse

    Article

    Fuerer et al PLoS One. 2010 . 5(2):e9370.

    Insert

    7xTcf-FFluc // SV40-mCherry
    

    Use

    Lentiviral

    Available Since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pWT015a

    Plasmid

    #96878

    Purpose

    GFP reporter

    Depositor

    David Liu

    Article

    Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

    Insert

    GFP
    

    Expression

    Mammalian

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-Ef1a-fDIO-JRGECO1a

    Plasmid

    #128317

    Purpose

    Expresses JRGECO1a under control of eukaryotic Ef1a promoter after Flp-dependent recombination.

    Depositor

    Patricia Jensen

    Has Service

    AAV1

    Insert

    NES-JRGECO1a
    

    Use

    AAV

    Tags

    His Tag

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. PERK 6: PERK.9E10.pBABE

    Plasmid

    #21819

    Depositor

    David Ron

    Insert

    PERK (Eif2ak3 Mouse)
    

    Use

    Retroviral

    Tags

    Myc (9E10)

    Expression

    Mammalian

    Available Since

    March 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV V5-lamin B1

    Plasmid

    #175108

    Purpose

    Lentiviral expression of V5-tagged mouse Lmnb1

    Depositor

    Larry Gerace

    Insert

    Lmnb1 (Lmnb1 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. MSCV-Jarid1c-IRES-GFP

    Plasmid

    #166009

    Purpose

    Retroviral biscistronic expression of Jarid1c and eGFP

    Depositor

    Manu Rangachari

    Insert

    Jarid1c (Kdm5c Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. meGFP-hTRAK1

    Plasmid

    #188664

    Purpose

    human TRAK1 with N-terminal meGFP tag

    Depositor

    Thomas Schwarz

    Insert

    OIP106 (TRAK1 Human)
    

    Tags

    meGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTF275 (pFA6a-TEV-6xGly-2xStrep-His3MX)

    Plasmid

    #44070

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    2xStrep tag, 6xGly linker, ADH1 transcription ter…

    Available Since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTF263 (pFA6a-TEV-6xGly-12xHis-His3MX)

    Plasmid

    #44069

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    12xHis tag, 6xGly linker, ADH1 transcription term…

    Available Since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. UHRF1

    Plasmid

    #28163

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    UHRF1 (UHRF1 Human)
    

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    tandem tudor domain

    Available Since

    Feb. 28, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits