Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 5,317 results for: golden gate

Clear keywords
Showing: 1161 - 1180 of 5317 results

  4. pNM3749

    Plasmid
    #154108
    Purpose
    loxP FRT FRT3 RMCE landing site in a Golden Gate SapI GCG ACG assembly vector
    Depositor
    Michael Nonet
    Publication
    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.
    Inserts
    LoxP-SEC-LoxP
    FRT::rpl-28p::GFP::his-58::FRT3
    Use
    Cre/Lox; Rmce
    Expression
    Worm
    Promoter
    not applicable
    Available since
    July 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    154108-plasmid-map-sequence-id-306067

    Enlarge

  10. pGGD_MiniTurboID-YFP

    Plasmid
    #222434
    Purpose
    Golden Gate / Green Gate module for adding MiniTurboID and YFP as C-terminus of target protein.
    Depositor
    Dominique Bergmann
    Publication
    Wallner et al Dev Cell. 2024 May 6;59(9):1096-1109.e5.
    Insert
    MiniTurboID (BirA mutant)
    Use
    Golden gate / green gate compatible cloning vector
    Tags
    Linker and YFP
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    222434-plasmid-map-sequence-id-440831

    Enlarge

  11. pGGB_YFP-MiniTurboID

    Plasmid
    #222433
    Purpose
    Golden Gate / Green Gate module for adding YFP and MiniTurboID as N-terminus of target protein.
    Depositor
    Dominique Bergmann
    Publication
    Wallner et al Dev Cell. 2024 May 6;59(9):1096-1109.e5.
    Insert
    MiniTurboID (BirA mutant)
    Use
    Golden gate / green gate compatible cloning vector
    Tags
    Linker and YFP
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    222433-plasmid-map-sequence-id-440826

    Enlarge

  12. CyanoGate Kit

    Plasmid kit
    #1000000146
    Purpose
    Vectors for generating multi-part assemblies in either integrative (suicide) or self-replicating plasmid vectors for working in cyanobacteria.
    Depositor
    Alistair McCormick
    Application
    Cloning and Synthetic Biology
    Vector type
    Bacterial Expression
    Cloning type
    Golden Gate (MoClo)
    Available since
    Jan. 14, 2020
    Availability
    Academic Institutions and Nonprofits only

  13. IBMc180

    Plasmid
    #161942
    Purpose
    Carries Golden Gate substitution insert for acVHH-assisted bisection mapping
    Depositor
    Baojun Wang
    Publication
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB1A3-BbsI-10aa-acVHH-T-cat-PBAD(SapI)-RiboJ-BCD22-acVHH-10aa-SapI
    Expression
    Bacterial
    Promoter
    PBAD
    Available since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    161942-plasmid-map-sequence-id-329042

    Enlarge

  14. IBMc050

    Plasmid
    #161938
    Purpose
    Carries Golden Gate substitution insert for IBM with M86 intein. Plux2 as C-lobe promoter
    Depositor
    Baojun Wang
    Publication
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB1A3-BbsI-M86N-cat-Plux2-B32-M86C-SapI
    Expression
    Bacterial
    Promoter
    Plux2
    Available since
    March 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    161938-plasmid-map-sequence-id-327548

    Enlarge

Showing: 1161 - 1180 of 5317 results