We narrowed to 1,687 results for: plasmid for e coli
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pJL NarX Myc
Plasmid#225651PurposeExpresses WT NarX capable of sensing nitrate. For use with NarL-YdfI and reporter plasmids from this study.DepositorInsertNarX (narX E. coli)
UseCell free expressionTagsMycExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCL.103
Plasmid#184988PurposeExpress Cas9-P2A-Eco1RTDepositorInsertCas9-P2A-Eco1RT
UseTagsSV40NLSExpressionYeastMutationhuman codon optimized RTPromoterGal1-10Available sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.027
Plasmid#184964PurposeExpress -Eco1 RT and ncRNA in yeastDepositorInsertEco1: RT and ncRNA(wt), a1/a2 length: 12
UseTagsExpressionYeastMutationhuman codon optimized RTPromoterGal7Available sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.102
Plasmid#184987PurposeExpress -Eco1 RT-P2A-Cas9DepositorInsertEco1RT-P2A-Cas9
UseTagsSV40NLSExpressionYeastMutationhuman codon optimized RTPromoterGal1-10Available sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.006
Plasmid#184970PurposeExpress -Eco1 RT in yeastDepositorInsertIntegrating inducible cassette: Eco1 RT
UseTagsSV40NLSExpressionYeastMutationhuman codon optimized RTPromoterGal1-10Available sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEC3109 (pSpy1C-Plac(1)_ffluc)
Plasmid#218514Purposereplicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressorDepositorInsertffluc
UseLuciferaseTagsExpressionBacterialMutationdeletion of mrfp cassette, mutagenesis of the RBS…PromoterPlac(1)Available sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEC3111 (pSpy1C-Plac(3)_ffluc)
Plasmid#218778Purposereplicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressorDepositorInsertffluc
UseLuciferaseTagsExpressionBacterialMutationdeletion of mrfp cassette, mutagenesis of the RBS…PromoterPlac(3)Available sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIA1385
Plasmid#166862PurposeCodon optimized SARS-CoV-2 Nsp12 gene was cloned into a modified pRSFDuet-1 vector (Addgene #159107) for expression in E. coli.DepositorInsertSARS-COV-2 Nsp12 (ORF1ab Synthetic)
UseTagsHis-SUMOExpressionBacterialMutationPromoterT7Available sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGG-TurboID-GFP-PTS1
Plasmid#209406PurposeTransiently expressing and visualizing the subcellular localization of TurboID fused with a peroxisomal targeting signal type I (PTS1) in plantaDepositorInsert3XHA-TurboID-PTS1
UseTagsGFPExpressionPlantMutationTurboID gene sequence C249APromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable sinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSEVA3b67Rb
Plasmid#58311PurposeShuttle vector: contains constitutive mRFP1, compatible as reporter for E. coli/B. subtilis. See pSEVA3b61 for more info.DepositorInsertmRFP1
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
WT FL USP7
Plasmid#227214PurposeExpresses human USP7 with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
UseTags6HisExpressionBacterialMutationPromoterT7-promoterAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMK188
Plasmid#226659PurposepCB2.4 - pBR322 shuttle vector that replicates in E. coli and Synechococcus sp. PCC 7002, contains HIP1 siteDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJan. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-3-6AOptParkin
Plasmid#165107Purposebacterial expression of a GST fusion of e.coli optimized Parkin with 6 alanine linker between the GST and the inserted Parkin geneDepositorInsertParkin isoform 1 (PRKN Human)
UseTagsGST-6-alanineExpressionBacterialMutationcodon optimized for E. coli expressionPromoterAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUCXKT
Plasmid#60682Purposepositive selection cloning and expression vectorDepositorTypeEmpty backboneUsePositive selection e. coli expression vectorTagsExpressionBacterialMutationPromotertacAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
His 3C MKK4 20-399
Plasmid#87880PurposeE. coli expression of MKK4 aa 20-399DepositorInsertMKK4 (MAP2K4 Human)
UseTags3C protease site and 6xHisExpressionBacterialMutationaa 20-399PromoterT7Available sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJB06
Plasmid#190480PurposeBase Cas9 expression plasmid for 2-plasmid C. difficile mutagenesis systemDepositorInsertsCas9
XylR
UseE. coli / b. subtilis - c. difficile shuttle vect…TagsExpressionMutationPromoterxylR-driven promoterAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJB07
Plasmid#190481PurposeTargeting plasmid for 2-plasmid C. difficile mutagenesis systemDepositorInsertsUpstream & Downstream pyrE deletion region
gRNA
UseE. coli / b. subtilis - c. difficile shuttle vect…TagsExpressionMutationPromoterAvailable sinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRL1124
Plasmid#70690Purposeprovides avaiM, eco47iiM, and ecot22iM that encode specific methylases to enable transfer of plasmids from E. coli to Anabaena sp. strain PCC 7120 and other strains. lacks a mobilization function.DepositorInsertsavaiM
eco47iiM
ecot22iM
UseBacterial shuttle vectorTagsExpressionMutationPromoterAvailable sinceNov. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMM643
Plasmid#112533PurposepZE2-PLhrtO-lux-hrtR-RBS3-chuA - HrtR RBS variant of plasmid pMM627 (Strength 33545.5 AU), ColE1 origin, KanRDepositorInsertsHrtR
ChuA
luxCDABE
UseTagsExpressionBacterialMutationA17PPromoterJ23107, PL(HrtO), and ProDAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only
