We narrowed to 20,724 results for: ACE;

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pCm N-term MBP NikJC199U

Plasmid

#174364

Purpose

Expression plasmid with a TEV removable N-term MBP tag, a TEV removable C-term 8xHis tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.

Depositor

Brandon Greene

Article

Caceres et al Methods Enzymol. 2022;662:119-141. doi:

Insert

nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]

Tags

8xHis, MBP, and TEV

Expression

Bacterial

Promoter

T7

Available Since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD-deLACCO

Plasmid

#167945

Purpose

Fluorescent biosensor control for extracellular L-lactate

Depositor

Robert Campbell

Article

Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

Insert

deLACCO

Expression

Bacterial

Promoter

araBAD

Available Since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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pMP89b CvTA V124N

Plasmid

#141207

Purpose

Chromobacterium violaceum transaminase with enhances affinity for PLP. TEV cleaving site after the N-terminal Histag.

Depositor

Francesca Paradisi

Article

Roura Padrosa et al Front Bioeng Biotechnol. 2019 Oct 18;7:2

Insert

CvTA

Expression

Bacterial

Mutation

V124N

Available Since

June 15, 2020

Availability

Academic Institutions and Nonprofits only

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pcF-2

Plasmid

#131025

Purpose

Single spacer array with spacer #2 for FnCas12a.

Depositor

Chase Beisel

Article

Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

Insert

single spacer array for FnCas12a

Use

CRISPR

Expression

Bacterial

Promoter

PJ23119

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pcF-3

Plasmid

#131024

Purpose

Single spacer array with spacer #3 for FnCas12a.

Depositor

Chase Beisel

Article

Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

Insert

single spacer array for FnCas12a

Use

CRISPR

Expression

Bacterial

Promoter

PJ23119

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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shCDK4(1)

Plasmid

#73550

Purpose

shRNA(1) against CDK4

Depositor

Gerardo Ferbeyre

Article

Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

Insert

shRNA against CDK4

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only
shCDK4(2)

Plasmid

#73551

Purpose

shRNA(2) against CDK4

Depositor

Gerardo Ferbeyre

Article

Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

Insert

shRNA against CDK4

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only
pJexpress414_OmpA171_WTnoGirdles

Plasmid

#62653

Purpose

Wild-type OmpA with aromatic girdle residues replaced by leucine

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA_noGirdles

Use

Synthetic Biology

Tags

6His and FlAsH

Expression

Bacterial

Mutation

Aromatic girdle residues mutated to Leu (W7, W15,…

Promoter

T7

Available Since

May 27, 2015

Availability

Academic Institutions and Nonprofits only
pAKW.114A-PKA

Plasmid

#154051

Purpose

Expresses PKA in E. coli

Depositor

Christopher Voigt

Article

Wallace et al Advanced Functional Materials 2020

Insert

Protein kinase cAMP-activated catalytic subunit alpha (PKA) (PRKACA Human)

Expression

Bacterial

Promoter

pCin

Availability

Academic Institutions and Nonprofits only

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pNICKclos1.0

Plasmid

#73639

Purpose

Genome editing for gene pyrE (CAC-002) in Clostridium acetobutylicum ATCC 824

Depositor

Sheng Yang

Article

Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

Inserts

Cas9 nickase
sGRNA to pyrE

Use

E.coli-clostridium shuttle vector

Mutation

D10A

Promoter

j23119 and ptb

Available Since

June 17, 2016

Availability

Academic Institutions and Nonprofits only

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Perturb-FISH

Plasmid

#220626

Purpose

expression of gRNAs under U6T7 promoter

Depositor

Samouil Farhi

Article

Binan et al Cell. 2025 Mar 10:S0092-8674(25)00197-7.

Insert

sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

BsmBI

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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HDAC5 Flag

Plasmid

#13822

Purpose

Mammalian expression of human histone deacetylase 5 with flag tag

Depositor

Eric Verdin

Article

Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

Insert

HDAC5 (HDAC5 Human)

Tags

flag

Expression

Mammalian

Available Since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

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alpha7nACHR_Nacho_GCAMP7s

Plasmid

#182940

Purpose

alpha7 nACHR expression in mammalian cell lines with calcium reporter

Depositor

Robert Brenner

Article

Belanger-Coast et al Eur J Pharmacol. 2022 Jun 15;925:175000.

Insert

NACH7 (CHRNA7 Human)

Expression

Mammalian

Promoter

EF1alpha

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pZac2.1-GfaABC1D-TurboID(full)-HA-GPI

Plasmid

#166055

Purpose

Expresses the full length TurboID on the surface of astrocytes

Depositor

Scott Soderling

Article

Takano et al Nature. 2020 Dec;588(7837):296-302. doi:

Insert

GPI anchored TurboID

Use

AAV

Available Since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pAEMXT-eLACCO1.1

Plasmid

#167946

Purpose

Fluorescent biosensor for extracellular L-lactate

Depositor

Robert Campbell

Article

Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

Insert

CD59-eLACCO1.1-GPI

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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SQT1665

Plasmid

#78744

Purpose

Human expression plasmid for LbCpf1-NLS-3xHA

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

Insert

mammalian codon-optimized Lachnospiraceae bacterium ND2006 Cpf1-NLS-3xHA

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Promoter

CAG

Available Since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

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Plasmid EUPp_09

Plasmid

#157745

Purpose

PgyrA_ada _adh2 _pMB1_ampR (for expressing two enzymes (Ada and Adh2) to allow E. coli to consume ethanol)

Depositor

Kang Zhou

Article

Liang et al Metab Eng. 2020 Nov 25. pii: S1096-7176(

Inserts

acetaldehyde dehydrogenase
alcohol dehydrogenase (ADH2 Budding Yeast)

Expression

Bacterial

Promoter

PgyrA

Available Since

June 4, 2021

Availability

Academic Institutions and Nonprofits only

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IMS-HyPer2

Plasmid

#60248

Purpose

Genetically-encoded fluorescent hydrogen peroxide indicator HyPer2 targeted to mitochondrial intermembrane spase.

Depositor

Vsevolod Belousov

Article

Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

Insert

IMS-HyPer2

Tags

Mitochondrial intermembrane space targeting signa…

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-eLACCO1.1

Plasmid

#168788

Purpose

Neuronal expression of fluorescent sensor for extracellular lactate

Depositor

Marie-Eve Paquet

Article

Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

Insert

eLACCO1.1

Use

AAV

Tags

ER export and GPI

Expression

Mammalian

Promoter

hSyn

Available Since

April 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-eLACCO1.1

Plasmid

#168789

Purpose

Fluorescent sensor for extracellular lactate

Depositor

Marie-Eve Paquet

Article

Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

Insert

eLACCO1.1

Use

AAV

Tags

ER export and GPI

Expression

Mammalian

Promoter

CAG

Available Since

April 28, 2021

Availability

Academic Institutions and Nonprofits only

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