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pAAV-synP-FLEX-splitTVA-EGFP

Plasmid

#52474

Purpose

Expresses splitTVA-EGFP

Depositor

Ian Wickersham

Publication

Kohara et al Nat Neurosci. 2014 Feb;17(2):269-79. doi

Insert

splitTVA-P2A-EGFP

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

Synapsin-1

Available since

April 14, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMXs-ms-Sox3

Plasmid

#50779

Purpose

retroviral expression of mouse Sox3

Depositor

Shinya Yamanaka

Publication

Nakagawa et al Nat Biotechnol. 2008 Jan;26(1):101-6. Ep

Insert

Sox3 (Sox3 Mouse)

Use

Retroviral

Expression

Mammalian

Available since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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pMXs-ms-Sox7

Plasmid

#50780

Purpose

retroviral expression of mouse Sox7

Depositor

Shinya Yamanaka

Publication

Nakagawa et al Nat Biotechnol. 2008 Jan;26(1):101-6. Ep

Insert

Sox7 (Sox7 Mouse)

Use

Retroviral

Expression

Mammalian

Available since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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SeeSaw Reporter (SSR1.0)

Plasmid

#50392

Purpose

SSR1.0 has a one I-SceI target site for generate specific double strand break after I-SceI endonuclease expression.

Depositor

Pablo Huertas Sánchez

Publication

Gomez-Cabello et al PLoS One. 2013 Oct 14;8(10):e77206. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SSR1.0

Expression

Mammalian

Promoter

CMV

Available since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only

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TN-XXL pcDNA3

Plasmid

#45797

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Publication

Mank et al Nat Methods. 2008 Sep;5(9):805-11. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TN-XXL

Tags

ECFP and YFP

Expression

Mammalian

Promoter

CMV

Available since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pBluescript-mouse eIF2C2

Plasmid

#50354

Purpose

Mouse eIF2C2 cDNA clone

Depositor

Kumiko Ui-Tei

Publication

Doi et al Curr Biol. 2003 Jan 8;13(1):41-6.

Insert

eIF2C2 (Ago2 Mouse)

Expression

Bacterial

Promoter

T7

Available since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pFA6a-G9-5FLAG-hphMX6

Plasmid

#49185

Purpose

genomic G9-5FLAG epitope tagging in S. pombe

Depositor

Eishi Noguchi

Publication

Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

Type

Empty backbone

Tags

G9-5FLAG

Expression

Yeast

Available since

Nov. 13, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAC92-pmax-dCas9VP96

Plasmid

#48224

Purpose

dCas9VP96 on pmax expression vector

Depositor

Rudolf Jaenisch

Publication

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9(D10A;H840A) fusion with VP96 activation domain

Use

CRISPR

Tags

HA Tag and VP96

Expression

Mammalian

Mutation

D10A;H840A

Promoter

CAGGS

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-Miro1 S432N

Plasmid

#47893

Purpose

Expresses myc tagged Miro1 S432N dominant negative mutant

Depositor

Pontus Aspenström

Publication

Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Miro1 S432N (RHOT1 Human)

Tags

myc

Expression

Mammalian

Mutation

S432N, dominant negative mutant

Promoter

CMV

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pGoldenGate-SE9

Plasmid

#47677

Purpose

Binary vector for Arabidopsis transformation with BsaI Golden Gate sites and Kanamycin selection.

Depositor

Jose Dinneny

Publication

Emami et al Front Plant Sci. 2013 Sep 2;4:339. doi:

Type

Empty backbone

Use

Arabidopsis transformation

Available since

Sept. 9, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 3xFlag-ERK2

Plasmid

#47573

Purpose

Bacterial expression plasmid for GST-3xFlag-tagged ERK2

Depositor

Kevin Janes

Publication

Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

Insert

ERK2 (Mapk1 Rat)

Tags

3xFlag and GST

Expression

Bacterial

Available since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pattB-DSCP-QF#7-hsp70

Plasmid

#46133

Depositor

Christopher Potter

Publication

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

QF#7

Expression

Insect

Available since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGEX PLCg1(NC)-SH2

Plasmid

#46471

Depositor

Bruce Mayer

Publication

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

PLCg1(NC) (PLCG1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 542-759

Promoter

Tac

Available since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pNC1011

Plasmid

#42898

Depositor

Beverly Errede

Publication

Houser et al Yeast. 2012 Dec;29(12):519-30. doi: 10.1

Insert

3 tandem copies of GFP*

Use

Yeast integrating

Mutation

changed Phenylalanine 64 to Leucine, Serine 65 t…

Promoter

None

Available since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Gata1

Plasmid

#41085

Depositor

Rudolf Jaenisch

Publication

Buganim et al Cell Stem Cell. 2012 Sep 7;11(3):373-86.

Insert

Gata1 (Gata1 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TetO + minimal CMV

Available since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only
pRMH120

Plasmid

#40593

Purpose

Expresses Gal4BD-CRY2PHR and Gal4AD-CIB1 for light-inducible protein expression

Depositor

Chandra Tucker

Publication

Hughes et al Methods. 2012 Aug 15.

Inserts

CRY2 (CRY2 Mustard Weed)
CIB1 (CIB1 Mustard Weed)

Expression

Yeast

Promoter

ADH1 and TEF

Available since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEGFP-C1 hSlp2-a

Plasmid

#40047

Depositor

Fernando Martin-Belmonte

Publication

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hSlp2-a (SYTL2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV promoter

Available since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pGEX-p53

Plasmid

#39479

Depositor

Ie-Ming Shih

Publication

Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

Insert

TP53 (TP53 Human)

Tags

GST

Expression

Bacterial

Mutation

P72R

Available since

Sept. 25, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pJC53.2/PRMT5

Plasmid

#35692

Depositor

Phillip Newmark

Publication

Rouhana et al Development. 2012 Mar;139(6):1083-94. Ep

Insert

Protein arginine methyltransferase 5

Promoter

Sp6 for sense, T7 for sense and antisense

Available since

June 14, 2012

Availability

Academic Institutions and Nonprofits only

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USP19 (C506A)-FLAG/pRK5

Plasmid

#36307

Depositor

Xiaolu Yang

Publication

Mei et al J Biol Chem. 2011 Oct 14;286(41):35380-7

Insert

ubiquitin specific peptidase 19 (USP19 Human)

Tags

FLAG

Expression

Mammalian

Mutation

C506A

Promoter

CMV

Available since

May 11, 2012

Availability

Academic Institutions and Nonprofits only