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pCMV-dCas12a-A3B

Plasmid

#193642

Purpose

Expression of base editor dCas12a-A3B in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Publication

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-hA3B, EGFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas12a-A3G

Plasmid

#193643

Purpose

Expression of base editor dCas12a-A3G in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Publication

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-hA3G, EGFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-ACE2 receptor 19-615

Plasmid

#167012

Purpose

Gateway-compatible Entry vector encoding ACE2 receptor (aa19-615) interacting with spike (S1) RBD domain from SARS-CoV-2

Depositor

Spyros Petrakis

Publication

Gkekas et al J Chem Inf Model. 2024 Nov 13. doi: 10.1

Insert

ACE2 (ACE2 Human)

Expression

Mammalian

Mutation

Amino acids 19-615, No stop codon

Available since

March 29, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV_Donor/Reporter-COL4A5

Plasmid

#130280

Purpose

COL4A5 mutation-specific sgRNA under the control of U6 promoter; mCherry-sgRNA target-(out of frame)EGFP expression cassette; COL4A5 donor template

Depositor

Silvestro Conticello

Publication

Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

Insert

mCherry-eGFP

Use

AAV

Promoter

CMV

Available since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX_307-SOD1E133insTT

Plasmid

#83446

Purpose

Mammalian expression of SOD1E133insTT.

Depositor

Mohan Babu

Publication

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

SOD1 (SOD1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

E133insTT (after GA)

Promoter

EF-1α

Available since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pLEX_307-SOD1E133Δ

Plasmid

#83445

Purpose

Mammalian expression of SOD1E133Δ.

Depositor

Mohan Babu

Publication

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

SOD1 (SOD1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

E133Δ

Promoter

EF-1α

Available since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pSOD1E133K-AcGFP1

Plasmid

#83442

Purpose

Expresses SOD1E133K in mammalian cells.

Depositor

Mohan Babu

Publication

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

SOD1 (SOD1 Human)

Tags

AcGFP1

Expression

Mammalian

Mutation

E133K

Promoter

CMV

Available since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pUG-UBA1

Plasmid

#104665

Purpose

Cloning Module

Depositor

Marco Trujillo

Publication

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

AtUBA1 (UBA1 Mustard Weed)

Use

Synthetic Biology

Mutation

BpiI/BsaI sites removed by point-mutation

Available since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pSB819-PKR-gib

Plasmid

#20031

Depositor

Harmit Malik

Publication

Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

Insert

Protein kinase R

Expression

Yeast

Available since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
pET29a-hNABP2

Plasmid

#128307

Purpose

Express hNABP2 (a.k.a hSSB1 or SOSSB1) in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Publication

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

Human Nucleic Acid Binding Protein 2 (hNABP2) (NABP2 Human)

Tags

His

Expression

Bacterial

Mutation

Y85C- please see depositor comments below

Promoter

T7

Available since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMXs-hs-HRAS

Plasmid

#52730

Purpose

retroviral expression of human HRAS

Depositor

Shinya Yamanaka

Publication

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

HRAS (HRAS Human)

Use

Retroviral

Expression

Mammalian

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only
pRUTH5-GST_SNRPD3-StrepII

Plasmid

#258260

Purpose

E. coli expression of human SNRPD3 C-terminal domain with an N-terminal cleavable His-GST tag and a C-terminal Strep-tag II

Depositor

David Shechter

Publication

DeAngelo et al Mol Cell. 2025 Nov 6;85(21):4016-4031.e9

Insert

SNRPD3 (SNRPD3 Human)

Tags

6xHis-GST-TEV and Strep-tag II

Expression

Bacterial

Available since

July 28, 2026

Availability

Academic Institutions and Nonprofits only

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pRUTH5-GST_SNRPD1-StrepII

Plasmid

#258259

Purpose

E. coli expression of human SNRPD1 C-terminal domain with an N-terminal cleavable His-GST tag and a C-terminal Strep-tag II

Depositor

David Shechter

Publication

DeAngelo et al Mol Cell. 2025 Nov 6;85(21):4016-4031.e9

Insert

SNRPD1 (SNRPD1 Human)

Tags

6xHis-GST-TEV and Strep-tag II

Expression

Bacterial

Available since

July 8, 2026

Availability

Academic Institutions and Nonprofits only

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pRUTH5-GST_SNRPB-StrepII

Plasmid

#258258

Purpose

E. coli expression of human SNRPB C-terminal domain with an N-terminal cleavable His-GST tag and a C-terminal Strep-tag II

Depositor

David Shechter

Publication

DeAngelo et al Mol Cell. 2025 Nov 6;85(21):4016-4031.e9

Insert

SNRPB (SNRPB Human)

Tags

6xHis-GST-TEV and Strep-tag II

Expression

Bacterial

Available since

July 8, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2 frD_ΔrE/GFP

Plasmid

#253120

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of envelope (residual AA). GFP is inserted in the locus of envelope

Depositor

Thomas Klimkait

Publication

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

Fragment D2 SARS-CoV-2 ΔE-GFP

Expression

Bacterial

Available since

June 9, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD ΔS/VSVG ΔE67 3*

Plasmid

#253173

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of spike, envelope, orf6, orf7a;stop codon at AA213 in orf3a; VSV glycoprotein replaces spike

Depositor

Thomas Klimkait

Publication

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD with VSV-G

Expression

Bacterial

Available since

June 9, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_ frD_Δ3E/VSVG Δ67a

Plasmid

#253172

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of envelope, orf6, orf7a;stop codon at AA213 in orf3a; VSV glycoprotein replaces orf3a and envelope

Depositor

Thomas Klimkait

Publication

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD with VSV-G

Expression

Bacterial

Available since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_ frD_Δ3E/N1 PR8 Δ67a

Plasmid

#253169

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of orf3a, envelope, orf6, orf7a; Influenza N1 (PR8) replaces orf3a and envelope

Depositor

Thomas Klimkait

Publication

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD with Influenza N1 PR8

Expression

Bacterial

Available since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_ frD_Δ3E/H1 PR8 Δ67a

Plasmid

#253168

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of orf3a, envelope, orf6, orf7a; Influenza H1 (PR8) replaces orf3a and envelope

Depositor

Thomas Klimkait

Publication

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD with Influenza H1 PR8

Expression

Bacterial

Available since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD2 ΔE7 3* Δ6/H1 PR8

Plasmid

#253147

Purpose

For recombinant virus rescue; encodes fragment D2 of SARS-CoV-2 (nt25422-29867), with deletion of envelope, orf6, orf7a;stop codon at AA213 in orf3a; Influenza H1 (PR8) replaces orf6

Depositor

Thomas Klimkait

Publication

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD2 with Influenza H1 PR8

Expression

Bacterial

Available since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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