Addgene

We narrowed to 207 results for: LbCas12a

Showing: 101 - 120 of 207 results

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pCFD8

Plasmid

#140619

Purpose

Expression of one of several LbCas12a crRNAs in Drosophila

Depositor

Michael Boutros

Article

Port et al Proc Natl Acad Sci U S A. 2020 Aug 25. p

Type

Empty backbone

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Drosophila U6:3

Available since

Oct. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ132D-RZ-Lb

Plasmid

#134348

Purpose

Golden Gate entry vector; empty vector to clone the 2nd LbCas12a crRNA under OsU3 promoter

Depositor

Yiping Qi

Article

Li et al Plant Biotechnol J. 2019 Nov 15. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ131C-RZ-Lb

Plasmid

#134347

Purpose

Golden Gate entry vector; empty vector to clone the 1st LbCas12a crRNA under OsU6 promoter

Depositor

Yiping Qi

Article

Li et al Plant Biotechnol J. 2019 Nov 15. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pICSL1P1_crU626

Plasmid

#191776

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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p33Lb

Plasmid

#158156

Purpose

Expression of crRNA array and LbCas12a using one Pol II promoter. For plant genome editing.

Depositor

Kabin Xie

Article

Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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EC59103

Plasmid

#191785

Purpose

LbCas12a expression in plants

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

AtUbi10::Cas12a+ 8 introns-T-E9

Use

CRISPR

Tags

Expression

Plant

Mutation

D156R

Promoter

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL1P2_crU626

Plasmid

#191777

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL1P7_crU626

Plasmid

#191782

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL1P6_crU626

Plasmid

#191781

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL1P3_crU626

Plasmid

#191778

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL1P4_crU626

Plasmid

#191779

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL1P5_crU626

Plasmid

#191780

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0038

Plasmid

#117605

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for LbCas12a(pEPOR1CB0013) and sgRNA_AtADH1 {AT1G77120}(pEPOR1CB0029)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:LbCas12a(pEPOR1CB0013) ] +[AtU6-26:sgRNA]

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pMAL-his-LbCpf1-EC

Plasmid

#79008

Purpose

Bacterial expression - MBP-his tagged LbCpf1 (E.coli codon optimized)

Depositor

Jin-Soo Kim

Article

Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

Insert

LbCpf1

Use

Tags

MBP-6xHis-NLS

Expression

Bacterial

Mutation

Promoter

tac promoter

Available since

July 1, 2016

Availability

Academic Institutions and Nonprofits only

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pMB30

Plasmid

#122247

Purpose

Lentivirus delivery for stable expression of Lb crRNA, has LbDR; Cloning vector for expression of LbCas12a crRNA. It contains BsmBI site for cloning.

Depositor

Darjus Tschaharganeh

Article

Breinig et al Nat Methods. 2019 Jan;16(1):51-54. doi:

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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pMB34

Plasmid

#122245

Purpose

Lentivirus delivery for stable expression of LbCas12a-VPR

Depositor

Darjus Tschaharganeh

Article

Breinig et al Nat Methods. 2019 Jan;16(1):51-54. doi:

Insert

mammalian codon-optimized Lachnospiraceae bacterium Cas12a fused to VPR activator

Use

Lentiviral

Tags

NLS and VPR activator

Expression

Mutation

Promoter

EFs

Available since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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pTol2-HybridBarcode

Plasmid

#174037

Purpose

For Tol2 transgenesis of expressed barcode containing SpyCas9, SauCas9, and LbCas12a target sites

Depositor

James Gagnon

Article

Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

Insert

CRISPR target sites

Use

Tol2 transgenesis

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pSLQ8454 pHR-hU6-LbCpf1 crRNA BB-EF1a-Puro-WPRE

Plasmid

#140087

Purpose

Backbone for cloning U6 driven LbCpf1 (Cas12a) crRNA, with EF1a driven puromycin resistance

Depositor

Stanley Qi

Article

Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

Insert

LbCpf1 (LbCas12a) crRNA backbone

Use

Lentiviral

Tags

EF1a-Puro

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pSLQ8453 pHR-hU6-LbCpf1 crRNA BB-EF1a-Puro-t2a-BFP-WPRE

Plasmid

#140086

Purpose

Backbone for cloning U6 driven LbCpf1 (Cas12a) crRNA, EF1a driven BFP + puromycin resistance

Depositor

Stanley Qi

Article

Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

Insert

LbCpf1 (LbCas12a) crRNA backbone

Use

Lentiviral

Tags

EF1a-Puro-T2A-BFP

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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LbCpf1-2NLS

Plasmid

#102566

Purpose

bacterial expression of LbCpf1-2NLS

Depositor

Jennifer Doudna

Article

Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

Insert

LbCpf1

Use

Tags

6xHis-MBP-TEV and HA-2xSV40NLS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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