Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 174 results for: adk

Showing: 101 - 120 of 174 results

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pLJC2-Blast-SHMT2-3xFLAG

Plasmid

#233891

Purpose

lentiviral overexpression vector for SHMT2

Depositor

Jason Cantor

Publication

Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

Insert

SHMT2 (SHMT2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR221 NLRP1 A66V

Plasmid

#166817

Purpose

Gateway pDONR vector encoding NLRP1 with the patient mutation A66V (Zhong et al., 2016) (no stop codon)

Depositor

Daniel Bachovchin

Publication

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway Cloning

Mutation

A66V

Available since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 NLRP1 S1213A

Plasmid

#166824

Purpose

Gateway pDONR vector containing NLRP1 with the P1214R patient mutation (no stop codon)

Depositor

Daniel Bachovchin

Publication

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway Cloning

Mutation

S1213A

Available since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac FAK W266A

Plasmid

#186144

Purpose

For expresseion of recombinant FAK using baccolovirus in SF9 cells

Depositor

Michael Rosen

Publication

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PTK2 (PTK2 Mouse)

Tags

His6

Expression

Insect

Mutation

DNA mutation T796G;G797C Amino acid mutation W266A

Promoter

polyhedrin promoter

Available since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221 NLRP1 FIIND (F1079M-L1373) S1213A

Plasmid

#166832

Purpose

Gateway pDONR vector containing NLRP1 FIIND with autoprocessing deficient S1213A mutation (no stop codon)

Depositor

Daniel Bachovchin

Publication

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway Cloning

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pFAST-His-TEV-mEGFP-TEV-FAK-W266A

Plasmid

#258176

Purpose

Used for generation of bacmid DNA and subsequent baculovirus for insect cell expression/purification of FAK-W266A.

Depositor

Lindsay Case

Insert

Ptk2 (Ptk2 Mouse)

Tags

his(6)tev

Expression

Insect

Mutation

Tryptophan 266 is mutated to alanine (W266A) whic…

Promoter

polh

Available since

Aug. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pWZL-hygro-FAKS722A

Plasmid

#216545

Purpose

This retroviral plasmid expresses a mutated cDNA (dominant negative: S722A) of human PTK2

Depositor

Georgia Konstantinidou

Publication

Pozzato et al EMBO Mol Med. 2024 Sep 13. doi: 10.1038/

Insert

PTK2 (S722A) (PTK2 Human)

Use

Retroviral

Tags

No

Expression

Mammalian

Mutation

FAK dominant negative mutation S722A

Available since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pWZL-hygro-FAKY861F

Plasmid

#216546

Purpose

This retroviral plasmid expresses a mutated cDNA (dominant negative: Y861F) of human PTK2

Depositor

Georgia Konstantinidou

Publication

Pozzato et al EMBO Mol Med. 2024 Sep 13. doi: 10.1038/

Insert

PTK2 (Y861F) (PTK2 Human)

Use

Retroviral

Tags

No

Expression

Mammalian

Mutation

FAK dominant negative mutation Y861F

Available since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pWZL-hygro-FAKS732A

Plasmid

#216541

Purpose

This retroviral plasmid expresses a mutated cDNA (dominant negative: S732A) of human PTK2

Depositor

Georgia Konstantinidou

Publication

Pozzato et al EMBO Mol Med. 2024 Sep 13. doi: 10.1038/

Insert

PTK2 (S732A) (PTK2 Human)

Use

Retroviral

Tags

No

Expression

Mammalian

Mutation

FAK dominant negative mutation S732A

Available since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pWZL-hygro-FAKS910A

Plasmid

#216542

Purpose

This retroviral plasmid expresses a mutated cDNA (dominant negative: S910A) of human PTK2

Depositor

Georgia Konstantinidou

Publication

Pozzato et al EMBO Mol Med. 2024 Sep 13. doi: 10.1038/

Insert

PTK2 (S910A) (PTK2 Human)

Use

Retroviral

Tags

No

Expression

Mammalian

Mutation

FAK dominant negative mutation S910A

Available since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pWZL-hygro-FAKP712/713A

Plasmid

#216544

Purpose

This retroviral plasmid expresses a mutated cDNA (dominant negative: P712/713A) of human PTK2

Depositor

Georgia Konstantinidou

Publication

Pozzato et al EMBO Mol Med. 2024 Sep 13. doi: 10.1038/

Insert

PTK2 (P712/713A) (PTK2 Human)

Use

Retroviral

Tags

No

Expression

Mammalian

Mutation

FAK dominant negative mutation P712/713A

Available since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pWZL-hygro-FAKS732A/S910A

Plasmid

#221642

Purpose

This retroviral plasmid expresses a double-mutated cDNA (dominant negative: S732A/S910A) of human PTK2

Depositor

Georgia Konstantinidou

Publication

Pozzato et al EMBO Mol Med. 2024 Sep 13. doi: 10.1038/

Insert

PTK2 (S732A/S910A) (PTK2 Human)

Use

Retroviral

Tags

No

Expression

Mammalian

Mutation

FAK double mutation S732A/S910A (dominant negativ…

Available since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pWZL-hygro-FAKR177/178A

Plasmid

#216543

Purpose

This retroviral plasmid expresses a mutated cDNA (dominant negative: R177/178A) of human PTK2

Depositor

Georgia Konstantinidou

Publication

Pozzato et al EMBO Mol Med. 2024 Sep 13. doi: 10.1038/

Insert

PTK2 (R177/178A) (PTK2 Human)

Use

Retroviral

Tags

No

Expression

Mammalian

Mutation

FAK dominant negative mutation R177/178A

Available since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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PTK2B gRNA (BRDN0001148546)

Plasmid

#76386

Purpose

3rd generation lentiviral gRNA plasmid targeting human PTK2B

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PTK2B (PTK2B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PTK2B gRNA (BRDN0001148298)

Plasmid

#76387

Purpose

3rd generation lentiviral gRNA plasmid targeting human PTK2B

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PTK2B (PTK2B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry WT

Plasmid

#163991

Purpose

Mammalian expression vector encoding codon-optimized human NLRP1 CARD with a C-terminal mCherry tag for imaging

Depositor

Hao Wu

Publication

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NLRP1 CARD (NLRP1 Human)

Tags

mCherry

Expression

Mammalian

Available since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pHBFD1

Plasmid

#53565

Purpose

Produces an active HBFD (carotenoid 4-hydroxy-β-ring 4-dehydrogenase) enzyme in E. coli.

Depositor

Francis X Cunningham Jr

Publication

Cunningham FX et al Plant Cell. 2011 Aug;23(8):3055-69. doi:

Insert

hbfd1 cDNA

Expression

Bacterial

Promoter

lac

Available since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pLJC2-Blast-DHFR-3xFLAG

Plasmid

#233892

Purpose

lentiviral overexpression vector for DHFR

Depositor

Jason Cantor

Publication

Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

Insert

DHFR (DHFR Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR221 NLRP1 P1214R

Plasmid

#166822

Purpose

Gateway pDONR vector encoding NLRP1 with the patient mutation P1214R (Grandemange et al., 2017) (no stop codon)

Depositor

Daniel Bachovchin

Publication

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway Cloning

Mutation

P1214R

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 NLRP1 M77T

Plasmid

#166818

Purpose

Gateway pDONR vector encoding NLRP1 with the patient mutation M77T (Zhong et al., 2016) (no stop codon)

Depositor

Daniel Bachovchin

Publication

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway Cloning

Mutation

M77T

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 101 - 120 of 174 results

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