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pBbS4c-RFP

Plasmid

#35302

Depositor

Jay Keasling

Publication

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

ProE

Available since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pBbE4a-RFP

Plasmid

#35295

Depositor

Jay Keasling

Publication

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

ProE

Available since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pTS1017-Tier1-OTetO7-PCMVmin-SEAP

Plasmid

#169586

Purpose

Tier-1 vector encoding PTetO7-driven SEAP (OTetO7-PCMVmin-SEAP-pA).

Depositor

Martin Fussenegger

Publication

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

tetracycline-controlled SEAP expression

Expression

Mammalian

Promoter

TetO7-PCMVmin

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pPMVAu5 (R)

Plasmid

#98296

Purpose

Enhancing the upper-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters.

Depositor

Claudia Vickers

Publication

Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

Insert

PSSA1-ERG10-TNAT1-PRPL4A-ERG13-TEFM1-PRPL15A-tHMG1-TEBS1

Expression

Yeast

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pVEC1

Plasmid

#241910

Purpose

Primary screening of PET hydrolases and enzyme condition screening. TphR-based terephthalate (TPA) biosensor

Depositor

Neil Dixon

Publication

Alvarez Gonzalez et al Metab Eng Commun. 2024 Nov 6;19:e00250.

Insert

tphR, tphK, sfGFP

Use

Synthetic Biology

Mutation

none

Available since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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NFkBr-IL1Ra

Plasmid

#183065

Purpose

Expresses IL-1Ra downstream of a synthetic promoter activated by the NFkB signaling pathway

Depositor

Farshid Guilak

Publication

Pferdehirt et al Tissue Eng Part A. 2019 May;25(9-10):809

Insert

interleukin-1 receptor antagonist (Il1rn Mouse)

Use

Lentiviral

Available since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pETM6-Pc4CL-CmCHS-MsCHI

Plasmid

#73398

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Publication

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

Pc4CL
CmCHS
MsCHI

Expression

Bacterial

Promoter

T7

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pIVEX-anchor

Plasmid

#46844

Purpose

Template for the amplification of spiking anchors in BeSD

Depositor

Florian Hollfelder

Publication

Diamante et al Protein Eng Des Sel. 2013 Oct;26(10):713

Type

Empty backbone

Use

In vitro expression

Available since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only

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amilCP chromoprotein promoter-less

Plasmid

#118008

Purpose

For course in "Synthetic Biology: A Lab Manual"

Depositor

Anthony Forster

Publication

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

RBS, amilCP

Use

Synthetic Biology; Escherichia coli

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pZ_P-GAP-eGFP

Plasmid

#126717

Purpose

yeast plasmid overexpressing eGFP

Depositor

Menghao Cai

Publication

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

egfp

Expression

Yeast

Promoter

GAP

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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CLYBL_Bxb1-GA_LP_Dual_TC

Plasmid

#229776

Purpose

CLYBL targeting construct with the Bxb1-GA landing pad cassette.

Depositor

Richard Davis

Publication

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Insert

FRT-mScarlet-attP(GA)-PuroR-F3

Use

Synthetic Biology

Expression

Mammalian

Promoter

pGK

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCTCON2-BXG

Plasmid

#158127

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Publication

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

mTagBFP

Expression

Yeast

Promoter

GAL1-10

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET29b_Qcat9

Plasmid

#45137

Depositor

Christopher Petzold

Publication

Batth et al Metab Eng. 2014 Sep 6. pii: S1096-7176(1

Insert

E-coli-pathways-QconCAT9

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pET29b_Qcat10

Plasmid

#45138

Depositor

Christopher Petzold

Publication

Batth et al Metab Eng. 2014 Sep 6. pii: S1096-7176(1

Insert

E-coli-pathways-QconCAT10

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pET29b_Qcat3

Plasmid

#45131

Depositor

Christopher Petzold

Publication

Batth et al Metab Eng. 2014 Sep 6. pii: S1096-7176(1

Insert

E-coli-pathways-QconCAT3

Tags

5XHis

Expression

Bacterial

Promoter

T7

Available since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pIVT-eeBxb1

Plasmid

#232748

Purpose

IVT template for making modRNA encoding eeBxb1

Depositor

Kate Galloway

Publication

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Insert

Bxb1

Use

In vitro transcription

Tags

HA and SV40 NLS

Mutation

V74A, E229K, V375I

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-NG-PE2-BSD

Plasmid

#176933

Purpose

Expresses PE2-NG variant in mammalian cells

Depositor

Hyongbum Kim

Publication

Jang et al Nat Biomed Eng. 2021 Aug 26. pii: 10.103

Insert

PE2-NG variant

Use

Lentiviral

Tags

SV40 NLS

Expression

Mammalian

Promoter

EFS

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Circular-oc-CVB3-GFP

Plasmid

#226262

Purpose

Production of in vitro circularizable RNA via IVT

Depositor

Prashant Mali

Publication

Tong et al Nat Biomed Eng. 2024 Aug 26. doi: 10.103

Insert

EGFP

Use

In vitro transcription

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-Hsp-eGFP-PGK-mCherry

Plasmid

#191862

Purpose

Heat inducible eGFP reporter with constitutive mCherry marker

Depositor

Yingxiao Wang

Publication

Wu et al Nat Biomed Eng. 2021 Nov;5(11):1336-1347

Inserts

eGFP
mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

HSPA7 promoter and PGK promoter

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDL52 (clone45)

Plasmid

#188578

Purpose

High-yield production of coenzyme F420 in E. coli

Depositor

Gerald Lackner

Publication

Last et al Metab Eng. 2022 Jul 18. pii: S1096-7176(

Inserts

ribA
ribD
yigB
fbiC
cofC
cofD
cofI
cofE

Expression

Bacterial

Mutation

Codon optimization, synthetic ribosome binding si…

Promoter

T7 variants

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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