We narrowed to 8,042 results for: sms

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pLU-YFP-ICP0

Plasmid

#134563

Purpose

Expresses YFP-ICP0 fusion protein

Depositor

Susan Janicki

Publication

Newhart et al J Cell Sci. 2012 Nov 15;125(Pt 22):5489-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

YFP-ICP0

Tags

YFP

Expression

Mammalian

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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px459-TDP-43 sgRNA1

Plasmid

#133762

Purpose

Expresses Cas9 and human TDP-43 sgRNA

Depositor

Shawn Ferguson

Publication

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TDP-43 (TARDBP Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pLJM1-FLAG-metap2

Plasmid

#100510

Purpose

Expresses FLAG tagged metap2 in mammalian cells to make lentivirus

Depositor

David Sabatini

Publication

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

METAP2 (METAP2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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STR-KDEL_PGRN-SBP-mCherry

Plasmid

#180439

Purpose

Co-expresses (via IRES) streptavidin-KDEL and Progranulin-SBP-mCherry

Depositor

Shawn Ferguson

Publication

Devireddy et al J Cell Biol. 2022 Feb 7;221(2):e20210404

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Progranulin (GRN Human)

Tags

SBP-mCherry

Expression

Mammalian

Available since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-TDP-43-A382T

Plasmid

#133756

Purpose

Lentiviral expression of human TDP-43 A382T disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Publication

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

Lentiviral

Mutation

Changed alanine 382 to threonine

Promoter

CMV

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Compact mismatched sgRNA library for gene titration (human essential genes, CRISPRi)

Pooled library

#136479

Purpose

Expresses gRNAs for gene titration of ~2400 human genes using CRISPRi

Depositor

Jonathan Weissman

Publication

Jost et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only
GST-RB (561-673)

Plasmid

#31495

Depositor

Julien Sage

Publication

Saddic et al J Biol Chem. 2010 Nov 26. 285(48):37733-

Insert

RB (RB1 Human)

Tags

GST

Expression

Bacterial

Mutation

Contains amino acids 561-673

Available since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
pG2

Plasmid

#162603

Purpose

Edit Ade2 gene in yeast

Depositor

Lars Steinmetz

Publication

Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

Insert

Tef1-Cas9 with RPL25 Intron

Expression

Yeast

Available since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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3XFLAG_GAPDH in pCW57_tTA_Blast

Plasmid

#154211

Purpose

Dox-off lentivirus construct all-in-one vector, with Blast resistance, for expression of GAPDH

Depositor

David Sabatini

Publication

Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

Insert

GAPDH (GAPDH Human)

Use

Lentiviral

Tags

3X FLAG

Expression

Mammalian

Promoter

TRE repeats (dox off)

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pIE2-AD

Plasmid

#21603

Depositor

Takahiro Kusakabe

Publication

Mon et al Anal Biochem. 2009 Sep 15;392(2):180-2.

Insert

NF-kappa-B p65 subunit (Rela Mouse)

Use

Gateway Cloning

Tags

Flag, Gateway destination cassette, and NLS

Expression

Insect

Available since

Sept. 22, 2009

Availability

Academic Institutions and Nonprofits only

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pBS cPitx2 (CT#73)

Plasmid

#13927

Depositor

Cliff Tabin

Publication

Logan et al Cell. 1998 Aug 7. 94(3):307-17.

Insert

pitx2 (PITX2 Chicken)

Expression

Bacterial

Available since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only
pCF525-EF1a-Hygro-P2A-AcrVA5-lenti

Plasmid

#115664

Purpose

Expresses AcrVA5 in human cells, contained in a lentiviral vector.

Depositor

Jennifer Doudna

Publication

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrVA5

Use

Lentiviral

Expression

Bacterial and Mammalian

Promoter

EF-1a promoter

Available since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCF525-EF1a-Hygro-P2A-AcrVA1-lenti

Plasmid

#115662

Purpose

Expresses AcrVA1 in human cells, contained in a lentiviral vector.

Depositor

Jennifer Doudna

Publication

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrVA1

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a promoter

Available since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCsy_complex

Plasmid

#89232

Purpose

Plasmid contains the genes Csy1, Csy2, Csy3, Csy4, cloned as a polycistronic operon from bacterium Pseudomonas aeruginosa PA14. Csy3 includes an N-terminal 6his tag and TEV protease cleavage site

Depositor

Blake Wiedenheft

Publication

Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi:

Inserts

Csy1
Csy2
Csy3
Csy4

Tags

6xHis-TEV protease

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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p8327 pLIX YAP1 S127A

Plasmid

#184528

Purpose

Expression of YAP1 S127A

Depositor

Elizabeth White

Publication

Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

Insert

YAP1 S127A (YAP1 Human)

Use

Lentiviral

Mutation

S127A

Promoter

TRE promoter, Tet ON

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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Zhi3-mCherry

Plasmid

#106295

Purpose

CMV driven mCherry, zeocin resistance

Depositor

Sanford Simon

Publication

Belote et al J Cell Biol. 2020 Jan 6;219(1). pii: 132

Insert

mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DHFR.cHSF1

Plasmid

#134739

Purpose

Lentiviral expression vector for constitutively active HSF1 fused to DHFR destabilized domain

Depositor

Matthew D. Shoulders

Publication

Phillips et al Elife. 2017 Sep 26;6. doi: 10.7554/eLife

Insert

DHFR.cHSF1 (HSF1 Human)

Use

Lentiviral

Tags

DHFR

Expression

Mammalian

Mutation

Deletion of amino acids 186-202

Promoter

CMV

Available since

Aug. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pScaf-1512.1

Plasmid

#111402

Purpose

pScaf phagemid with insert for generating DNA origami scaffold 1512 bases in length

Depositor

Shawn Douglas

Publication

Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

Insert

pScaf-1512

Expression

Bacterial

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-H-RAN-GFP1

Plasmid

#106418

Purpose

H-RAN-GFP1 (RANbody GFP1::Sm-HAtag)

Depositor

Joshua Sanes

Publication

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

H-RAN-GFP1

Tags

HA, His, and Multiple HA

Expression

Mammalian

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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CAG::PSAMQ79G,Q139G:5HT3HC-IRES-GFP

Plasmid

#32474

Purpose

Expresses PSAM-5HT3 HC (Q79G,Q139G) neuronal activator, with IRES-EGFP marker

Depositor

Scott Sternson

Publication

Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PSAMQ79G,Q139G-5HT3 HC

Tags

IRES-EGFP

Expression

Mammalian

Promoter

CAG

Available since

April 10, 2012

Availability

Academic Institutions and Nonprofits only

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