Addgene

We narrowed to 16,496 results for: sacs

Showing: 1201 - 1220 of 16496 results

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p413TEF-Sir2

Plasmid

#51742

Purpose

overexpression of Sir2 in S. cerevisiae, HIS3 marker

Depositor

Markus Ralser

Article

Ralser et al PLoS One. 2009;4(4):e5376. doi: 10.1371/

Insert

Sir2 (SIR2 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

TEF1

Available since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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p413_Csy4NLS

Plasmid

#103139

Purpose

Expresses Csy4-NLS under TEF promoter

Depositor

Jens Nielsen

Article

Ferreira et al ACS Synth Biol. 2017 Nov 21. doi: 10.102

Insert

Csy4

Use

Tags

NLS

Expression

Yeast

Mutation

Promoter

TEF

Available since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pKM263-ScPPX

Plasmid

#38327

Depositor

Florian Freimoser

Article

Werner et al Arch Microbiol. 2005 Nov;184(2):129-36.

Insert

PPX1 (PPX1 Budding Yeast)

Use

Tags

GST, His, and TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pPS1622

Plasmid

#8854

Depositor

Pamela Silver

Article

Prinz et al J Cell Biol. 2000 Aug 7. 150(3):461-74.

Insert

Sec63-sGFP (SEC63 Budding Yeast)

Use

Tags

GFP

Expression

Yeast

Mutation

sGFP=S65T V163A mutant of GFP

Promoter

Available since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
pAMS366-4xCDRE-GFP

Plasmid

#138657

Purpose

stable GFP reporter to monitor calcineurin activity via calcineurin-dependent response element (CDRE) in S. cerevisiae

Depositor

Sabrina Büttner

Article

Diessl et al Microbial Cell, 2020: in press

Insert

4xCDRE-CYC1(PM)-yeGFP

Use

Yeast reporter plasmid

Tags

Expression

Mutation

Promoter

CYC1

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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CelTag Plasmid

Plasmid

#66562

Purpose

Encodes CelTag for the tagging and high affinity purification of endogenous yeast proteins.

Depositor

David Engelke

Article

Carrick et al G3 (Bethesda). 2015 Dec 29. pii: g3.115.

Insert

CelTag

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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ClhN-4V5-APEX2-URA

Plasmid

#207056

Purpose

For ORF tagging with 4V5-APEX2

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

4xV5-APEX2

Use

Tags

4xV5-APEX2

Expression

Yeast

Mutation

Promoter

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET-22b(+)-UTPase

Plasmid

#12667

Depositor

SeWon Suh

Article

Han et al Acta Crystallogr D Biol Crystallogr. 200

Insert

dUTP pyrophosphatase (DUT1 Budding Yeast)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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pCRA03

Plasmid

#103141

Purpose

Triple gRNA expression separated with 28nt Csy4 motif

Depositor

Jens Nielsen

Article

Ferreira et al ACS Synth Biol. 2017 Nov 21. doi: 10.102

Insert

gRNAs

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pMM0531

Plasmid

#128988

Purpose

Part plasmid for VP16-CIB1, Type 3 (coding sequence) part

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

SV40NLS-VP16-CIB1

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0528

Plasmid

#128985

Purpose

Part plasmid for pZF(3BS), Type 2 (promoter) part

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

pZF(3BS)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pTCW002

Plasmid

#83522

Purpose

pheromone mediated destablised-GFP expression in yeast

Depositor

Claudia Vickers

Article

Williams et al ACS Synth Biol. 2013 Mar 15;2(3):136-49.

Insert

pFUS1-yEGFPCLN2PEST

Use

Tags

Expression

Yeast

Mutation

Promoter

pFUS1

Available since

Dec. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR221-Sen1

Plasmid

#99994

Purpose

Shuttle yeast Sen1 into Gateway destination vector

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Sen1 (SEN1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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U6-U57C

Plasmid

#169928

Purpose

WT U6 with a U57C mutation

Depositor

Aaron Hoskins

Article

van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

Insert

U6 U57C

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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p413TEF-HST2

Plasmid

#51745

Purpose

overexpression of HST2 in S. cerevisiae, HIS3 marker

Depositor

Markus Ralser

Article

Ralser et al PLoS One. 2009;4(4):e5376. doi: 10.1371/

Insert

HST2 (HST2 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

TEF1

Available since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pR5-19

Plasmid

#22288

Depositor

Louise Prakash

Article

Pages et al Genetics. 2008 Sep . 180(1):73-82.

Insert

RAD5 (RAD5 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

C914, C917->AA in the C3HC4 ring-finger motif

Promoter

Available since

Nov. 2, 2009

Availability

Academic Institutions and Nonprofits only
pAS2-1-Esf1

Plasmid

#99987

Purpose

Express yeast Esf1 with Y2H binding domain fusion

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Esf1 (ESF1 Budding Yeast)

Use

Tags

Y2H binding domain

Expression

Yeast

Mutation

Promoter

Available since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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YEplac181-GAL11

Plasmid

#8628

Depositor

Jeff Strathern

Article

Malagon et al Genetics 2004 Mar;166(3):1215-27.

Insert

GAL11 (GAL11 Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

Wild type

Promoter

Available since

Nov. 15, 2005

Availability

Academic Institutions and Nonprofits only

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pFC663

Plasmid

#27324

Depositor

Fred Cross

Article

Cross et al Genetics. 2007 Jul . 176(3):1541-55.

Insert

SIC1 (SIC1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Contains insertion relative to SGDB sequence of G…

Promoter

Available since

Feb. 28, 2011

Availability

Academic Institutions and Nonprofits only

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pDB5226

Plasmid

#215493

Purpose

A pAde6 backbone integrating plasmid expressing Pil1-mCherry under the inducible promoter 41nmt1

Depositor

Li-Lin Du

Article

Pan et al Methods Mol Biol. 2025;2862:93-102. doi:

Insert

Pil1-mCherry

Use

Tags

Expression

Yeast

Mutation

Promoter

P41nmt1

Available since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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