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pBiT3.1-C_PRPF4:1-522

Plasmid

#211148

Purpose

Mammalian expression of full-length human PRPF4. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

PRPF4:M1-E522 (PRPF4 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_FBXW10:1-1061

Plasmid

#211207

Purpose

Mammalian expression of full-length human FBXW10 isoform 2. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

FBXW10:M1-I1061 (FBXW10 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

isoform 2. R275L annotated sequence conflict

Promoter

CMV

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pFBD-BirA_PRPF4:1-522

Plasmid

#210896

Purpose

Insect expression of full-length human PRPF4. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

PRPF4:M1-E522 (PRPF4 Human)

Tags

GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBD-BirA_WDR12:90-422

Plasmid

#210893

Purpose

Insect expression of human WDR12 fragment T90 to G422. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR12:T90-G422 (WDR12 Human)

Tags

GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBD-BirA_WDR41:30-459

Plasmid

#210892

Purpose

Insect expression of human WDR41 fragment Q30 to V459. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR41:Q30-V459 (WDR41 Human)

Tags

GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBD-BirA_WDR91:392-747

Plasmid

#210880

Purpose

Insect expression of human WDR91 fragment P392 to A747. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR91:P392-A747 (WDR91 Human)

Tags

GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBD-BirA_WDR91:1-747

Plasmid

#210879

Purpose

Insect expression of full-length human WDR91. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR91:M1-A747 (WDR91 Human)

Tags

GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pBiT3.1-C_WDR25:1-544

Plasmid

#211325

Purpose

Mammalian expression of full-length human WDR25. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR25:M1-H544 (WDR25 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_WDR31:1-367

Plasmid

#211326

Purpose

Mammalian expression of full-length human WDR31 isoform 2. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR31:M1-F367 (WDR31 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

isoform 2. 40-40: Missing

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_WDR33:1-572

Plasmid

#211327

Purpose

Mammalian expression of human WDR33 fragment M1 to I572. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR33:M1-I572 (WDR33 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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