We narrowed to 5,839 results for: pCas

1. pCaST-elav-VP16AD

   Plasmid

   #15308

   Depositor

   Benjamin White

   Article

   Luan et al Neuron. 2006 Nov 9. 52(3):425-36.

   Insert

   VP16AD-ZIP+
   

   Expression

   Insect

   Available Since

   July 6, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAG-Venus-MDGA2

   Plasmid

   #82544

   Purpose

   Overexpression vector for Venus-MDGA2 in neurons

   Depositor

   Alice Ting

   Article

   Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

   Insert

   MDGA2 (Mdga2 Rat)
   

   Tags

   Venus fluorescent protein

   Expression

   Mammalian

   Promoter

   chicken beta actin

   Available Since

   Sept. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pX330-NL-DHFR-SpCas9

   Plasmid

   #124522

   Purpose

   Expresses SpCas9 fused to DHFR domains on both the N-terminus and an internal loop in mammalian cells

   Depositor

   Amit Choudhary

   Article

   Manna et al Angew Chem Int Ed Engl. 2019 May 6;58(19

   Insert

   NL-DHFR-SpCas9
   

   Use

   AAV

   Tags

   Destabilized domain of E.coli dihydrofolate reduc…

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   July 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAG-mDrc7-FLAG

   Plasmid

   #176470

   Purpose

   Expression vector of mouse dynein regulatory complex subunit 7 (Drc7) tagged with FLAG at C-terminus.

   Depositor

   Masahito Ikawa

   Article

   Morohoshi et al PLoS Genet. 2020 Jan 21;16(1):e1008585.

   Insert

   dynein regulatory complex subunit 7 (Drc7 Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CAG promoter

   Available Since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pX330_Actb sgRNA / hSpCas9

   Plasmid

   #172832

   Purpose

   Mammalian expression of a sgRNA targeting the intron 1of Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   sgRNA targeting intron 1 of Actb under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCAG-ChETA-mRuby2-ST

   Plasmid

   #109126

   Purpose

   Cation channelrhodopsin ChETA fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

   Depositor

   Hillel Adesnik

   Article

   Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

   Insert

   ChETA-mRuby2-ST
   

   Expression

   Mammalian

   Available Since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSpCas9(BB)-2A-GFP-G3BP2(1)

   Plasmid

   #136060

   Purpose

   G3BP2 gRNA (#1) inserted into the pSpCas9(BB)-2A-GFP plasmid (TCATACTAAAATTCGTCATG)

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   G3BP2 (G3BP2 Human)
   

   Expression

   Mammalian

   Available Since

   April 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCAG-nDsRedFL-miR9/9*

   Plasmid

   #177805

   Purpose

   The pre-miR-9/9* sequence has been inserted into the EcoRV of pCAG-nDsRedFL-intron.

   Depositor

   Rikako Sanuki

   Article

   Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

   Insert

   miR-9/9*
   

   Expression

   Mammalian

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGL3-B-161;+80pCalb2

   Plasmid

   #66745

   Purpose

   luciferase reporter for Calb2 promoter (-161to +80, WT)

   Depositor

   Emanuela Felley-Bosco, Rolf Stahel

   Article

   Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

   Insert

   Calb2 promoter (-161bp+80) (CALB2 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Promoter

   Calb2

   Available Since

   Jan. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSpCas9n(BB)-2A-puro-a5 guide B

    Plasmid

    #194011

    Purpose

    For CRISPR knockout of integrin alpha5

    Depositor

    Timothy Springer

    Article

    Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

    Insert

    single-guide RNA (a5_guide B)
    

    Use

    CRISPR

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSpCas9n(BB)-2A-puro-aV guide A

    Plasmid

    #194008

    Purpose

    For CRISPR knockout of integrin alphaV

    Depositor

    Timothy Springer

    Article

    Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

    Insert

    single-guide RNA (aV_guide A)
    

    Use

    CRISPR

    Available Since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSpCas9n(BB)-2A-puro-aV guide B

    Plasmid

    #194009

    Purpose

    For CRISPR knockout of integrin alphaV

    Depositor

    Timothy Springer

    Article

    Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

    Insert

    single-guide RNA (aV_guide B)
    

    Use

    CRISPR

    Available Since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSpCas9n(BB)-2A-puro-a5 guide A

    Plasmid

    #194010

    Purpose

    For CRISPR knockout of integrin alpha5

    Depositor

    Timothy Springer

    Article

    Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

    Insert

    single-guide RNA (a5_guide A)
    

    Use

    CRISPR

    Available Since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAG-Ace2_D92N_E199V-mRuby3-ST

    Plasmid

    #129262

    Purpose

    Express soma-localized Ace2_D92N_E199V-mRuby3 in mammalian cells

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

    Insert

    Ace2_D92N_E199V-mRuby3-ST
    

    Expression

    Mammalian

    Mutation

    D92N, E199V

    Promoter

    CAG

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAG-eGtACR1-mRuby2-ST

    Plasmid

    #109136

    Purpose

    Anion channelrhodopsin GtACR1 fused to ER export signal, mRuby2 and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    eGtACR1-mRuby2-ST
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAGGS-Chronos-mRuby2-ST

    Plasmid

    #105446

    Purpose

    Cation channelrhodopsin Chronos fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Pegard et al Nat Commun. 2017 Oct 31;8(1):1228. doi:

    Insert

    Chronos-mRuby2-ST
    

    Tags

    mRuby2 and soma targeting motif

    Promoter

    pCAGGS

    Available Since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCas9-beta-mmr

    Plasmid

    #169241

    Purpose

    Cas9, beta, and mutL mutant expression plasmid for introducing chromosomal point mutations

    Depositor

    Brian Pfleger

    Article

    Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

    Inserts

    cas9
    beta
    mutL-E36K
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Mutation

    Changed glutamic acid 36 to lysine

    Available Since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAS9-mCherry-ACTG1

    Plasmid

    #66943

    Purpose

    CRISPaint target selector ACTG1

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    gRNA ACTG1
    

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG-PxCas12a-2AeGFP

    Plasmid

    #123637

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

    Insert

    PxCas12a
    

    Tags

    3xHA tag

    Expression

    Mammalian

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAGGS-PHFF-sh-mBVRA

    Plasmid

    #100286

    Purpose

    Expression vector for phycocyanobilin (PCB) synthesis and shRNA for mouse BVRA gene in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

    Inserts

    MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
    shRNA for mouse BVRA
    

    Tags

    FLAG, T7 and Mitochondria-targeting sequence (MTS…

    Expression

    Mammalian

    Mutation

    All genes are codon-optimized for expression in h…

    Promoter

    CAG promoter and H1 promoter

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge