We narrowed to 6,459 results for: plasmid dna
-
Plasmid#140984PurposeEncodes a G alpha subunit (GNA15) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlpha15-RLuc8 (GNA15 Human)
UseLuciferaseTagsExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlphai3-RLuc8
Plasmid#140975PurposeEncodes a G alpha subunit (GNAI3) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlphai3-RLuc8 (GNAI3 Human)
UseLuciferaseTagsExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlpha11-RLuc8
Plasmid#140983PurposeEncodes a G alpha subunit (GNA11) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlpha11-RLuc8 (GNA11 Human)
UseLuciferaseTagsExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlphai2-RLuc8
Plasmid#140974PurposeEncodes a G alpha subunit (GNAI2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlphai2-Rluc8 (GNAI2 Human)
UseLuciferaseTagsExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlphaGustducin-RLuc8
Plasmid#140979PurposeEncodes a G alpha subunit (GNAT3) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlphaGustducin-RLuc8 (GNAT3 Human)
UseLuciferaseTagsExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MYO10-HMM-Nanotrap
Plasmid#87255PurposeExpression plasmid to perform NanoSPD 2.0 assays in mammalian cells.DepositorInsertMYO10 (Myo10 Mouse)
UseTagsGFP-nanotrapExpressionMammalianMutationMyo10 is truncated to contain aa.1-941PromoterCMVAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 - HA-KLF4 FL delta K5S
Plasmid#34594DepositorInsertKLF4 (KLF4 Human)
UseTagsHA tagExpressionMammalianMutationdeletion of K5S, corresponding to bases 1781 to 2…PromoterCMVAvailable sinceJan. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/TO-myc-BirA*-cCE
Plasmid#112713PurposeFor tetracycline-inducible expression of myc-BirA*-NES-mCE ΔNLS ("myc-BirA*-cCE") in mammalian cells. For BioID studies.DepositorInsertRngtt (Rngtt Mouse)
UseTagsBirA* (BirA R118G), HIV Rev nuclear localization …ExpressionMammalianMutationSilent mutation at Arg 132 (CGT to CGC), deleted …PromoterCMVAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-LaG16-APT1
Plasmid#234423PurposeExpress APT1 with the anti-GFP nanobody LaG16 fused at the N terminusDepositorInsertAPT1 fused to LaG16 (LYPLA1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-LaG16-APT2
Plasmid#234422PurposeExpress APT2 with the anti-GFP nanobody LaG16 fused at the N terminusDepositorInsertAPT2 fused to LaG16 (LYPLA2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-APT2-LaG16
Plasmid#234421PurposeExpress APT2 with the anti-GFP nanobody LaG16 fused at the C terminus separated by a flexible linkerDepositorInsertAPT2 fused to LaG16 (LYPLA2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-APT1-LaG16
Plasmid#234420PurposeExpress APT1 with the anti-GFP nanobody LaG16 fused at the C terminus separated by a flexible linkerDepositorInsertAPT1 fused to LaG16 (LYPLA1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1751 - pcDNA3.1 CMV-IE V5-ER-LBD
Plasmid#201818PurposeA plasmid expressing the ligand-binding domain of estrogen receptor with a V5 epitope tag from a CMV promoterDepositorInsertV5-ER-LBD
UseTagsExpressionMammalianMutationPromoterCMV-IEAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EFNB2-RBD-Fc (D62Q)
Plasmid#200979PurposeMammalian expression plasmid for soluble EFNB2 (D62Q specificity mutant) fused to IgG1 FcDepositorInsertEFNB2 (EFNB2 Human)
UseTagsIgG1 FcExpressionMammalianMutationSoluble Receptor-Binding Domain; D62Q mutation fo…PromoterCMVAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
RGS-6xHis-LIN-29-pcDNA3.1-
Plasmid#52515Purposeexpresses C. elegans LIN-29 in mammalian cells with RGS-6xHis tag at N-terminusDepositorInsertLin-29 b (lin-29 Nematode)
UseTagsRGS-6xHisExpressionMammalianMutationwt (changed coding sequence in 5' (5th codon…PromoterCMVAvailable sinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
(302) pcDNA3.1(+)-HA-LaG6-(EAAAK)4-OGT(4.5)
Plasmid#168192PurposeHA-tagged GFP nanobody Lag6 (310 nM affinity) fused to a truncated OGT of 4 TPRsDepositorInsertHA-LaG6-(EAAAK)4-OGT(4)
UseTagsHA-TagExpressionMammalianMutationPromoterT7/CMVAvailable sinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Tras heavy chain-GSG-SpyTag003
Plasmid#216292PurposeExpresses anti-HER2 Tras Heavy chain-GSG-SpyTag003 in mammalian cells. To be paired with Tras light chain to form the anti-HER2 Tras FabDepositorInsertTras heavy chain-GSG-SpyTag003
UseAffinity Reagent/ AntibodyTagsHis6 tag, Signal peptide, and SpyTag003ExpressionMammalianMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-C-6His-mEsrra
Plasmid#173152Purposemouse estrogen-related receptor alpha (Esrra) expression plasmid in mammalian cellsDepositorInsertORF clone Estrogen-related receptor alpha (Esrra) (Esrra Mouse)
UseTagsHisExpressionMammalianMutationWild typePromoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 ATP5O(5’UTR)-FF
Plasmid#85486PurposeFirefly luciferase under the control of ATP5O 5'UTRDepositorInsert5'UTR of ATP5O (ATP5PO Human)
UseLuciferaseTagsFirefly luciferaseExpressionMammalianMutationPromoterAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 ATP5O(5’UTR)-SL-FF
Plasmid#85491PurposeFirefly luciferase under the control of ATP5O 5'UTR followed by a stem-loopDepositorInsert5'UTR of ATP5O followed by stem loop (ATP5PO Human)
UseLuciferaseTagsFirefly luciferaseExpressionMammalianMutationPromoterAvailable sinceJan. 13, 2017AvailabilityAcademic Institutions and Nonprofits only