Addgene

We narrowed to 2,673 results for: GUS

Showing: 1261 - 1280 of 2673 results

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Grp1-PH pET11a

Plasmid

#71377

Purpose

Phosphoinositide Binding

Depositor

Mark Lemmon

Article

Ferguson et al Mol Cell. 2000 Aug;6(2):373-84.

Insert

Grp1-PH (Cyth3 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Pleckstrin homology (PH) domain; aa 264-391

Promoter

T7

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pPpT4- TEF2prom-TIR1F74G-FLAG

Plasmid

#189727

Purpose

Enables expression of OsTIR1(F74G) from the TEF2 promoter, used to implement the AID2 system in Komagataella phaffii (original name pLB219)

Depositor

Anita Emmerstorfer-Augustin

Article

Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

Insert

OsTIR1

Use

Tags

FLAG

Expression

Yeast

Mutation

Promoter

TEF2

Available since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pHRep-HKH1

Plasmid

#232909

Purpose

Plasmid for disruption of the phaC1 locus of Cupriavidus necator via homologous recombination

Depositor

Anita Emmerstorfer-Augustin

Article

Arhar et al ACS Synth Biol. 2025 Feb 24. doi: 10.102

Insert

KanR (kanR Synthetic)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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Atg11 [699-800] pHis2

Plasmid

#190264

Purpose

Codon optimized Atg11 amino acids 699-800 from S. cerevisiae for expression in E. coli.

Depositor

Michael Ragusa

Article

Margolis et al J Mol Biol. 2020 Oct 2;432(21):5752-5764

Insert

ATG11

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pPpT4- HTA1prom-TIR1-FLAG

Plasmid

#189725

Purpose

Enables expression of OsTIR1 from the HTA1 promoter, used to implement the AID system in Komagataella phaffii (original name pLLpLL001)

Depositor

Anita Emmerstorfer-Augustin

Article

Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

Insert

OsTIR1

Use

Tags

FLAG

Expression

Yeast

Mutation

Promoter

HTA1

Available since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pPpT4- TEF2prom-TIR1-FLAG

Plasmid

#189726

Purpose

Enables expression of OsTIR1 from the TEF2 promoter, used to implement the AID system in Komagataella phaffii (original name pLL002)

Depositor

Anita Emmerstorfer-Augustin

Article

Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

Insert

OsTIR1

Use

Tags

FLAG

Expression

Yeast

Mutation

Promoter

TEF2

Available since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pACYC_HA-ZmSAE1_ZmSAE2(TRUNC)-FLAG

Plasmid

#91945

Purpose

Bacterial expression of maize SAE1 and truncated SAE2

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Inserts

HA-ZmSAE1
ZmSAE2-FLAG

Use

Tags

FLAG and HA

Expression

Bacterial

Mutation

Truncation of ZmSAE2 to mimic a splice variant

Promoter

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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Ralstonia-GG-Kit

Plasmid Kit

#1000000142

Purpose

Backbone vectors and Level I modules that can be used for assembly of Ralstonia solanacearum species complex gene knockout and complementation constructs based on Golden Gate cloning techniques

Depositor

Thomas Lahaye

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only
pPB-EF1A-Puro-hTBK1-EGFP

Plasmid

#221553

Purpose

Expresses GFP-tagged human TBK1 in mammalian cells. Compatible with piggybac transposase for genome integration.

Depositor

Shawn Ferguson

Article

Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

Insert

TBK1 (TBK1 Human)

Use

Piggybac

Tags

GFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pKESaPar

Plasmid

#211829

Purpose

Low copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.

Depositor

Anita Emmerstorfer-Augustin

Article

Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

Insert

eGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T5

Available since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pKERepPar

Plasmid

#211827

Purpose

Medium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.

Depositor

Anita Emmerstorfer-Augustin

Article

Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

Insert

eGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T5

Available since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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RhGC(BE)_pGEM

Plasmid

#85469

Purpose

The rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signaling

Depositor

Peter Hegemann

Article

Scheib et al Sci Signal. 2015 Aug 11;8(389):rs8. doi:

Insert

rhodopsin-guanylyl cyclase

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7 Promoter

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pQCXIH-PSM-Rb

Plasmid

#37106

Depositor

Joseph Nevins

Article

Angus et al Oncogene. 2012 Jan 16. doi: 10.1038/onc.

Insert

Rb (RB1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

aa 379-928 only; large pocket of human Rb with aa…

Promoter

CMV

Available since

Sept. 21, 2012

Availability

Academic Institutions and Nonprofits only
ALFY-FYVE 1G

Plasmid

#197045

Purpose

Expression plasmid for the FYVE domain from ALFY for recombinant expression in E. coli.

Depositor

Michael Ragusa

Article

Reinhart et al Traffic. 2021 Jan;22(1-2):23-37. doi: 10

Insert

ALFY (WDFY3 Human)

Use

Tags

6xHis-GST-TEV

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 7, 2023

Availability

Academic Institutions and Nonprofits only

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px459-RhebL1 sgRNA

Plasmid

#133769

Purpose

Expresses Cas9 and human RhebL1 sgRNA

Depositor

Shawn Ferguson

Article

Angarola et al Mol Biol Cell. 2019 Oct 15;30(22):2750-2

Insert

RhebL1 sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pPpT4- HTA1prom-TIR1 F74G -FLAG

Plasmid

#189728

Purpose

Enables expression of OsTIR1(F74G) from the HTA1 promoter, used to implement the AID2 system in Komagataella phaffii (original name pLB220)

Depositor

Anita Emmerstorfer-Augustin

Article

Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

Insert

OsTIR1

Use

Tags

FLAG

Expression

Yeast

Mutation

Promoter

HTA1

Available since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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px459-Rheb sgRNA

Plasmid

#133768

Purpose

Expresses Cas9 and human Rheb sgRNA

Depositor

Shawn Ferguson

Article

Angarola et al Mol Biol Cell. 2019 Oct 15;30(22):2750-2

Insert

Rheb sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pAdTrackCMV-PSM-Rb

Plasmid

#37107

Depositor

Joseph Nevins

Article

Angus et al Oncogene. 2012 Jan 16. doi: 10.1038/onc.

Insert

Rb (RB1 Human)

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

aa 379-928 only; large pocket of human Rb with aa…

Promoter

CMV

Available since

May 31, 2012

Availability

Academic Institutions and Nonprofits only
pHInt-AppA-KanR-loxP

Plasmid

#232910

Purpose

Plasmid for marker free integration of E. coli appA at the phac1 locus of Cupriavidus necator.

Depositor

Anita Emmerstorfer-Augustin

Article

Arhar et al ACS Synth Biol. 2025 Feb 24. doi: 10.102

Insert

AppA (appA )

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Pj5

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pRSF_6His-ZmSUMO1a-GG_ZmSCE1d(C94S)-Myc

Plasmid

#91935

Purpose

Bacterial expression of maize SUMO1a and inactive SCE1d

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Inserts

6His-ZmSUMO1a-GG
ZmSCE1d(C94S)-Myc

Use

Tags

6His and Myc

Expression

Bacterial

Mutation

C94S active site mutation and Truncation of ZmSUM…

Promoter

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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