We narrowed to 7,283 results for: ef1a;

1. pLV-EF1a-MinE(L4E)-EGFP_IRES-Blast

   Plasmid

   #213117

   Purpose

   Expression of a faster-running MinE mutant in mammalian cells

   Depositor

   Scott Coyle

   Article

   Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

   Insert

   MinE(L4E)-EGFP (minE Escherichia coli)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   L4E

   Promoter

   EF1a

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. plenti-ef1a-dCasRx-FTO-HA-T2A-BSD

   Plasmid

   #177120

   Purpose

   dCasRx mediated RNA tethering of FTO

   Depositor

   Wenbo Li

   Article

   Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

   Insert

   dCasRx-FTO
   

   Use

   Lentiviral

   Tags

   HA and SV40 NLS

   Expression

   Mutation

   dCasRX contains R239A/H244A/R858A/H863A mutations…

   Promoter

   Ef1a

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHage2-EF1a-TERT K78E-IRES-Puro

   Plasmid

   #182591

   Purpose

   expresses TERT K78E in mammalian cells

   Depositor

   Agnel Sfeir

   Article

   Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

   Insert

   hTERT (TERT Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   K78E

   Promoter

   EF-1a

   Available since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLVX-EF1a-EGFP-Cybt5-IRES-Puromycin

   Plasmid

   #134869

   Purpose

   Expresses EGFP-tagged endoplasmic reticulum marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   cytochrome b5 type A (CYB5A Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-98

   Promoter

   EF1a

   Available since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pX552-EF1a-DIO-mcherry(with gRNA scaffold)

   Plasmid

   #199581

   Purpose

   Expresses mCherry in a Cre-dependent manner

   Depositor

   Bryan Copits

   Article

   Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

   Insert

   mCherry
   

   Use

   AAV, CRISPR, and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a intron A and U6

   Available since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-Ef1a-Con/Foff 2.0-GCaMP6M

   Plasmid

   #137120

   Purpose

   Intersectional viral expression of GCaMP6M in cells expressing Cre AND NOT Flp

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Con/Foff 2.0-GCaMP6M
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   Removed RSET tag

   Promoter

   EF1a

   Available since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLV-EF1a-GFP-SFPQ-IRES-mCherry

   Plasmid

   #166950

   Purpose

   Lentiviral plasmid expressing GFP-tagged SFPQ protein with IRES-mCherry from the EF1a promoter

   Depositor

   Rosalind Segal

   Article

   Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

   Insert

   SFPQ (SFPQ Human)
   

   Use

   Lentiviral

   Tags

   GFP and mCherry

   Expression

   Mutation

   Promoter

   eF1a

   Available since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAVS1_EF1a-GEM2-Halo-FKBP-IRES-Blast

   Plasmid

   #197066

   Purpose

   Donor plasmid for human AAVS1 locus knock-in, constitutive expression of GEM2-Halo-FKBP

   Depositor

   Julia Mahamid

   Article

   Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

   Insert

   Encapsulin
   

   Use

   CRISPR

   Tags

   FKBP and HaloTag

   Expression

   Mammalian

   Mutation

   Codon-optimised for human cell expression

   Promoter

   Available since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CAPTURE2_pLVX-EF1a-NBio-dCas9-IRES-zsGreen1

   Plasmid

   #138419

   Purpose

   CAPTURE2.0 vector containing NBio-dCas9, IRES and zsGreen1

   Depositor

   Jian Xu

   Article

   Liu et al Genome Biol. 2020 Mar 5;21(1):59. doi: 1

   Insert

   dCas9
   

   Use

   Lentiviral

   Tags

   BioTAP-tag

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   March 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCF525-EF1a-Hygro-P2A-AcrVA5-lenti

    Plasmid

    #115664

    Purpose

    Expresses AcrVA5 in human cells, contained in a lentiviral vector.

    Depositor

    Jennifer Doudna

    Article

    Watters et al Science. 2018 Oct 12;362(6411):236-239.

    Insert

    AcrVA5
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF-1a promoter

    Available since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pU6-sgRNA EF1Alpha-puro-T2A-BFP.Control

    Plasmid

    #128749

    Purpose

    Expresses control gRNA

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    Control sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-Ef1a-DIO hChR2(E123A)-EYFP

    Plasmid

    #35507

    Purpose

    AAV expression of EF1a-driven, cre-dependent, hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Insert

    hChR2
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    E123A

    Promoter

    Ef1a

    Available since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-EF1a-DIO-APEX.NES-P2A-EGFP

    Plasmid

    #182824

    Purpose

    Cre-dependent expression of cytosolic APEX

    Depositor

    Yevgenia Kozorovitskiy

    Article

    Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

    Insert

    APEX.NES-P2A-EGFP
    

    Use

    AAV

    Tags

    EGFP reporter (P2A)

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-EF1a-FLEX(frt)-GCaMP6f-WPRE

    Plasmid

    #118273

    Purpose

    pAAV vector for flippase dependent GCaMP6f expression under the control of EF1a promoter

    Depositor

    Naoshige Uchida

    Article

    Menegas et al Nat Neurosci. 2018 Sep 3. pii: 10.1038/s

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    6xHis (N terminal on insert), T7 epitope, and Xpr…

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pOTTC1012 - pAAV EF1a DIO Nuc-eYFP

    Plasmid

    #75082

    Purpose

    An AAV packaging vector that expresses Cre-dependent nuclear-localized eYFP under control of the EF1a promoter.

    Depositor

    Brandon Harvey

    Article

    Sharpe et al Curr Biol. 2017 Jul 24;27(14):2089-2100.

    Insert

    Nuc-eYFP
    

    Use

    AAV and Cre/Lox

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Lenti-EF1alpha-VP64-dCas9-VP64-ZsG

    Plasmid

    #192667

    Purpose

    3rd generation lenti vector encoding VP64-dCas9-VP64 with 2A ZsGreen1

    Depositor

    Jeffrey Miner

    Insert

    VP64-dCas9-VP64, ZsGreen1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9

    Promoter

    EF1a

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLVX-EF1a-EGFP-RAMP4-IRES-Puromycin

    Plasmid

    #134863

    Purpose

    Expresses EGFP-tagged endoplasmic reticulum marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    RAMP4 (SERP1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-Ef1a-Con/Foff 2.0-mCherry

    Plasmid

    #137133

    Purpose

    Intersectional viral expression of mCherry in cells expressing Cre AND NOT Flp

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Foff 2.0-mCherry
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    n/a

    Promoter

    EF1a

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pOttc438 - pAAV EF1a CRE ERT2 bGHpA

    Plasmid

    #210415

    Purpose

    AAV viral vector packaging plasmid that expresses tamoxifen-regulated Cre recombinase

    Depositor

    Christopher Richie, Brandon Harvey

    Insert

    Cre-ERT2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p944-SWITCH-OVER insert: EF1a-Puro

    Plasmid

    #217891

    Purpose

    insert vector for Switch-OVER: Single loxP-STOP-EF1a-Puro-mU6

    Depositor

    Ulrich Elling

    Article

    Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge