We narrowed to 1,735 results for: nif

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pUBC-SiT-EGFP-MS2

Plasmid

#247294

Purpose

Expresses a secretome mRNA reporter: MS2 mRNA encoding 45aa of the Type II membrane protein, sialyltransferase (a Golgi enzyme), fused with EGFP.

Depositor

Jennifer Lippincott-Schwartz

Article

Choi et al Nature. 2025 Nov 5. doi: 10.1038/s41586-

Insert

SiT-EGFP

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

UbC

Available Since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-LAMP1-iLID-Halo

Plasmid

#247296

Purpose

Mammalian expression of LAMP1-iLID-Halo fusion for light-induced recruitment to late endosomes/lysosomes.

Depositor

Jennifer Lippincott-Schwartz

Article

Choi et al Nature. 2025 Nov 5. doi: 10.1038/s41586-

Insert

LAMP1-iLID-Halo

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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ED_p_126

Plasmid

#246073

Purpose

Bacterial expression of ECOR31 2TMbeta residues 72-200 with N-terminal His-SUMO tag and co-expression of ECOR31 mCpol

Depositor

Jennifer Doudna

Article

Doherty et al bioRxiv [Preprint]. 2025 Apr 1:2025.03.2

Insert

2TMbeta

Expression

Bacterial

Available Since

Oct. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pBA1543

Plasmid

#246062

Purpose

Bacterial expression of mutant T4 Acb2 (Y8A) under control of the pJex promoter

Depositor

Jennifer Doudna

Article

Doherty et al bioRxiv [Preprint]. 2025 Apr 1:2025.03.2

Insert

Anti CBASS 2

Expression

Bacterial

Mutation

Y8A

Promoter

pJEX promoter

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pBA1802

Plasmid

#246067

Purpose

Bacterial expression of mutant T4 Acb1 (H44A,H113A) under control of the pJex promoter

Depositor

Jennifer Doudna

Article

Doherty et al bioRxiv [Preprint]. 2025 Apr 1:2025.03.2

Insert

Anti CBASS 1

Expression

Bacterial

Mutation

H44A,H113A

Promoter

pJEX promoter

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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ED_p_060

Plasmid

#246070

Purpose

ECOR31 mCpol bacterial expression under T7 promoter, C-term TEV-MBP-HIS10

Depositor

Jennifer Doudna

Article

Doherty et al bioRxiv [Preprint]. 2025 Apr 1:2025.03.2

Insert

Minimal CRISPR polymerase

Expression

Bacterial

Promoter

T7

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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ED_p_044a

Plasmid

#246071

Purpose

ECOR31 mCpol bacterial expression under T7 promoter, N-term His10-MBP-TEV

Depositor

Jennifer Doudna

Article

Doherty et al bioRxiv [Preprint]. 2025 Apr 1:2025.03.2

Insert

Minimal CRISPR polymerase

Expression

Bacterial

Promoter

T7

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pEM1_Euk_2NLS-WT ( LbCas12a)-2NLS

Plasmid

#244836

Purpose

Mammalian expression of wild-type LbCas12a with 2xSV40 NLS at N-terminus and 2xSV40 at C-terminus

Depositor

Jennifer Doudna

Article

Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

LbCas12a

Use

CRISPR

Tags

2xSV40 NLS

Expression

Mammalian

Promoter

T7

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pEM4_CL7_Bac_2NLS-WT (LbCas12a)-4NLS

Plasmid

#244839

Purpose

Bacterial expression of wild-type LbCas12a with 2xSV40 NLS at N-terminus and 4xSV40 at C-terminus

Depositor

Jennifer Doudna

Article

Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

LbCas12a

Use

CRISPR

Tags

10xHis-CL7-2xSV40 NLS and 4xSV40 NLS

Expression

Bacterial

Promoter

T7

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pEM8_2CTH10_Bac_Lbm6 (LbCas12a)-4NLS

Plasmid

#244843

Purpose

Bacterial expression of Lbm6-LbCas12a with 10xHis-MBP at N-temrinus and 4xSV40 NLS at C-temrinus

Depositor

Jennifer Doudna

Article

Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

Lbm6-Cas12a

Use

CRISPR

Tags

10xHis-MBP and 4xSV40 NLS

Expression

Bacterial

Mutation

D535G/S551F/D665N

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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MCP/PCP yellow RNA Lantern

Plasmid

#226096

Purpose

RNA tracking

Depositor

Jennifer Prescher

Article

Halbers et al Nat Commun. 2024 Nov 18;15(1):9992. doi:

Insert

NLS-HA-MS2-SmBiT-IRES-PP7-Venus d12-LgBiT-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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MCP/PCP red RNA Lantern

Plasmid

#226097

Purpose

RNA tracking

Depositor

Jennifer Prescher

Article

Halbers et al Nat Commun. 2024 Nov 18;15(1):9992. doi:

Insert

NLS-HA-MS2-SmBiT-IRES-PP7-mScarlet-I-LgBiT-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 8, 2025

Availability

Academic Institutions and Nonprofits only

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gRNA_SRR124.5_tagBFP

Plasmid

#163753

Purpose

gRNA expression vector containing the tagBFP fluorescent marker to target the region upstream of the SRR124 SOX2 enhancer in human cells.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

gRNA_SRR124.5

Use

CRISPR

Tags

tagBFP

Expression

Mammalian

Promoter

U6

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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gRNA_SRR134.3_miRFP670

Plasmid

#163754

Purpose

gRNA expression vector containing the miRFP670 fluorescent marker to target the region downstream of the SRR134 SOX2 enhancer in human cells.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

gRNA_SRR134.3

Use

CRISPR

Tags

miRFP670

Expression

Mammalian

Promoter

U6

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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His-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)

Plasmid

#222990

Purpose

Bacterial expression of iGeoCas9(C1) construct

Depositor

Jennifer Doudna

Article

Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

Insert

His-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)

Use

CRISPR

Tags

CL7, His, and MBP

Expression

Bacterial

Promoter

tac

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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JAB2144

Plasmid

#190344

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_6XAmtRO-2XPhlFO::GFPint-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_6XAmtRO-2XPhlFO::GFPint-ADHt

Expression

Plant

Available Since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

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JAB3410

Plasmid

#190410

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proAA::GFPint-ADHter)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_proAA::GFPint-ADHter

Expression

Plant

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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JAB2462

Plasmid

#190369

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_pro35S-LmrAO::GFPint-NLS-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_pro35S-LmrAO::GFPint-NLS-ADHt

Expression

Plant

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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JAB2461

Plasmid

#190368

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_pro35S-IcaRO::GFPint-NLS-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_pro35S-IcaRO::GFPint-NLS-ADHt

Expression

Plant

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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JAB3421

Plasmid

#190411

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_pro35S::GFP-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_pro35S::GFP-ADHt

Expression

Plant

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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