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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,379 results for: ABE8e

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Showing: 121 - 140 of 1379 results

  3. apt-ABE8e

    Plasmid
    #219937
    Purpose
    Mammalian expression of apt-ABE8e protein (Com_93aa-linker_TadA8e) with P2A-EGFP marker
    Depositor
    Alexis Komor
    Publication
    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Com_93-aa_TadA8e
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    219937-plasmid-map-sequence-id-435507

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  5. PCMV-ABE8e-VRER

    Plasmid
    #198018
    Purpose
    A-to-G base editor
    Depositor
    Donald Kohn
    Insert
    ABe8e-VRER
    Expression
    Mammalian
    Available since
    April 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    198018-plasmid-map-sequence-id-390315

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  7. pRN3P_T3_ABE8e_IVT

    Plasmid
    #201676
    Purpose
    Plasmid to be used as DNA template for in vitro RNA transcription of the ABE8e base editor (A to G) by T3 RNA polymerase. The plasmid contains optimised 5'UTR and 3'UTR to improve protein expression.
    Depositor
    Timo Otonkoski
    Publication
    Jalil et al Am J Hum Genet. 2024 Apr 4;111(4):714-72
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ecTadA(8e)-nSpCas9
    Use
    CRISPR; Vector for in-vitro transcription
    Promoter
    T3 promoter
    Available since
    April 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    201676-plasmid-map-sequence-id-406944

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  11. pSCBE3

    Plasmid
    #133966
    Purpose
    Expresses BE3 CRISPR base editor and gRNA scaffold in Streptomyces
    Depositor
    Yuhui Sun
    Publication
    Zhong et al bioRxiv 630137
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BE3
    Use
    CRISPR and Synthetic Biology
    Available since
    Dec. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    133966-plasmid-map-sequence-id-266621

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  12. pSG1039-rA1-SaKKH_P2A_EGFP

    Plasmid
    #239462
    Purpose
    Cytosine base editor with codon-optimized S. aureus KKH Cas9 and rAPOBEC1 deaminase. EGFP can be used as a transfection marker.
    Depositor
    Alexis Komor
    Insert
    S.a. rA1 CBE
    Use
    CRISPR
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    239462-plasmid-map-sequence-id-476829

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  13. pAT15546_nABE8e-SuperFi-Cas9

    Plasmid
    #184376
    Purpose
    Mammalian expression of SuperFi-Cas9 ABE8e base editor
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nABE8e-SuperFi-Cas9
    Use
    CRISPR
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A, Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q10…
    Promoter
    EF-1α core promoter
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    184376-plasmid-map-sequence-id-362137

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  14. pEJS2702-CMV-iNme2-ABE8e-nt

    Plasmid
    #228723
    Purpose
    Expresses N-terminal ABE8e-iNme2 nCas9 (D16A) base editor in mammalian cells
    Depositor
    Erik Sontheimer
    Publication
    Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    N-terminal ABE8e with iNme2 nCas9 (D16A)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Nme2-Cas9 D16A D844G E868K K870R D873A D911G K929…
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    228723-plasmid-map-sequence-id-454010

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  15. pEJS2703-CMV-iNme2Smu-ABE8e-nt

    Plasmid
    #228724
    Purpose
    Expresses N-terminal ABE8e-iNme2Smu nCas9 (D16A) base editor in mammalian cells
    Depositor
    Erik Sontheimer
    Publication
    Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    N-terminal ABE8e with iNme2Smu nCas9 (D16A)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Nme2-Cas9, Smu PID, D16A D844G E868K K870R D873A …
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    228724-plasmid-map-sequence-id-454004

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  18. pSCBE2

    Plasmid
    #133965
    Purpose
    Expresses BE2 CRISPR base editor and gRNA scaffold in Streptomyces
    Depositor
    Yuhui Sun
    Publication
    Zhong et al bioRxiv 630137
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BE2
    Use
    CRISPR and Synthetic Biology
    Available since
    Dec. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    133965-plasmid-map-sequence-id-266618

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  19. pCMV-T7-ABE8.20m-nSpCas9-NG-P2A-EGFP (KAC1164)

    Plasmid
    #185919
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized ABE8.20m-nSpCas9-NG-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185919-plasmid-map-sequence-id-367356

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Showing: 121 - 140 of 1379 results