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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 198 results for: Vibrio

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Showing: 121 - 140 of 198 results

  1. pSL0762 (pQCascade_crRNA-nt)

    Plasmid
    #130636
    Purpose
    Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array encodes a non-targeting guide RNA.
    Depositor
    Samuel H. Sternberg
    Article
    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:
    Insert
    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(non-targeting)
    Use
    CRISPR; Transposon
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    130636-plasmid-map-sequence-id-255017

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  4. pSL0747 (pDonor_L-MmeI)

    Plasmid
    #130647
    Purpose
    Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene. The mutated left end introduces an MmeI recognition site (used for Tn-seq). Total transposon size = 977 bp.
    Depositor
    Samuel H. Sternberg
    Article
    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:
    Insert
    VchCAST donor DNA (L*)
    Use
    CRISPR; Transposon
    Expression
    Bacterial
    Mutation
    TGTTGATG --> TGTTGGAG
    Available Since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    130647-plasmid-map-sequence-id-255647

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  5. pSL0746 (pDonor_R-MmeI)

    Plasmid
    #130646
    Purpose
    Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene. The mutated right end introduces an MmeI recognition site (used for Tn-seq). Total transposon size = 977 bp.
    Depositor
    Samuel H. Sternberg
    Article
    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:
    Insert
    VchCAST donor DNA (R*)
    Use
    CRISPR; Transposon
    Expression
    Bacterial
    Mutation
    TGTTGATA --> TGTTGGAA
    Available Since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    130646-plasmid-map-sequence-id-255642

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  10. pCVD619

    Plasmid
    #66161
    Purpose
    heat-stable enterotoxin from parent strain Vibrio cholera NRT36S
    Depositor
    James Kaper
    Insert
    nag-ST
    Use
    Cloning vector
    Promoter
    Unknown
    Available Since
    July 9, 2015
    Availability
    Academic Institutions and Nonprofits only

  15. pAvA143

    Plasmid
    #248147
    Purpose
    mutated LuxR transcriptional activator for expression in yeast: fused with Gal4 activation domain with 'gen2' mutations
    Depositor
    Emil Jensen
    Article
    van Aalst et al Commun Biol. 2025 Nov 27. doi: 10.1038/s
    Insert
    luxR (gen3 mutations)
    Use
    Integration vector
    Tags
    Gal4 activation domain and nuclear localization s…
    Expression
    Yeast
    Mutation
    Gal4_AD: F51 (TTC→TTT), G88E. LuxR: K53E, N86Y, I…
    Promoter
    pPGK1
    Available Since
    Jan. 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    248147-plasmid-map-sequence-id-497172

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  16. pAvA141

    Plasmid
    #248146
    Purpose
    mutated LuxR transcriptional activator for expression in yeast: fused with Gal4 activation domain with 'gen2' mutations
    Depositor
    Emil Jensen
    Article
    van Aalst et al Commun Biol. 2025 Nov 27. doi: 10.1038/s
    Insert
    luxR (gen2 mutations)
    Use
    Integration vector
    Tags
    Gal4 activation domain and nuclear localization s…
    Expression
    Yeast
    Mutation
    Gal4_AD: G8V. NLS: K120N. LuxR: N86K, K104E, M135V
    Promoter
    pPGK1
    Available Since
    Jan. 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    248146-plasmid-map-sequence-id-497175

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  19. pQCascade-array1

    Plasmid
    #140623
    Purpose
    Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-array1. The crRNA-array1 targets 8 different loci in Escherchia coli.
    Depositor
    Sheng Yang
    Article
    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102
    Insert
    VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-array1
    Use
    CRISPR; Transposon
    Expression
    Bacterial
    Available Since
    July 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    140623-plasmid-map-sequence-id-306003

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  20. pQCascade-IS1

    Plasmid
    #140624
    Purpose
    Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS1. The crRNA-IS1 targets IS1 loci in Escherchia coli.
    Depositor
    Sheng Yang
    Article
    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102
    Insert
    VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS1
    Use
    CRISPR; Transposon
    Expression
    Bacterial
    Available Since
    July 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    140624-plasmid-map-sequence-id-305995

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Showing: 121 - 140 of 198 results