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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,550 results for: golden gate

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Showing: 1401 - 1420 of 6550 results

  1. C1m

    Plasmid
    #120955
    Purpose
    MoClo golden gate assembly CD part for deGFP (deGFP3 with 4aa N-truncation, 9aa C-truncation, F64L, and C203T (numbered with original sequence); see https://www.fpbase.org/protein/degfp3/)
    Depositor
    Richard Murray
    Insert
    deGFP
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120955-plasmid-map-sequence-id-235968

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  2. C21m

    Plasmid
    #120962
    Purpose
    MoClo golden gate assembly CD part for MG-aptamer (fluorescent RNA aptamer of malachite green; see DOI: 10.1021/ja0529788) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    MG-apt
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120962-plasmid-map-sequence-id-238043

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  3. p3m

    Plasmid
    #120935
    Purpose
    MoClo golden gate assembly AB part for J23150 (strength ~400x Anderson promoter; see http://parts.igem.org/Promoters/Catalog/Anderson) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    J23150
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120935-plasmid-map-sequence-id-235944

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  4. p4m

    Plasmid
    #120936
    Purpose
    MoClo golden gate assembly AB part for J23151 (strength ~900x Anderson promoter; see http://parts.igem.org/Promoters/Catalog/Anderson) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    J23151
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120936-plasmid-map-sequence-id-235945

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  5. pAV10.KN

    Plasmid
    #63209
    Purpose
    pCACS backbone (Amp), cloNAT, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).
    Depositor
    Jef Boeke
    Publication
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available since
    April 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    63209-plasmid-map-sequence-id-115799

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  6. C24m

    Plasmid
    #120964
    Purpose
    MoClo golden gate assembly CD part for lambda cI (repressor of pL or pOR1-OR2; use C64m for temperature-sensitive version). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    lambda-cI
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120964-plasmid-map-sequence-id-236100

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  7. C27m

    Plasmid
    #120965
    Purpose
    MoClo golden gate assembly CD part for TetR (tet repressor; identical to C2d but without ssrA degradation tag). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    tetR (no deg tags)
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120965-plasmid-map-sequence-id-236387

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  8. C31m

    Plasmid
    #120966
    Purpose
    MoClo golden gate assembly CD part for Bxb1 integrase. See DOI: 10.1007/s00438-006-0129-5. Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    bxb1 integrase
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120966-plasmid-map-sequence-id-236888

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  9. Rich-pT7GG

    Plasmid
    #108443
    Purpose
    This plasmid is useful for in vitro transcription of genes using T7 promoter. Can be used for Xenopus oocyte assay. It is derived from pT7TS-rich. The vector is compatible for Golden-gate assembly
    Depositor
    Richard Bélanger
    Type
    Empty backbone
    Use
    Xenopus oocyte expression system
    Promoter
    T7
    Available since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    108443-plasmid-map-sequence-id-213670

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  10. pUC-GW-Kan-L0-TaSEP1B6(int)

    Plasmid
    #242006
    Purpose
    L0 CDS1 Golden Gate part of the pre-mRNA (excluding UTRs) of Triticum aestivum SEPALLATA1-6 (SEP1-6) B-subgenome homoeolog (TraesCS5B02G396700)
    Depositor
    Cristobal Uauy
    Insert
    TaSEP1-B6_with_introns
    Use
    Synthetic Biology
    Expression
    Plant
    Available since
    March 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    242006-plasmid-map-sequence-id-504502

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  11. pL0M-SC-RUBY

    Plasmid
    #245485
    Purpose
    Golden Gate Level 0 "SC" RUBY module
    Depositor
    Beth Rowan
    Insert
    RUBY
    Expression
    Plant
    Available since
    Nov. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    245485-plasmid-map-sequence-id-488120

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  12. V35m

    Plasmid
    #121042
    Purpose
    MoClo golden gate assembly AE destination vector DVCK-AE-GFP (ColE1/kan resistance destination vector with constitutive GFP expression for screening.
    Depositor
    Richard Murray
    Insert
    Kan destination vector with ColE1 origin and GFP selection
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121042-plasmid-map-sequence-id-247704

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  13. T1m

    Plasmid
    #121028
    Purpose
    MoClo golden gate assembly DE part for T500 terminator Engineered terminator derived from bacteriophage 83; see DOI: 10.1126/science.284.5414.611).
    Depositor
    Richard Murray
    Insert
    T500_noGap, short attachment (T11)
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121028-plasmid-map-sequence-id-245901

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  14. pAV11K

    Plasmid
    #63184
    Purpose
    pRS416 backbone, URA3 replaced by KanMX, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.
    Depositor
    Jef Boeke
    Publication
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63184-plasmid-map-sequence-id-104886

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  16. pAV10.K5

    Plasmid
    #63210
    Purpose
    pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI or BciVI).
    Depositor
    Jef Boeke
    Publication
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available since
    Feb. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    63210-plasmid-map-sequence-id-104911

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  17. pAV10.K4

    Plasmid
    #63212
    Purpose
    pCACS backbone (Amp), TRP1, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into chrIXR: 387328-388330.
    Depositor
    Jef Boeke
    Publication
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available since
    Feb. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    63212-plasmid-map-sequence-id-104913

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  18. pAV10.F6.loxP

    Plasmid
    #63201
    Purpose
    pCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI).
    Depositor
    Jef Boeke
    Publication
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available since
    Oct. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    63201-plasmid-map-sequence-id-267056

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  19. pAV113.loxP

    Plasmid
    #63186
    Purpose
    pRS413 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.
    Depositor
    Jef Boeke
    Publication
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63186-plasmid-map-sequence-id-131462

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  20. pAV115.loxP

    Plasmid
    #63187
    Purpose
    pRS415 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.
    Depositor
    Jef Boeke
    Publication
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63187-plasmid-map-sequence-id-104891

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Showing: 1401 - 1420 of 6550 results