Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 4,418 results for: Pet2

Showing: 1441 - 1460 of 4418 results

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pET21d-colEdes16/Imdes16

Plasmid

#107124

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des16

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes16/Imdes16

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pET21d-colEdes17/Imdes17

Plasmid

#107125

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des17

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes17/Imdes17

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pET21d-colEdes18/Imdes18

Plasmid

#107126

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des18

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes18/Imdes18

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pET21d-colEdes1/Imdes1

Plasmid

#107108

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des1

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes1/Imdes1

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pET21d-colEdes2/Imdes2

Plasmid

#107109

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des2

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes2/Imdes2

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21d-colEdes3/Imdes3

Plasmid

#107110

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des3

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes3/Imdes3

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21d-colEdes3.5/Imdes3.5

Plasmid

#107111

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des3.5

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes3.5/Imdes3.5

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21d-colEdes4/Imdes4

Plasmid

#107112

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des4

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes4/Imdes4

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21d-colEdes5/Imdes5

Plasmid

#107113

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des5

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes5/Imdes5

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21d-colEdes6/Imdes6

Plasmid

#107114

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des6

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes6/Imdes6

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET29b-clbI S178A

Plasmid

#114159

Purpose

Expresses ClbI S178A

Depositor

Emily Balskus

Article

Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

Insert

clbI

Tags

6xHis and 6xHis-Thrombin site

Expression

Bacterial

Mutation

Mutated S178 to Ala

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-clbH-C-A2-PCP

Plasmid

#114157

Purpose

Expresses ClbH-C-A2-PCP

Depositor

Emily Balskus

Article

Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

Insert

clbH

Tags

6xHis-thrombin-T7 tag

Expression

Bacterial

Mutation

Only contains the C-A2-PCP domains of ClbH

Available Since

Aug. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(P46A)

Plasmid

#112721

Purpose

This mutation (P46A) marks the first residue within the evolved region of U1A.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(P46A)

Tags

6His-TEV

Expression

Bacterial

Mutation

P46A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(R47A)

Plasmid

#112722

Purpose

Arg47 within TBP6.7 is the most critical residue in terms of forming interactions with WT TAR. Mutating this amino acid to Ala results in ~600-fold reduction in Kd.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(R47A)

Tags

6His-TEV

Expression

Bacterial

Mutation

R47A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(R47K)

Plasmid

#112723

Purpose

Arg47 of TBP6.7 interacts with Hoogsteen edge of TAR's Gua26. Mutating this amino acid to Lys reduces binding to the WT TAR RNA ~330-fold.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(R47K)

Tags

6His-TEV

Expression

Bacterial

Mutation

R47K

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(Q48A)

Plasmid

#112724

Purpose

Gln48 contacts the backbone of TAR RNA as well as mediates intramoecular interaction to stabilize the conformation of beta2-beta3 loop within TBP6.7.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(Q48A)

Tags

6His-TEV

Expression

Bacterial

Mutation

Q48A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(Q48T)

Plasmid

#112725

Purpose

This mutant of TBP6.7 binds to TAR very similarly to TBP6.7(WT), with only 1.4-fold reduced Kd.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(Q48T)

Tags

6His-TEV

Expression

Bacterial

Mutation

Q48T

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(T50A)

Plasmid

#112727

Purpose

Lav-evolved amino acid T50 within TBPs is important in engaging intramolecularly to steer also lab-evolved R49 into conformation compatible binding to RNA.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(T50A)

Tags

6His-TEV

Expression

Bacterial

Mutation

T50A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(P51A)

Plasmid

#112728

Purpose

Similar to P46, P51 was selected and is present in all evolved TBPs. Mutating this position into Ala has a modest effect on binding.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(P51A)

Tags

6His-TEV

Expression

Bacterial

Mutation

P51A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(R52A)

Plasmid

#112729

Purpose

52nd position is Arg in both wild-type U1A and TBPs, but the RNA recognition by this residue in both types of proteins is fundamentally different.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(R52A)

Tags

6His-TEV

Expression

Bacterial

Mutation

R52A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

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