We narrowed to 22,613 results for: ckb

1. pUC_sgRNA

   Plasmid

   #68710

   Purpose

   Contains the sgRNA backbone sequence (tracrRNA, 82 bp) and is used as DNA template to amplify a specific sgRNA using a forward primer with the protospacer sequence for gene targeting.

   Depositor

   Roberto Docampo

   Article

   Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 14, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pLKV2-hU6-BbsI-PGK-Puro-TagBFP-WPRE

   Plasmid

   #137994

   Purpose

   Backbone for generation of ClonMapper gRNA barcode library for use in 10X feature barcoding

   Depositor

   Amy Brock

   Article

   Gardner et al Methods Mol Biol. 2022;2394:109-131. doi

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Tol2-krt4-RFP

   Plasmid

   #97149

   Purpose

   As a control of miRNA sponge

   Depositor

   Qing Deng

   Article

   Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

   Type

   Empty backbone

   Use

   Zebrafish

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJUMP44-2A(sfGFP)

   Plasmid

   #126988

   Purpose

   Level 2 vector. sfGFP reporter. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPB-CAG-3xFLAG-empty-pgk-hph

   Plasmid

   #48754

   Purpose

   PiggyBac backbone, CAG promoter, Hygromycin selection, N-terminal 3xFLAG tag

   Depositor

   Austin Smith

   Article

   Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

   Type

   Empty backbone

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   July 19, 2016

   Availability

   Academic Institutions and Nonprofits only

6. mAzami Green-N1 (mAG)

   Plasmid

   #54798

   Purpose

   Localization: N1 Cloning Vector, Excitation: 492, Emission: 505

   Depositor

   Michael Davidson, Atsushi Miyawaki

   Article

   Karasawa et al J Biol Chem. 2003 Sep 5. 278(36):34167-7

   Type

   Empty backbone

   Use

   Tags

   mAzami Green

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 11, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pLenti6.2-ccdB-Renilla

   Plasmid

   #87073

   Purpose

   Lentiviral Gateway-compatible expression vector backbone with a C-terminal Renilla luciferase

   Depositor

   Susan Lindquist, Mikko Taipale

   Article

   Taipale et al Cell. 2012 Aug 31;150(5):987-1001. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Renilla luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mPapaya1-N1

   Plasmid

   #54732

   Purpose

   Localization: N1 Cloning Vector, Excitation: 530, Emission: 541

   Depositor

   Robert Campbell, Michael Davidson

   Article

   Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

   Type

   Empty backbone

   Use

   Tags

   mPapaya1

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFH102

   Plasmid

   #128207

   Purpose

   sgRNA backbone for SaCas9, combine with AtU6-26 promoter module (pFH35)

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   sgRNA backbone for SaCas9, combine with AtU6-26 promoter module (pFH35)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mTFP1-Lifeact-7

    Plasmid

    #54749

    Purpose

    Localization: Actin, Excitation: 462, Emission: 492

    Depositor

    Robert Campbell, Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Type

    Empty backbone

    Use

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pFH103

    Plasmid

    #128208

    Purpose

    crRNA backbone for LbCas12a, combine with AtU6-26 promoter module (pFH35)

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    crRNA backbone for LbCas12a, combine with AtU6-26 promoter module (pFH35)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. mTagRFP-pBAD

    Plasmid

    #54539

    Purpose

    Localization: Protein Expression Vector , Excitation: 555, Emission: 584

    Depositor

    Michael Davidson, Dmitriy Chudakov, Dorus Gadella

    Article

    Merzlyak et al Nat Methods. 2007 Jul;4(7):555-7. Epub 2

    Type

    Empty backbone

    Use

    Tags

    6xHis and mTagRFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pGWB601NL3F10H

    Plasmid

    #141287

    Purpose

    No promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, BASTA R for plant)

    Depositor

    Andrew Millar

    Article

    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

    Type

    Empty backbone

    Use

    Luciferase; Gateway

    Tags

    FLAG (x3), HIS (x10), and NLUC

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAGImC_Empty

    Plasmid

    #92015

    Purpose

    Empty backbone, includes IRES-mCherry

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Type

    Empty backbone

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti6.2-Renilla-ccdB

    Plasmid

    #87076

    Purpose

    Lentiviral Gateway-compatible expression vector backbone with an N-terminal Renilla luciferase

    Depositor

    Susan Lindquist, Mikko Taipale

    Article

    Taipale et al Cell. 2012 Aug 31;150(5):987-1001. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Renilla luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAK005

    Plasmid

    #128218

    Purpose

    improved sgRNA backbone for tRNA-gRNA array, Position 5

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    improved sgRNA backbone for tRNA-gRNA array, Position 5
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAK003

    Plasmid

    #128216

    Purpose

    improved sgRNA backbone for tRNA-gRNA array, Position 3

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    improved sgRNA backbone for tRNA-gRNA array, Position 3
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAK004

    Plasmid

    #128217

    Purpose

    improved sgRNA backbone for tRNA-gRNA array, Position 4

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    improved sgRNA backbone for tRNA-gRNA array, Position 4
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAK006

    Plasmid

    #128219

    Purpose

    improved sgRNA backbone for tRNA-gRNA array, Position 6

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    improved sgRNA backbone for tRNA-gRNA array, Position 6
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEL081

    Plasmid

    #137890

    Purpose

    iSTU-CRISPR/Cas9 with OsCDPK2 intron, tRNA structure backbone

    Depositor

    Yong Zhang

    Article

    Zhong et al Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge