We narrowed to 5,055 results for: cre encoding plasmid

1. GAL4(1-147)

   Plasmid

   #195029

   Purpose

   The control vector GAL4 (1-147) was generated by introducing a stop codon immediately after the GAL4 (1-147) sequence in the GAL4-Creb5 (1-128) vector

   Depositor

   Cheng-Hai Zhang, Andrew Lassar

   Article

   Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

   Insert

   GAL4 (1-147)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFB_ROSA26_SpCas9_gRNAs

   Plasmid

   #174703

   Purpose

   Plasmid containing separate SpCas9 gRNAs for targeted excision of inserted STOP Cassette upstream of reporter alleles found in Ai14 and Ai6 mice. ITRs flank the cassette for easy vector creation.

   Depositor

   Beverly L. Davidson

   Article

   Lang et al Nat Commun. 2019 Jul 30;10(1):3415. doi:

   Insert

   SpCas9 dual gRNAs
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. All_in_one_CRISPR/Cas9_LacZ

   Plasmid

   #74293

   Purpose

   "All-in-one" CRISPR/Cas9 (wt) plasmid for cloning of custom gRNA with blue/white screening

   Depositor

   Lynne Postovit

   Article

   Findlay et al PLoS One. 2016 Apr 18;11(4):e0153901. do

   Inserts

   LacZ-alpha
   Cas9
   mCherry
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Lac promoter, SV40, and T7 promoter

   Available since

   May 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-TRE-GCaMP6s

   Plasmid

   #135418

   Purpose

   Can be used to drive amplified GCaMP6s expression when combined with TET driver . Can be used to create adeno-associated virus to express TET-driven GCaMP6s.

   Depositor

   Rylan Larsen

   Insert

   GCaMP6s
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   Feb. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFA6a-link-tdTomato-SpHis5

   Plasmid

   #44640

   Purpose

   Yeast tagging vector to create gene fusion with tdTomato with SpHis5 selection

   Depositor

   Kurt Thorn

   Insert

   tdTomato
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFA6a-link-mKO1-SpHis5

   Plasmid

   #44642

   Purpose

   Yeast tagging vector to create gene fusion with mKO1 with SpHis5 selection

   Depositor

   Kurt Thorn

   Insert

   mKO1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGGsH6_MCS_SHORT_outFrame

   Plasmid

   #211439

   Purpose

   Vector backbone for random polymerase chain reaction screen

   Depositor

   Helmut Pospiech

   Insert

   MCS-tev cleavage site-EGFP-6xHis-Tag
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDEST27-GST-hSFRP1(aa181-314)

   Plasmid

   #189548

   Purpose

   Protein expression of SFRP1 in mammalian cells. Contains only Netrin (NTR) domain--amino acids 181-314.

   Depositor

   Haian Fu

   Insert

   truncated secreted frizzled related protein 1 (SFRP1 Human)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   contains aa181-314

   Promoter

   Available since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST27-GST-hSFRP1(aa53-169)

   Plasmid

   #189545

   Purpose

   Protein expression of SFRP1 in mammalian cells. Contains only Frizzled cysteine rich domain (CRD)--amino acids 53-169.

   Depositor

   Haian Fu

   Insert

   truncated secreted frizzled related protein 1 (SFRP1 Human)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   contains aa53-169

   Promoter

   Available since

   Oct. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEMS1925

    Plasmid

    #105869

    Purpose

    High copy number plasmid containing iCreERT2 synthesized fragment for constructing the HPRT plasmid

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

    Insert

    iCreERT2
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFA6a-link-mKO1-CaUra3

    Plasmid

    #44638

    Purpose

    Yeast tagging vector to create gene fusion with mKO1 with CaUra3 selection

    Depositor

    Kurt Thorn

    Insert

    mKO1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGGsH6-MCS_SHORT_inFrame

    Plasmid

    #211438

    Purpose

    Vector backbone for random polymerase chain reaction screen

    Depositor

    Helmut Pospiech

    Insert

    MCS-tev cleavage site-EGFP-6xHis-Tag
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSBbi-RP-CoV2/stSpike-puro

    Plasmid

    #161794

    Purpose

    Transposon-based SARS-CoV-2 secretable trimeric Spike stable expression vector for protein production

    Depositor

    Jorge Luis Arias-Arias

    Article

    Arias-Arias et al Biotechnol Rep (Amst). 2023 Mar;37:e0078

    Insert

    SARS-CoV-2 secretable trimeric Spike
    

    Use

    Transposon

    Tags

    6xHis and dTomato

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSc2

    Plasmid

    #80437

    Purpose

    Expresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell

    Depositor

    Ervin Welker

    Article

    Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare

    Inserts

    sp Cas9 gRNA
    eGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    Sept. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-TBG-mem-TurboID

    Plasmid

    #149413

    Purpose

    Hepatocyte-specific expression of secretory pathway membrane-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

    Depositor

    Jonathan Long

    Article

    Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

    Insert

    mem-TurboID
    

    Use

    AAV

    Tags

    HA and V5

    Expression

    Mutation

    Promoter

    TBG

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. XE141 sFRP-1-CS2+

    Plasmid

    #16693

    Depositor

    Randall Moon

    Insert

    sFRP-1 (Sfrp1 Mouse)
    

    Use

    Xenopus expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 22, 2009

    Availability

    Academic Institutions and Nonprofits only

17. MDKA-c009

    Plasmid

    #175463

    Purpose

    Protein expression/secretion in mammalian cells. Entire CDS with ectopic signal peptide, V21-D143

    Depositor

    Opher Gileadi

    Insert

    MDK (MDK Human)
    

    Use

    Tags

    His6 and PTPRS (Gg) SP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEMS1991

    Plasmid

    #49122

    Purpose

    AAV plasmid with FEV (Ple67) promoter driving expression of iCre. Contains WPRE.

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

    Insert

    ssAAV-Ple67-iCre-WPRE
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    FEV

    Available since

    May 6, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pEMS1990

    Plasmid

    #49121

    Purpose

    AAV plasmid with FEV (Ple67) promoter driving expression of iCre.

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

    Insert

    ssAAV-Ple67-iCre
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    FEV

    Available since

    May 6, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pEMS1981

    Plasmid

    #49112

    Purpose

    AAV plasmid with NR2E1 (Ple264) promoter driving expression of iCre.

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

    Insert

    ssAAV-Ple264-iCre
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    NR2E1

    Available since

    May 10, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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