Addgene

We narrowed to 8,429 results for: itch

Showing: 1441 - 1460 of 8429 results

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416 pSG5L HA RB del22

Plasmid

#10721

Depositor

William Sellers

Article

Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

Insert

RB del22 (RB1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Deletion of exon 22 (aa738-775)

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pLX317-SNAI1

Plasmid

#115446

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

SNAI1 (SNAI1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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608 pSG5L HA RB del ex4

Plasmid

#10730

Depositor

William Sellers

Article

Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

Insert

RB del exon4 (RB1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

deletion of exon4

Promoter

Available since

Oct. 7, 2005

Availability

Academic Institutions and Nonprofits only
409 pSG5L HA RB (379-928)

Plasmid

#10734

Depositor

William Sellers

Article

Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

Insert

RB (379-928) (RB1 Human)

Use

Tags

Expression

Mammalian

Mutation

amino acids 379-928

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
498 pSG5L HA RB del663 (NAAIRS)

Plasmid

#10728

Depositor

William Sellers

Article

Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

Insert

RB del663 (RB1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

residues 664 to 669 were replaced by NAAIRS, a fl…

Promoter

Available since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only
Kv7.4 (1-695)/pcDNA3.1

Plasmid

#111453

Purpose

Electrophysiology in HEK cell

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Use

Tags

8xHis and EGFP

Expression

Mammalian

Mutation

Kv7.4 (1-695)

Promoter

Available since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pLX317-QKI

Plasmid

#115441

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

QKI (QKI Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shQKI-3373

Plasmid

#115456

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shQKI-3373 (QKI Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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Kv7.1/pcDNA3.1

Plasmid

#111452

Purpose

Electrophysiology in HEK cell

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 1 isoform 1 [Homo sapiens] (KCNQ1 Human)

Use

Tags

8xHis and EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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Cx36-GCaMP

Plasmid

#123604

Purpose

Mouse Connexin 36-GCaMP3 fusion for measuring gap junction localized calcium signaling - expresses in mammalian cells

Depositor

John O'Brien

Article

Moore et al eNeuro. 2020 Mar 11. pii: ENEURO.0445-19

Insert

Connexin 36-GCaMP3 fusion (Gjd2 Mouse)

Use

Tags

GCaMP3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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pMXs-Ascl1

Plasmid

#134671

Purpose

Retroviral expression of mouse Ascl1

Depositor

Marius Wernig

Article

Tanabe et al Development. 2022 Oct 15;149(20). pii: 2

Insert

Ascl1 (Ascl1 Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pEJS476-pHAGE-TO-Nme dCas9 3XGFP-SgRNA/Telomere-All-in-one

Plasmid

#85716

Purpose

Expresses dNmeCas9-3XsfGFP and telomere-targeting Nme sgRNA

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Inserts

Nme dCas9
telomere sgRNA

Use

CRISPR and Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Mutation

Promoter

CMV-TO and U6

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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alpha catenin conformation sensor

Plasmid

#71709

Purpose

This alpha catenin biosensor undergoes a change in FRET upon mechanical perturbation of the alpha catenin conformation.

Depositor

Deborah Leckband

Article

Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi

Insert

alpha-catenin FRET Sensor

Use

Tags

ECFP and YPET

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3 P-cad

Plasmid

#47502

Purpose

Expresses P-cadherin in mammalian cells

Depositor

Alice Wong

Article

Cheung et al Oncogene. 2010 Apr 22;29(16):2427-40. do

Insert

cadherin 3, type 1, P-cadherin (placental) (CDH3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only

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pEJS477-pHAGE-TO-Spy dCas9 3XmCherry-SgRNA/Telomere-All-in-one

Plasmid

#85717

Purpose

Expresses dSpyCas9-3XmCherry and telomere-targeting Spy sgRNA

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Inserts

Sp dCas9
telomere sgRNA

Use

CRISPR and Lentiviral

Tags

3XmCherry

Expression

Mammalian

Mutation

Promoter

CMV-TO and U6

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pLJM1 TMEM192-Flag

Plasmid

#112742

Purpose

express pLJM1 TMEM192-Flag

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

TMEM192-Flag (TMEM192 Human)

Use

Lentiviral

Tags

Flag

Expression

Mutation

Promoter

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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dmBACCS2-IRES-dOrai-IRES-mCherry

Plasmid

#72894

Purpose

Mammalian expression of dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and mCherry

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

dmBACCS2-IRES-dOrai-IRES-mCherry

Use

Tags

IRES-mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shSNAI1

Plasmid

#115467

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shSNAI1 (SNAI1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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INCTbiosyn-pUB-HspINTphiC31

Plasmid

#127518

Purpose

Plasmid encodes H. sapiens codon optimized Integrase phiC31.

Depositor

Martin Bonamino

Article

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

Integrase phiC31 coding sequence codon optimized for H. sapiens expression.

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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VZV ORF 60 (gL)

Plasmid

#64082

Purpose

eukaryotic expression plasmid for VZV glycoprotein L

Depositor

Randall Cohrs

Article

Birlea et al J Virol. 2013 Jan;87(1):415-21. doi: 10.

Insert

VZV glycoprotein L

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV IE with intron

Available since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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