We narrowed to 5,839 results for: pCas

1. pSAVED-CHAT_PCaspase-C-term

   Plasmid

   #214045

   Purpose

   Bacterial expression plasmid for SAVED-CHAT and PCaspase C-terminal fragment (aa 154-666) from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   SAVED-CHAT-PCaspase
   

   Expression

   Bacterial

   Mutation

   aa 154-666

   Promoter

   lacUV5 promoter

   Available Since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSAVED-CHAT_PCaspase-N-C-term

   Plasmid

   #214046

   Purpose

   Bacterial expression plasmid for SAVED-CHAT, PCaspase N-terminal fragment (aa 1-153), and C-terminal fragment (154-666) from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   SAVED-CHAT-PCaspase
   

   Expression

   Bacterial

   Mutation

   aa 1-153, 154-666

   Promoter

   lacUV5 promoter

   Available Since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSAVED-CHAT-sm_PCaspase

   Plasmid

   #214048

   Purpose

   Bacterial expression plasmid for SAVED-CHAT CHAT-CHAT singlet interface mutant and PCaspase from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   SAVED-CHAT-PCaspase
   

   Expression

   Bacterial

   Mutation

   R456E, D459K, D476K, R479E

   Promoter

   lacUV5 promoter

   Available Since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAG-EGFP-C-IFT46

   Plasmid

   #218724

   Purpose

   Expresses N-terminally EGFP-tagged IFT46 in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

   Insert

   IFT46 (IFT46 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PX458-3xHA-dSpCas9

   Plasmid

   #201953

   Purpose

   Mammalian expression plasmid of dead SpCas9 with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   3xHA-NLS-dSpCas9-T2A-EGFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   D10A and H840A on SpCas9

   Promoter

   Cbh

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pPB-spCas9-pZeo

   Plasmid

   #214121

   Purpose

   Gene expression by Piggybac transposase in mammalian cells

   Depositor

   Sho Yoshimatsu

   Insert

   spCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Codon-optimized for mammalian cells

   Available Since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAGGS-HaloTag-KRas4B(CT)

   Plasmid

   #214276

   Purpose

   Mammalian expression of HaloTag fused to the C-terminal tail of KRas4B

   Depositor

   Shinya Tsukiji

   Article

   Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

   Insert

   HaloTag7-KRas4B(174-188) (KRAS Human)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pX458-tRNA-SpCas9

   Plasmid

   #195183

   Purpose

   Vector for transient Cas9 and EGFP expression. Small tRNA promoter for sgRNA cloning by GoldenGate.

   Depositor

   Stephanie Roessler

   Insert

   Cas9
   

   Use

   CRISPR

   Promoter

   tRNA-Gln

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. eSpCas9(1.1)_NoFlag_ATP1A1_G3_SLBP_G1_Dual_sgRNA

   Plasmid

   #191528

   Purpose

   Vector for tandem expression of SLBP 3'UTR G1 sgRNA in combination with ATP1A1 G3 sgRNA from two independent U6 promoters to facilitate SLBP endogenous tagging by marker free coselection using ouabain

   Depositor

   Yannick Doyon

   Insert

   SLBP 3'UTR G1 sgRNA + ATP1A1 G3 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
   

   Use

   CRISPR; Co-selection via hdr using ouabain

   Expression

   Mammalian

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCaUra3-CUbo-yeGFP

    Plasmid

    #212771

    Purpose

    C-terminal PCR-based tagging with CUbo-yeGFP in budding yeast with CaUra3 selection

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Pcr-based tagging

    Available Since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAG-TSF-ATG101

    Plasmid

    #210858

    Purpose

    Mammalian expression of ATG101

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    ATG101 (ATG101 Human)
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCAGIG-FLAG-SHTN1L

    Plasmid

    #209568

    Purpose

    Mammalian expression of Shtn1 long isoform

    Depositor

    Sika Zheng

    Article

    Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

    Insert

    Shtn1 long isoform (4930506M07Rik Mouse)
    

    Expression

    Mammalian

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAGIG-FLAG-SHTN1S

    Plasmid

    #209569

    Purpose

    Mammalian expression of Shtn1 short isoform

    Depositor

    Sika Zheng

    Article

    Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

    Insert

    Shtn1 short isoform (4930506M07Rik Mouse)
    

    Expression

    Mammalian

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAG/ATP8B4(E170Q)-HA

    Plasmid

    #209243

    Purpose

    Mammalian expression of ATP8B4 mutant E170Q

    Depositor

    Hye-Won Shin

    Article

    Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

    Insert

    ATP8B4 (ATP8B4 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E170Q

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAG/ATP10B(E210Q)-HA

    Plasmid

    #209247

    Purpose

    Mammalian expression of ATP10B mutant E210Q

    Depositor

    Hye-Won Shin

    Article

    Naito et al J Biol Chem. 2015 Jun 12;290(24):15004-1

    Insert

    ATP10B (ATP10B Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E210Q

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAG/ATP8B1(E234Q)-HA

    Plasmid

    #209241

    Purpose

    Mammalian expression of ATP8B1 mutant E234Q

    Depositor

    Hye-Won Shin

    Article

    Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

    Insert

    ATP8B1 (ATP8B1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E234Q

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAG/ATP11B(E180Q)-HA

    Plasmid

    #209250

    Purpose

    Mammalian expression of ATP11B mutant E180Q

    Depositor

    Hye-Won Shin

    Article

    Tone et al FEBS Lett. 2020 Feb;594(3):412-423. doi:

    Insert

    ATP11B (ATP11B Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E180Q

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG/ATP9B(E272Q)-HA

    Plasmid

    #209245

    Purpose

    Mammalian expression of ATP9B mutant E272Q

    Depositor

    Hye-Won Shin

    Article

    Tone et al FEBS Lett. 2020 Feb;594(3):412-423. doi:

    Insert

    ATP9B (ATP9B Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E272Q

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG/ATP10A(E203Q)-HA

    Plasmid

    #209246

    Purpose

    Mammalian expression of ATP10A mutant E203Q

    Depositor

    Hye-Won Shin

    Article

    Naito et al J Biol Chem. 2015 Jun 12;290(24):15004-1

    Insert

    ATP10A (ATP10A Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E203Q

    Available Since

    Nov. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG/ATP8B2(E171Q)-HA

    Plasmid

    #209242

    Purpose

    Mammalian expression of ATP8B2 mutant E171Q

    Depositor

    Hye-Won Shin

    Article

    Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

    Insert

    ATP8B2 (ATP8B2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E171Q

    Available Since

    Nov. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge