We narrowed to 3,222 results for: actin

1. tdTomato-Lifeact-7

   Plasmid

   #54528

   Purpose

   Localization: Actin, Excitation: 554, Emission: 581

   Depositor

   Michael Davidson

   Article

   Fiolka et al Proc Natl Acad Sci U S A. 2012 Apr 3;109

   Type

   Empty backbone

   Use

   Tags

   tdTomato-Lifeact

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. 3xmNeonGreen_UtrCH

   Plasmid

   #129607

   Purpose

   In vivo visualization of F-actin (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   Utrophin
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. LifeAct-MTED

   Plasmid

   #217498

   Purpose

   Expresses LifeAct-mScarlet-MTED for targeting fluorescent, MT depolymerizing peptide MTED to f-actin

   Depositor

   Erik Dent

   Article

   Holland et al Mol Biol Cell. 2024 Jun 1;35(6):mr3. doi

   Insert

   LifeAct-mScarlet-MTED
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pR023_PIE-PUM2

   Plasmid

   #204819

   Purpose

   Expression PIE-PUM2 fusion protein in mammalian cells

   Depositor

   Xiaochang Zhang

   Article

   Ruan et al Nat Commun. 2023 Jun 6;14(1):3275. doi:

   Insert

   Pumilio 2 (PUM2 Human)
   

   Use

   Tags

   HA-Flag-rApobec1 and huADAR2dd

   Expression

   Mammalian

   Mutation

   Promoter

   chicken β-actin promoter

   Available since

   Nov. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-LplA(AILR,36)-NRX3b

   Plasmid

   #43919

   Depositor

   Alice Ting

   Article

   Liu et al PLoS One. 2013;8(2):e52823. doi: 10.1371

   Insert

   AILR-LplA36-NRX3b (lplA Synthetic, Human)
   

   Use

   Tags

   2XHA

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken beta actin

   Available since

   March 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. ITPKA_3xmNeonGreen

   Plasmid

   #129606

   Purpose

   In vivo visualization of F-actin (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   F-tractin
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRRL-VinculinTS-I997A

   Plasmid

   #111832

   Purpose

   Vinculin tension sensor with point mutation affecting ability to bind actin in lentiviral expression vector

   Depositor

   Brenton Hoffman

   Article

   Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

   Insert

   VinculinTS (I997A) (VCL Chicken)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   changed vinculin isoleucine 997 to alanine

   Promoter

   CMV

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-NCad-V

   Plasmid

   #191665

   Purpose

   mVenus-inserted mouse N-cadherin

   Depositor

   Takeharu Nagai

   Article

   Kanadome et al Commun Biol. 2022 Oct 7;5(1):1065. doi:

   Insert

   N-cadherin
   

   Use

   Tags

   HA and mVenus

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken β-actin promoter

   Available since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBlueScript-humanAPOBEC1

   Plasmid

   #134606

   Purpose

   Plasmid bearing APOBEC1 coding sequence. In presence of its cofactor and overexpressing an artificial reporter with editable CAA codon in APOB sequence we can visualize its editing activity

   Depositor

   Silvestro Conticello

   Article

   Chieca et al bioRxiv 2021

   Insert

   humanAPOBEC1 (APOBEC1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Beta actin

   Available since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAG-Cas13d-T2A-Citrine

    Plasmid

    #224565

    Purpose

    Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), Cas13d, and Citrine reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    Cas13d-T2A-Citrine
    

    Use

    CRISPR

    Tags

    T2A-Citrine

    Expression

    Mutation

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. VinculinTS-A50I/I997A

    Plasmid

    #111829

    Purpose

    Vinculin tension sensor with point mutations affecting ability to bind talin and actin

    Depositor

    Brenton Hoffman

    Article

    Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

    Insert

    VinculinTS (I997A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed vinculin alanine 50 to isoleucine and iso…

    Promoter

    CMV

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pOC1 Halo-Actb KI

    Plasmid

    #183426

    Purpose

    CreON knock-in for Halo-beta-actin (amino acid position: before start codon)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and Halo donor
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-Lifeact-Dronpa

    Plasmid

    #182330

    Purpose

    Actin-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.

    Depositor

    Jin Zhang

    Article

    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

    Insert

    Lifeact-Dronpa
    

    Use

    Tags

    N-terminal 17 amino acids from budding yeast Abp1…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEIAV-SIN6.1 CBLacZ-LucWm1A

    Plasmid

    #44167

    Depositor

    John Olsen

    Article

    Patel et al J Gene Med. 2013 Jan;15(1):51-62. doi: 1

    Inserts

    LacZ
    firefly luciferase
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mutation

    Promoter

    CB, CMV enhancer/chicken beta-actin promoter

    Available since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mTFP1-Lifeact-7

    Plasmid

    #54749

    Purpose

    Localization: Actin, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson, Robert Campbell

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Type

    Empty backbone

    Use

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3-LifeAct-Venus

    Plasmid

    #87613

    Purpose

    Actin-targeted Venus (YFP).

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    LifeAct-Venus
    

    Use

    Tags

    17-aa sequence from Abp140 (aka "Lifeact&quo…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAV0782

    Plasmid

    #133514

    Purpose

    Expression of sfGFP tagged LifeAct probe to monitor actin cytoskeleton

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pAde6(PmeI)-p(act1)-LifeAct-sfGFP-terminator(ScADH1)-bsdMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GFP-Rab18

    Plasmid

    #31740

    Depositor

    Ron Vale

    Article

    Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

    Insert

    Rab18 (Rab18 Fly)
    

    Use

    Tags

    GFP

    Expression

    Insect

    Mutation

    Promoter

    Actin

    Available since

    Sept. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pCAGGS-mouse PKA-Calpha-mEGFP

    Plasmid

    #45528

    Depositor

    Haining Zhong

    Article

    Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

    Insert

    PKA catalytic subunit alpha tagged by mEGFP
    

    Use

    With chicken beta actin promoter and wpre

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GFP-Rab6

    Plasmid

    #31734

    Depositor

    Ron Vale

    Article

    Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

    Insert

    Rab6 (Rab6 Fly)
    

    Use

    Tags

    GFP

    Expression

    Insect

    Mutation

    Promoter

    Actin

    Available since

    Sept. 21, 2011

    Availability

    Academic Institutions and Nonprofits only