We narrowed to 15,937 results for: form

1. pSG5 E107K T Ag

   Plasmid

   #133710

   Purpose

   Large T antigen mutant form in pSG5 vector for mammalian expression.

   Depositor

   James A. DeCaprio

   Article

   Zalvide et al Mol Cell Biol. 1995 Oct . 15(10):5800-10

   Insert

   SV40gp6 (SV40gp6 SV40)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E107K

   Promoter

   Available since

   April 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJK370

   Plasmid

   #73595

   Purpose

   Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-2) with C-terminal c-myc epitope

   Depositor

   T. J. Kappock

   Inserts

   delta-12 fatty acid acetylenase
   delta-12 fatty acid desaturase (form 2)
   

   Use

   Tags

   FLAG tag and c-myc tag

   Expression

   Yeast

   Mutation

   Silent mutation in Arg299 codon.

   Promoter

   GAL1 and GAL10

   Available since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS2-Tm2AG

   Plasmid

   #26773

   Depositor

   Shankar Srinivas

   Article

   Trichas et al BMC Biol. 2008 . 6():40.

   Insert

   TdTomato-2A-H2BGFP-SV40pA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mutation in 2A peptide that renders it non-functi…

   Promoter

   Available since

   March 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. CC1215

   Bacterial Strain

   #79062

   Purpose

   BL21(DE3)-based E. coli purine auxotrophic host strain used for the preparation of PurE proteins in a purE background.

   Depositor

   T. J. Kappock

   Bacterial Resistance

   Kanamycin

   Available since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

5. CSH130+pRCP

   Bacterial Strain

   #195860

   Purpose

   WT E. coli strain transformed with plasmid carrying WT chromogenic protein (CP). Serves as control for suppressor strains. Grows purple when CP is induced with rhamnose.

   Depositor

   Gregory Phillips

   Article

   DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)

   Bacterial Resistance

   Chloramphenicol

   Species

   Acropora millepora

   Available since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

6. CSH130+pRCPam

   Bacterial Strain

   #195859

   Purpose

   WT E. coli strain transformed with plasmid carrying chromogenic protein (CP) with amber nonsense mutation in chromophore. Serves as control for suppressor strains. No color when grown with or without rhamnose.

   Depositor

   Gregory Phillips

   Article

   DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)

   Bacterial Resistance

   Chloramphenicol

   Species

   Acropora millepora

   Available since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

7. XA106+pRCPam

   Bacterial Strain

   #195858

   Purpose

   E. coli nonsense suppressor strain (leuX) transformed with plasmid carrying chromogenic protein (CP) with amber nonsense mutation in chromophore. Grows magenta when CP is induced with rhamnose.

   Depositor

   Gregory Phillips

   Article

   DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)

   Bacterial Resistance

   Chloramphenicol

   Species

   Acropora millepora

   Available since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

8. XA103+pRCPam

   Bacterial Strain

   #195857

   Purpose

   E. coli nonsense suppressor strain (tyrT) transformed with plasmid carrying chromogenic protein (CP) with amber nonsense mutation in chromophore. No color when grown with or without rhamnose.

   Depositor

   Gregory Phillips

   Article

   DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)

   Bacterial Resistance

   Chloramphenicol

   Species

   Acropora millepora

   Available since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

9. XA101+pRCPam

   Bacterial Strain

   #195856

   Purpose

   E. coli nonsense suppressor strain (serU) transformed with plasmid carrying chromogenic protein (CP) with amber nonsense mutation in chromophore. Grows salmon when CP is induced with rhamnose.

   Depositor

   Gregory Phillips

   Article

   DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)

   Bacterial Resistance

   Chloramphenicol

   Species

   Acropora millepora

   Available since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

10. XA92+pRCPam

    Bacterial Strain

    #195855

    Purpose

    E. coli nonsense suppressor strain (glnX) transformed with plasmid carrying chromogenic protein (CP) with amber nonsense mutation in chromophore. Grows purple when CP is induced with rhamnose.

    Depositor

    Gregory Phillips

    Article

    DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)

    Bacterial Resistance

    Chloramphenicol

    Species

    Acropora millepora

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

11. pUC18/promEco/Lysostaphin

    Plasmid

    #125765

    Purpose

    Production of staphylolytic protein lysostaphin from a constitutive promoter

    Depositor

    Benedykt Wladyka

    Article

    Madry et al Mol Biotechnol. 2019 Oct;61(10):774-782.

    Insert

    lysostaphin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    sequence corresponding to the mature form protein

    Promoter

    pemIK-Sa1 promoter

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET11a-SUMO3

    Plasmid

    #53143

    Purpose

    E.coli expression of untagged mature human SUMO3 (amino acids 1-93)

    Depositor

    Frauke Melchior

    Article

    Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

    Insert

    SUMO3 (SUMO2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mature form (amino acids 1-93)

    Promoter

    T7

    Available since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET11a-SUMO2

    Plasmid

    #53142

    Purpose

    E.coli expression of untagged mature human SUMO2 (amino acids 1-92)

    Depositor

    Frauke Melchior

    Article

    Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

    Insert

    SUMO2 (SUMO3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mature form (amino acids 1-92)

    Promoter

    T7

    Available since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBABE puro K-Ras V12

    Plasmid

    #9052

    Purpose

    Retroviral expression of a constitutively active form of K-Ras

    Depositor

    William Hahn

    Insert

    KRAS (KRAS Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    G12V. Constitutively active.

    Promoter

    Available since

    Nov. 14, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC18/promSau/Lysostaphin

    Plasmid

    #125766

    Purpose

    Production of staphylolytic protein lysostaphin from a constitutive promoter

    Depositor

    Benedykt Wladyka

    Article

    Madry et al Mol Biotechnol. 2019 Oct;61(10):774-782.

    Insert

    lysostaphin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    sequence corresponding to the mature form protein

    Promoter

    pemIK constitutive promoter

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA-RanWT-mRFP1-polyA

    Plasmid

    #59750

    Purpose

    Express wild-type form of Ran-mRFP1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    Ran-mRFP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Sept. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLTR-SFFV-PTPN2 transcript variant 1-WPRE-UbC-Emerald

    Plasmid

    #201186

    Purpose

    Lentiviral vector plasmid expressing human PTPN2 transcript variant 1 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Bart Nieuwenhuis

    Inserts

    PTPN2, transcript variant 1 (PTPN2 Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDsEndoNA2

    Plasmid

    #174728

    Purpose

    Expresses DsRed-endoNA2 fusion protein for detection of polysialic acid. The endoNA2 is a catalytically inactive form of endosialidase from bacteriophage PK1A2.

    Depositor

    Jukka Finne

    Article

    Lehti et al Nat Commun. 2017 Dec 4;8(1):1915. doi: 1

    Insert

    DsRed-Monomer
    

    Use

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCLXEBR-pTF-kRasV12

    Plasmid

    #114318

    Purpose

    TET-inducible expression of the V12-mutant form of the ki-Ras protein

    Depositor

    Patrick Salmon

    Insert

    Ras (KRAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    G12V oncogenic mutation. Constitutively active K-…

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. CD44Z-c011

    Plasmid

    #175455

    Purpose

    Protein expression/secretion in mammalian cells. CD44s (standard form; exons 2-5, 15-16), A20-E649.

    Depositor

    Opher Gileadi

    Insert

    CD44 (CD44 Human)
    

    Use

    Tags

    His6

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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