We narrowed to 19,742 results for: OMP

1. pAc5.1B-lambdaN-HA-DmHPat_A

   Plasmid

   #145893

   Purpose

   Insect Expression of DmHPat

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmHPat (Patr-1 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   three silent mutations compared to the sequence g…

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. SMT215

   Plasmid

   #134818

   Purpose

   SMT205 with DHFR-FLAG-tag, western blot

   Depositor

   Tanja Kortemme

   Article

   Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1

   Inserts

   Dihydrofolate reductase
   Thymidylate synthase
   

   Use

   Tags

   FLAG

   Expression

   Bacterial

   Mutation

   Silent mutations to eliminate internal restrictio…

   Promoter

   T7 and TET

   Available since

   Oct. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. SMT101

   Plasmid

   #134815

   Purpose

   Dual expression of DHFR and TYMS, in vivo assays

   Depositor

   Tanja Kortemme

   Article

   Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1

   Inserts

   Dihydrofolate reductase
   Thymidylate synthase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Silent mutations to eliminate internal restrictio…

   Promoter

   T7

   Available since

   Oct. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. SMT201

   Plasmid

   #134816

   Purpose

   SMT101 with TET promoter for TYMS, in vivo assays

   Depositor

   Tanja Kortemme

   Article

   Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1

   Inserts

   Dihydrofolate reductase
   Thymidylate synthase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Silent mutations to eliminate internal restrictio…

   Promoter

   T7 and TET

   Available since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAL800

   Plasmid

   #119306

   Purpose

   precusor outer membrane protein A, ompA, with amino acids 1 through 171 deleted and mutations G244C and C290S

   Depositor

   Linda Randall

   Article

   Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

   Insert

   precusor outer membrane protein A, ompA, with amino acids 1 through 171 deleted and mutations G244C and C290S
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   amino acids 1 through 171 deleted, and mutatioms …

   Promoter

   lac

   Available since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHL 990

   Plasmid

   #52992

   Purpose

   pLtetO-1:DsrA, pCon:RhyB, pLlacO-1:rpoS::gfp

   Depositor

   Han Lim

   Article

   Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

   Inserts

   DsrA sRNA
   RhyB sRNA (competitor)
   rpoS
   

   Use

   Synthetic Biology

   Tags

   gfp

   Expression

   Bacterial

   Mutation

   includes −150 to +30 bp relative to start codon

   Promoter

   pCon, pLlacO-1, and pLtetO-1

   Available since

   May 16, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pHL 1035

   Plasmid

   #52999

   Purpose

   pLtetO-1:RhyB, pCon:fhlA::mCherry, pLlacO-1:sodB::gfp

   Depositor

   Han Lim

   Article

   Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

   Inserts

   RhyB sRNA
   fhlA
   sodB
   

   Use

   Synthetic Biology

   Tags

   gfp and mCherry

   Expression

   Bacterial

   Mutation

   contains −309 to +96 bp relative to start codon a…

   Promoter

   pCon, pLlacO-1, and pLtetO-1

   Available since

   May 16, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pHL 1033

   Plasmid

   #52998

   Purpose

   pLtetO-1:RhyB, pCon:rpoS::mCherry, pLlacO-1:sodB::gfp

   Depositor

   Han Lim

   Article

   Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

   Inserts

   RhyB sRNA
   rpoS
   sodB
   

   Use

   Synthetic Biology

   Tags

   gfp and mCherry

   Expression

   Bacterial

   Mutation

   contains −150 and +30 bp relative to start codon …

   Promoter

   pCon, pLlacO-1, and pLtetO-1

   Available since

   May 16, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pHL 1020

   Plasmid

   #52995

   Purpose

   pLtetO-1:RhyB, pCon:DsrA, pLlacO-1:sodB::gfp

   Depositor

   Han Lim

   Article

   Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

   Inserts

   RhyB sRNA
   DsrA sRNA (competitor)
   sodB
   

   Use

   Synthetic Biology

   Tags

   gfp

   Expression

   Bacterial

   Mutation

   contains −56 to +141 bp relative to start codon

   Promoter

   pCon, pLlacO-1, and pLtetO-1

   Available since

   May 16, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pHL 1019

    Plasmid

    #52994

    Purpose

    pLtetO-1:RhyB, pCon:MicC, pLlacO-1:sodB::gfp

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Inserts

    RhyB sRNA
    MicC sRNA (Competitor)
    sodB
    

    Use

    Synthetic Biology

    Tags

    gfp

    Expression

    Bacterial

    Mutation

    contains −56 to +141 bp relative to start codon

    Promoter

    pCon, pLlacO-1, and pLtetO-1

    Available since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pHL 1039

    Plasmid

    #53000

    Purpose

    pLtetO-1:DsrA, pCon:OxyS, pLlacO-1:rpoS::gfp

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Inserts

    DsrA sRNA
    OxyS sRNA (competitor)
    rpoS
    

    Use

    Synthetic Biology

    Tags

    gfp

    Expression

    Bacterial

    Mutation

    contains −150 to +30 bp relative to start codon

    Promoter

    pCon, pLlacO-1, and pLtetO-1

    Available since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pET12a-AL-103 Y32F Y96F

    Plasmid

    #47092

    Depositor

    Marina Ramirez-Alvarado

    Article

    DiCostanzo et al J Biol Chem. 2012 Aug 10;287(33):27997-8

    Insert

    AL-103 Y32F Y96F (IGKV3-20 Human)
    

    Use

    Tags

    ompT

    Expression

    Bacterial

    Mutation

    Y32F Y96F

    Promoter

    T7

    Available since

    Aug. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pCDH_Str-KDEL_ST-SBP-EGFP

    Plasmid

    #65267

    Purpose

    synchronize trafficking of ST from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mutation

    contains only amino acids 1 to 43 of ST

    Promoter

    CMV

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSF3-ultraID

    Plasmid

    #172878

    Purpose

    expresses ultraID in mammalian cells with the tet-on or tet-off system

    Depositor

    Julien Béthune

    Article

    Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

    Insert

    ultraID
    

    Use

    Luciferase and Retroviral; To be used in a tet-on…

    Tags

    myc

    Expression

    Mammalian

    Mutation

    fragment ([aa2-171]) of aquifex aeolicus BirA wit…

    Promoter

    TRE-cmv

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSL1777 (pEffector)

    Plasmid

    #160731

    Purpose

    Effector expression for V. cholerae CAST system, encodes all protein and RNA components. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pCDF backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST proteins and crRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSL0284 (pQCascade_entry)

    Plasmid

    #130635

    Purpose

    Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array contains two BsaI sites for spacer cloning.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(entry)
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSF3-microID

    Plasmid

    #172880

    Purpose

    expresses microID in mammalian cells with the tet-on or tet-off system

    Depositor

    Julien Béthune

    Article

    Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

    Insert

    microID
    

    Use

    Luciferase and Retroviral; To be used in a tet-on…

    Tags

    myc

    Expression

    Mammalian

    Mutation

    fragment ([aa2-171]) of aquifex aeolicus BirA wit…

    Promoter

    TRE-cmv

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HIV-1 LTR-gfp

    Plasmid

    #115809

    Purpose

    HIV-1 LTR driven reporter vector that retains complete LTRs, tat, and rev, but has a frameshift mutation in env, an ngfr reporter gene in place of nef, and gfp in place of gag, pol, vif, and vpr

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    gfp and ngfr
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    HIV1 LTR

    Available since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p221a-GUS

    Plasmid

    #71263

    Purpose

    Entry clone containing the GUS enzyme. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

    Insert

    Beta-glucuronidase
    

    Use

    Gateway

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PatoL-OG241

    Plasmid

    #72841

    Purpose

    Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Bba_B0034 (iGEM). OG241 backbone.

    Depositor

    Chris Barnes

    Article

    Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

    Insert

    PatoL
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PatoL

    Available since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pNLS-2xBFP(4C)

    Plasmid

    #176151

    Purpose

    Encodes NLS-2xBFP(4C), which is a tandem BFP with an N-terminal SV40 NLS. The protein has 4 solvent accessible cysteines for labeling with maleimide dyes.

    Depositor

    Siegfried Musser

    Article

    Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d

    Insert

    NLS-2xBFP(4C)
    

    Use

    Tags

    6xHis tag and SV40 nuclear localization sequence …

    Expression

    Bacterial

    Mutation

    Compared with EGFP, the two BFP domains have the …

    Promoter

    Ptrc

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Str-STIM1-NN_ST-SBP-EGFP

    Plasmid

    #65262

    Purpose

    synchronize trafficking of ST from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Str-STIM1-NN and truncated sialyltransferase (ST6GAL1 Human)
    

    Use

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 43 of ST

    Promoter

    CMV

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pU6-cj-E sgRNA

    Plasmid

    #169915

    Purpose

    Expression of sgRNA on an optimized gRNA scaffold contains A-U pair flip stabilize the CjCas9/sgRNA complex.

    Depositor

    Zhili Rong

    Article

    Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSL0375 (His-MBP-TniQ_Cas8_Cas7_Cas6)

    Plasmid

    #130640

    Purpose

    Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from a T7 promoter, with N-terminal His10-MBP-TEVsite tag on TniQ for purification.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6
    

    Use

    CRISPR; Transposon

    Tags

    His10-MBP-TEVsite on TniQ

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. His-MBP-NRBF2

    Plasmid

    #99330

    Purpose

    Mammalian expression of NRBF2, which associates with the PI3KC3-C1 complex

    Depositor

    James Hurley

    Article

    Young et al Proc Natl Acad Sci U S A. 2016 Jul 19;11

    Insert

    Nuclear receptor-binding factor 2 (NRBF2 Human)
    

    Use

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSL0828 (pQCascade_crRNA-4)

    Plasmid

    #130637

    Purpose

    Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array encodes a guide RNA that targets lacZ.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(target-4)
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSL0762 (pQCascade_crRNA-nt)

    Plasmid

    #130636

    Purpose

    Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array encodes a non-targeting guide RNA.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(non-targeting)
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pNG1256

    Plasmid

    #149710

    Purpose

    Expresses RpoA, RpoB, RpoC-9His and RpoZ from Bacillus subtilis to produce core RNAP complex

    Depositor

    Peter Lewis

    Article

    Newing et al Nat Commun. 2020 Dec 18;11(1):6420. doi:

    Insert

    rpoZ (rpoZ )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. p221z-erVenusYFP

    Plasmid

    #71265

    Purpose

    Entry clone containing ER-localized Venus. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

    Insert

    VenusYFP
    

    Use

    Gateway

    Tags

    ER retention signal HDEL and signal peptide (ER)…

    Expression

    Mutation

    Promoter

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. p221z-erTagRFP

    Plasmid

    #71266

    Purpose

    Entry clone containing ER-localized TagRFP. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

    Insert

    TagRFP
    

    Use

    Gateway

    Tags

    ER retention signal HDEL and signal peptide (ER)…

    Expression

    Mutation

    Promoter

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge