We narrowed to 10,873 results for: CHL

1. pAAV-nEF-Coff/Fon-iC++-EYFP (AAV8)

   Viral Prep

   #137157-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-nEF-Coff/Fon-iC++-EYFP (#137157). In addition to the viral particles, you will also receive purified pAAV-nEF-Coff/Fon-iC++-EYFP plasmid DNA. nEF-driven, Flp-dependent expression of chloride-conducting channelrhodopsin iC++-EYFP (inhibited in presence of Cre) for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Promoter

   nEF

   Tags

   EYFP (Flp-dependent)

   Available since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

2. pAAV-nEF-Con/Foff 2.0-iC++-EYFP (AAV8)

   Viral Prep

   #137156-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-nEF-Con/Foff 2.0-iC++-EYFP (#137156). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Foff 2.0-iC++-EYFP plasmid DNA. nEF-driven, Cre-dependent expression of chloride-conducting channelrhodopsin iC++-EYFP (inhibited in presence of Flp) for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Promoter

   nEF

   Tags

   EYFP (Cre-dependent)

   Available since

   Nov. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

3. psicheck_200_300_CA_repeat

   Plasmid

   #65880

   Purpose

   Luciferase assay vector, assessing regulation of 200-300 nucleotide CA repeats

   Depositor

   Thomas Tuschl

   Insert

   CA repeats 200-300 nucleotides in length
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 2, 2015

   Availability

   Academic Institutions and Nonprofits only

4. psicheck_300_400_CA_repeat

   Plasmid

   #65879

   Purpose

   Luciferase assay vector, assessing regulation of 300-400 nucleotide CA repeats

   Depositor

   Thomas Tuschl

   Insert

   CA repeats 300-400 nucleotides in length
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 2, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pFRT-TODestFLAGHAhTTP

   Plasmid

   #48738

   Purpose

   Expresses hTTP

   Depositor

   Thomas Tuschl

   Insert

   TTP (ZFP36 Human)
   

   Use

   Tags

   FLAG, HA, and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, 2x Tet

   Available since

   June 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR221_UBE2V1_3UTR

   Plasmid

   #65864

   Purpose

   Carries 3' UTR of UBE2V1, an RBPMS target

   Depositor

   Thomas Tuschl

   Insert

   UBE2V1 (UBE2V1 Human)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   June 25, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pUPD2 NtSPrbcS (GB2213)

   Plasmid

   #160589

   Purpose

   B3 for signal peptide (transit peptide to chloroplast) from Ribulose bisphosphate carboxylase small chain

   Depositor

   Diego Orzaez

   Insert

   NtSPrbcS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR221_NDUFA6_3UTR

   Plasmid

   #65863

   Purpose

   Carries 3' UTR of NDUFA6, an RBPMS target

   Depositor

   Thomas Tuschl

   Insert

   NDUFA6 (NDUFA6 Human)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   July 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC19-link-mCherry-FLAG:cat

   Plasmid

   #211831

   Purpose

   Plasmid template that encodes the fluorescent protein mCherry with a FLAG tag next to chloramphenicol resistence gene. This template was used to copy out mcherry-FLAG-cat for genetic tagging.

   Depositor

   Peng Chen

   Insert

   mCherry-FLAG-cat
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   NA

   Promoter

   Available since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pINIT_cat

    Plasmid

    #46858

    Purpose

    FX sequencing vector for subcloning, chloramphenicol marker

    Depositor

    Eric Geertsma, Raimund Dutzler

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Use

    Sequencing vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC19-link-PAmCherry-FLAG:cat

    Plasmid

    #211832

    Purpose

    Plasmid template that encodes the fluorescent protein PAmCherry1 with a FLAG tag next to chloramphenicol resistence gene. This template was used to copy out PAmcherry1-FLAG-cat for genetic tagging.

    Depositor

    Peng Chen

    Insert

    PAmCherry-FLAG-cat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    NA

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. Ef1a-somBiPOLES-mCerulean-IRES-Puro

    Plasmid

    #192585

    Purpose

    lentiviral vector for bidirectional optogenetic manipulation of Cre-positive neurons

    Depositor

    Simon Wiegert

    Insert

    somBiPOLES
    

    Use

    Lentiviral

    Tags

    soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1-alpha

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. psicheck_NDUFA6_3UTR

    Plasmid

    #65886

    Purpose

    Luciferase assay vector, assessing regulation of NDUFA6 3' UTR

    Depositor

    Thomas Tuschl

    Insert

    NDUFA6 (NDUFA6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2015

    Availability

    Academic Institutions and Nonprofits only

14. TRE3GV-somBiPOLES-mKate2

    Plasmid

    #192579

    Purpose

    AAV-vector for bidirectional optogenetic manipulation of tTA-positive neurons

    Depositor

    Simon Wiegert

    Insert

    somBiPOLES
    

    Use

    AAV

    Tags

    soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3GV

    Available since

    Dec. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. TRE3GV-somBiPOLES-mCerulean

    Plasmid

    #192578

    Purpose

    AAV-vector for bidirectional optogenetic manipulation of tetTag-positive neurons

    Depositor

    Simon Wiegert

    Insert

    somBiPOLES
    

    Use

    AAV

    Tags

    soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3GV

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. CaMKII-somBiPOLES-eGFP

    Plasmid

    #192583

    Purpose

    AAV-vector for bidirectional optogenetic manipulation of excitatory neurons

    Depositor

    Simon Wiegert

    Insert

    somBiPOLES
    

    Use

    AAV

    Tags

    soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa(0.4)

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. PHB1

    Plasmid

    #212153

    Purpose

    For PHB production in E. coli (PT7-pMB1).

    Depositor

    Kang Zhou

    Insert

    poly(3-hydroxyalkanoate) polymerase subunit PhaC, PHA-specific beta-ketothiolase, acetoacetyl-CoA reductase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PT7

    Available since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. hSyn-DIO-somBiPOLES-eGFP

    Plasmid

    #192584

    Purpose

    AAV-vector for bidirectional optogenetic manipulation of Cre-positive neurons

    Depositor

    Simon Wiegert

    Insert

    somBiPOLES
    

    Use

    AAV

    Tags

    soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. 25 pCOLI_S_NoTag

    Plasmid

    #160261

    Purpose

    Untagged Spec resistant bacterial expression vector

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 41 pCOLI_K_NoTag

    Plasmid

    #160265

    Purpose

    Untagged Kan resistant bacterial expression vector

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge