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Showing: 1521 - 1540 of 2789 results

  5. SP6-sgRNA-scaffold

    Plasmid
    #47912
    Purpose
    Scaffold into which clone a 20 bp targeting sequence to generate a plasmid for in vitro transcription of an sgRNA using SP6 RNA polymerase for use in CRISPR-Cas by RNA injection
    Depositor
    Paul Sternberg
    Article
    Chiu et al Genetics. 2013 Aug 26.
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    sgRNA 3' end
    Expression
    Worm
    Mutation
    Promoter
    SP6
    Available since
    Sept. 13, 2013
    Availability
    Academic Institutions and Nonprofits only
    47912-plasmid-map-sequence-id-68534

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  10. pMB66

    Plasmid
    #47946
    Purpose
    Expresses C. elegans optimized Cas9::mEGFP from the hsp-16.48 promoter
    Depositor
    Mike Boxem
    Article
    Waaijers et al Genetics. 2013 Aug 26.
    Insert
    Cas9
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, egl-13 NLS, and mEGFP
    Expression
    Worm
    Mutation
    Promoter
    hsp-16.48
    Available since
    Dec. 6, 2013
    Availability
    Academic Institutions and Nonprofits only
    47946-plasmid-map-sequence-id-68806

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  12. XW52

    Plasmid
    #65838
    Purpose
    XW52 Pmig-13-Czipper-QF-AD-NLS-SL2-mCherry-3'UTR-unc-54 for Split Q intersectional method
    Depositor
    Kang Shen
    Article
    Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi
    Insert
    Czipper-QF-AD-NLS
    Use
    Tags
    mCherry
    Expression
    Worm
    Mutation
    Promoter
    Pmig-13
    Available since
    Oct. 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    65838-plasmid-map-sequence-id-120568

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  17. pSA120

    Plasmid
    #59789
    Purpose
    C. elegans heat-shock driven ZIF-1 and mCherry co-expressed in the same operon
    Depositor
    Jeremy Nance
    Article
    Armenti et al Development. 2014 Dec;141(23):4640-7. do
    Inserts
    hsp-16.41 promoter
    zif-1 coding
    gpd-2/gpd-3 intergenic sequence
    mCherry (worm)
    unc-54 3' UTR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    59789-plasmid-map-sequence-id-95470

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  18. pMB62

    Plasmid
    #47944
    Purpose
    Expresses C. elegans optimized Cas9::mEGFP from the eef-1A.1 (eft-3) promoter
    Depositor
    Mike Boxem
    Article
    Waaijers et al Genetics. 2013 Aug 26.
    Insert
    Cas9
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, egl-13 NLS, and mEGFP
    Expression
    Worm
    Mutation
    Promoter
    eef-1A.1 (eft-3)
    Available since
    Oct. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    47944-plasmid-map-sequence-id-68804

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Showing: 1521 - 1540 of 2789 results