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Plasmid#232980PurposeGalactose iduced expression of Gcn4 WTSLFD+ in yeastDepositorInsertGcn4 WTSLFD+
UseTags3xHA and TEV cleavage siteExpressionYeastMutationF108W, E109T, Y110S, E111L, N112F, L113DPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Aro2+
Plasmid#232982PurposeGalactose iduced expression of Gcn4 Aro2+ in yeastDepositorInsertGcn4 Aro2+
UseTagsTEV cleavage siteExpressionYeastMutationN116F, S117E, E119N, P129Q, T132YPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Aro3+
Plasmid#232983PurposeGalactose iduced expression of Gcn4 Aro3+ in yeastDepositorInsertGcn4 Aro3+
UseTagsTEV cleavage siteExpressionYeastMutationD103Y, N112T, L113Y, S117F, K140RPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 W120AHA
Plasmid#232992PurposeGalactose iduced expression of Gcn4 W120AHA in yeastDepositorInsertGcn4 W120A
UseTags3xHA and TEV cleavage siteExpressionYeastMutationW120APromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Aro3+HA
Plasmid#232990PurposeGalactose iduced expression of Gcn4 Aro3+HA in yeastDepositorInsertGcn4 Aro3+
UseTags3xHA and TEV cleavage siteExpressionYeastMutationD103Y, N112T, L113Y, S117F, K140RPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Aro2+HA
Plasmid#232989PurposeGalactose iduced expression of Gcn4 Aro2+HA in yeastDepositorInsertGcn4 Aro2+
UseTags3xHA and TEV cleavage siteExpressionYeastMutationN116F, S117E, E119N, P129Q, T132YPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 F124IGFP
Plasmid#233005PurposeGalactose iduced expression of Gcn4 F124IGFP in yeastDepositorInsertGcn4 F124I
UseTagsHA-GFP-HA and TEV cleavage siteExpressionYeastMutationF124IPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 WW+HA
Plasmid#232991PurposeGalactose iduced expression of Gcn4 WW+HA in yeastDepositorInsertGcn4 WW+
UseTags3xHA and TEV cleavage siteExpressionYeastMutationE109N, S117D, T121W, N126W, T132WPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 WW+GFP
Plasmid#233003PurposeGalactose iduced expression of Gcn4 WW+GFP in yeastDepositorInsertGcn4 WW+
UseTagsHA-GFP-HA and TEV cleavage siteExpressionYeastMutationE109N, S117D, T121W, N126W, T132WPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 W120AGFP
Plasmid#233004PurposeGalactose iduced expression of Gcn4 120AGFP in yeastDepositorInsertGcn4 W120A
UseTagsHA-GFP-HA and TEV cleavage siteExpressionYeastMutationW120APromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Aro2+GFP
Plasmid#233001PurposeGalactose iduced expression of Gcn4 Aro2+GFP in yeastDepositorInsertGcn4 Aro2+
UseTagsHA-GFP-HA and TEV cleavage siteExpressionYeastMutationN116F, S117E, E119N, P129Q, T132YPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 S/ArotoL
Plasmid#232979PurposeGalactose iduced expression of Gcn4 S/ArotoL in yeastDepositorInsertGcn4 S/ArotoL
UseTagsTEV cleavage siteExpressionYeastMutationS101L, S104L, F108L, Y110L, S117L, S136L, S144LPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 P+
Plasmid#232971PurposeGalactose iduced expression of Gcn4 Proline in yeastDepositorInsertGcn4 Proline
UseTagsTEV cleavage siteExpressionYeastMutationS122P, T131F, A141P, E143DPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 9acidA
Plasmid#232967PurposeGalactose iduced expression of Gcn4 9acidA in yeastDepositorInsertGcn4 9acidA
UseTagsTEV cleavage siteExpressionYeastMutationE109A, E111A, E114A, D115A, E119A, D125A, D127A, …PromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 E2K2
Plasmid#232968PurposeGalactose iduced expression of Gcn4 EK+ in yeastDepositorInsertGcn4 EK+
UseTagsTEV cleavage siteExpressionYeastMutationS104K, S117E, S136E, S144KPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
ppAG415Gal_Gcn4 L113A+
Plasmid#232972PurposeGalactose iduced expression of Gcn4 L113A in yeastDepositorInsertGcn4 L113A
UseTagsTEV cleavage siteExpressionYeastMutationL113A, E114Y, N126H, D127E, S136APromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Aro3+GFP
Plasmid#233002PurposeGalactose iduced expression of Gcn4 Aro3+GFP in yeastDepositorInsertGcn4 Aro3+
UseTagsHA-GFP-HA and TEV cleavage siteExpressionYeastMutationD103Y, N112T, L113Y, S117F, K140RPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 ILVtoED
Plasmid#232957PurposeGalactose iduced expression of Gcn4 ILVtoED in yeastDepositorInsertGcn4 ILVtoED
UseTagsTEV cleavage siteExpressionYeastMutationL123D, I128E, V130E, V135E, L137D, I142DPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 StoL
Plasmid#232981PurposeGalactose iduced expression of Gcn4 StoL in yeastDepositorInsertGcn4 StoL
UseTagsTEV cleavage siteExpressionYeastMutationS104L, S117L, S136L, S144LPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 R3W
Plasmid#232975PurposeGalactose iduced expression of Gcn4 R++ W+ in yeastDepositorInsertGcn4 R++ W+
UseTags3xHA and TEV cleavage siteExpressionYeastMutationD103R, N126W, D133R, K143RPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 E3HA
Plasmid#232996PurposeGalactose iduced expression of Gcn4 E+HA in yeastDepositorInsertGcn4 E+
UseTags3xHA and TEV cleavage siteExpressionYeastMutationD105E, D118E, D139EPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 F108A+
Plasmid#232974PurposeGalactose iduced expression of Gcn4 F108A in yeastDepositorInsertGcn4 F108A
UseTagsTEV cleavage siteExpressionYeastMutationF108A, T121L, A141PPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 ILVtoAS
Plasmid#232959PurposeGalactose iduced expression of Gcn4 ILVtoAS in yeastDepositorInsertGcn4 ILVtoAS
UseTagsTEV cleavage siteExpressionYeastMutationL123S, I128S, V130A, V135A, L137S, I142SPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 S/ArotoL
Plasmid#231880PurposeBacterial expression of N-terminally 6His tagged Gcn4 SArotoLDepositorInsertGcn4 S/ArotoL
UseTags6xHis, TEV cleavage siteExpressionBacterialMutationS101L, S104L, F108L, Y110L, S117L, S136L, S144LPromoterT7Available sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 solvvol W
Plasmid#231878PurposeBacterial expression of N-terminally 6His tagged Gcn4 solvvol WDepositorInsertGcn4 solvvol W
UseTags6xHis, TEV cleavage siteExpressionBacterialMutationS101T, D103E, S104T, D115E, N116Q, S117T, S122T, …PromoterT7Available sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 solvvol
Plasmid#231877PurposeBacterial expression of N-terminally 6His tagged Gcn4 solvvolDepositorInsertGcn4 solvvol
UseTags6xHis, TEV cleavage siteExpressionBacterialMutationS101T, D103E, S104T, D115E, N116Q, S117T, S122T, …PromoterT7Available sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 9acidA
Plasmid#231867PurposeBacterial expression of N-terminally 6His tagged Gcn4 9acidADepositorInsertGcn4 9acidA
UseTags6xHis, TEV cleavage siteExpressionBacterialMutationE109A, E111A, E114A, D115A, E119A, D125A, D127A, …PromoterT7Available sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80EN5-Elen0515-eCadh-noAccs(ElA2)
Plasmid#225313PurposeExpresses Eggerthella lenta A2 eCadh without accessory genes (inactive) in Gordonibacter urolithinfaciens DSM 27213DepositorInserteCadh (Elen_0515 )
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80ENSF7.1-Dadh-ΔElen0469(ElA2)
Plasmid#225315PurposeExpresses Eggerthella lenta A2 Dadh (ΔElen_0469) in Gordonibacter urolithinfaciens DSM 27213DepositorInsertDadh (ΔElen_0469)
UseTagsHis8ExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80ENSF7.4-Dadh-ΔElen0474(ElA2)
Plasmid#225319PurposeExpresses Eggerthella lenta A2 Dadh (ΔElen_0474) in Gordonibacter urolithinfaciens DSM 27213DepositorInsertDadh (ΔElen_0474)
UseTagsHis8ExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80ENSF7.7-Dadh-ΔElen0477(ElA2)
Plasmid#225322PurposeExpresses Eggerthella lenta A2 Dadh (ΔElen_0477) in Gordonibacter urolithinfaciens DSM 27213DepositorInsertDadh (ΔElen_0477)
UseTagsHis8ExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80ENSF7.8-Dadh-ΔElen0478(ElA2)
Plasmid#225323PurposeExpresses Eggerthella lenta A2 Dadh (ΔElen_0478) in Gordonibacter urolithinfaciens DSM 27213DepositorInsertDadh (ΔElen_0478)
UseTagsHis8ExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80ENSF7.9-Dadh-ΔElen0481(ElA2)
Plasmid#225324PurposeExpresses Eggerthella lenta A2 Dadh (ΔElen_0481) in Gordonibacter urolithinfaciens DSM 27213DepositorInsertDadh (ΔElen_0481)
UseTagsHis8ExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80ENSF7.10-Dadh-ΔElen0482(ElA2)
Plasmid#225325PurposeExpresses Eggerthella lenta A2 Dadh (ΔElen_0482) in Gordonibacter urolithinfaciens DSM 27213DepositorInsertDadh (ΔElen_0482)
UseTagsHis8ExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD70DA8-DadhA(cHis8)BC-DadRS-noAccs(ElA2)
Plasmid#225326PurposeExpresses Eggerthella lenta A2 Dadh in Eggerthella lenta DSM 2243DepositorInsertDadh
UseTagsHis8ExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80ENSF6-Elen0497-ElVadh-Accs(ElDSM2243)
Plasmid#225301PurposeExpresses Eggerthella lenta DSM 2243 El Vadh in Gordonibacter urolithinfaciens DSM 27213DepositorInsertEl Vadh
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80ENSF7-DadhA(cHis8)BC-DadRS-Accs(ElA2)
Plasmid#225302PurposeExpresses Eggerthella lenta A2 Dadh in Gordonibacter urolithinfaciens DSM 27213DepositorInsertDadh
UseTagsHis8ExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80ENSF8-HcdhA(cHis8)BC-HcdR-Accs(ElA2)
Plasmid#225303PurposeExpresses Eggerthella lenta A2 Hcdh in Gordonibacter urolithinfaciens DSM 27213DepositorInsertHcdh
UseTagsHis8ExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80ENSF18-C1878_02750_GsVadh(Gs28C)
Plasmid#225304PurposeExpresses Gordonibacter sp. 28C Gs Vadh in Gordonibacter urolithinfaciens DSM 27213DepositorInsertGs Vadh
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80EN3-Elen0507-Cadh-noAccs(ElA2)
Plasmid#225311PurposeExpresses Eggerthella lenta A2 Cadh without accessory genes (inactive) in Gordonibacter urolithinfaciens DSM 27213DepositorInsertCadh
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only