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Plasmid#154159PurposeFirefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, with 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.DepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutation4 wt NF-κB sites were replaced with Rel1A-specifi…PromoterAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLJM60-S6K1(T389S) barcoded
Plasmid#48800Purposeexpresses mutant S6K1DepositorInsertS6K1 T389S (Rps6kb1 Rat)
UseLentiviralTagsFLAG and barcode: GGTACCExpressionMammalianMutationT389SPromoterCMVAvailable sinceDec. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAA3
Plasmid#154163PurposeFirefly luciferase under the control of 5 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.DepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutation20 wt NF-κB sites were replaced with Rel1A-specif…PromoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM5
Plasmid#154166PurposeAedes aegypti Rel2 (long isoform) expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.DepositorInsertRel2 (long isoform)
UseTagsFLAGExpressionInsectMutationPromoterIE1 promoter and hr5 enhancer from Autographa cal…Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM28
Plasmid#154165PurposeAedes aegypti Rel1A expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virusDepositorInsertRel1A
UseTagsFLAGExpressionInsectMutationPromoterIE1 promoter and hr5 enhancer from Autographa cal…Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAA2
Plasmid#154162PurposeFirefly luciferase under the control of 4 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.DepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutation16 wt NF-κB sites were replaced with Rel1A-specif…PromoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAA1
Plasmid#154161PurposeFirefly luciferase under the control of 3 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.DepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutation12 wt NF-κB sites were replaced with Rel1A-specif…PromoterAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM62
Plasmid#154160PurposeFirefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, with 4 Rel2-specific (VII) NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.DepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutation4 wt NF-κB sites were replaced with Rel2-specific…PromoterAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM59
Plasmid#154158PurposeFirefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, a TATA box and Kozak sequence of the Metchnikowin gene and additional NF-κB site.DepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutationPromoterEnhancer for the Drosophila melanogaster Metchnik…Available sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM52
Plasmid#154155PurposeFirefly luciferase under the control of the Drosophila melanogaster minimal Hsp70 promoter with 8 copies of the Aedes aegypti Rel2 NF-κB siteDepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutationPromoterDrosophila melanogaster minimal Hsp70 promoter wi…Available sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM51
Plasmid#154154PurposeFirefly luciferase under the control of the Drosophila melanogaster minimal Hsp70 promoter with 8 copies of the Aedes aegypti Rel1A NF-κB siteDepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutationPromoterDrosophila melanogaster minimal Hsp70 promoter wi…Available sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCWori cytochrome P450-BM3h 9D7
Plasmid#61308PurposeExpresses Cytochrome P450-BM3h 9D7 with C-terminal 6-His tagDepositorInsertCytochrome P450-BM3 heme domain 9D7
UseTags6xHisExpressionBacterialMutationT268A, I263A, T438V, A328GPromoterTacAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-SPOTlight-U2GCR
Plasmid#164819PurposeReporter to measure ISR state-dependent protein synthesisDepositorInsertSPOTlight-U2GCR
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-MTOR-L1460P
Plasmid#69006Purposeactivating MTOR mutationDepositorInsertMTOR-L1460P (MTOR Human)
UseTagsFLAGExpressionMammalianMutationchange Leucine 1460 to ProlinePromoterAvailable sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-MTOR-C1483F
Plasmid#69008Purposeactivating MTOR mutationDepositorInsertMTOR-C1483F (MTOR Human)
UseTagsFLAGExpressionMammalianMutationchange Cysteine 1483 to PhenylalaninePromoterAvailable sinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBABE-HA-GOLPH3
Plasmid#21687DepositorInsertGolgi Phosphoprotein 3 (GOLPH3 Human)
UseRetroviralTagsHAExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpCas9-P2A-EGFP (RTW3027)
Plasmid#139987PurposeCMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationPromoterCMV and T7Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DIO-EF1α-mKate2-PDE4D3-Cat
Plasmid#169128PurposeAAV plasmid containing a mKate2 and a truncated PDE4D3DepositorInsertmKate2-PDE4DCatL
UseAAV and Cre/LoxTagsmKate2ExpressionMammalianMutationtruncated, Catalatic domain onlyPromoterEF1aAvailable sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-GRAB_rDA-mut
Plasmid#140558PurposeExpresses the red fluorescent DA-insensitive control sensor GRAB_rDA-mut in neuronsDepositorHas ServiceAAV9InsertRed fluorescent DA-insensitive sensor GRAB_rDA-mut (control sensor)
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DIO-EF1α-mKate2-biPAC
Plasmid#169127PurposeAAV plasmid containing a mKate2 and a light-gated adenylate cyclase biPACDepositorInsertmKate2-biPAC
UseAAV and Cre/LoxTagsmKate2ExpressionMammalianMutationcodon optimized to mousePromoterEF1aAvailable sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only