We narrowed to 5,091 results for: codon optimized

1. pLenti-HF1RA-PGK-Puro

   Plasmid

   #110860

   Purpose

   Lentiviral vector for constitutive expression of Cas9-HF1RA in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-HF1RA
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   R661A, Q695A, Q926A, and NLS sequence at the N-te…

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-HF1RA-P2A-Puro

   Plasmid

   #110853

   Purpose

   Lentiviral vector for constitutive expression of Cas9-HF1RA in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-HF1RA
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   R661A, Q695A, Q926A, and NLS sequence at the N-te…

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLenti-VQRRA-P2A-Puro

   Plasmid

   #110851

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VQRRA in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VQRRA
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   D1135V, R1335Q, T1337R and NLS sequence at the N-…

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLenti-VQRRA-P2A-GFP-PGK-Puro

   Plasmid

   #110864

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VQRRA-P2A-GFP in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VQRRA
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   D1135V, R1335Q, T1337R and NLS sequence at the N-…

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLenti-VQRRA-PGK-Puro

   Plasmid

   #110858

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VQRRA in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VQRRA
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   D1135V, R1335Q, T1337R and NLS sequence at the N-…

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDestTol2 ZfUbi::cMyc-oDam_f_GFPoNLS

   Plasmid

   #85821

   Purpose

   iDamID plasmid. To stably and ubiquitously express the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

   Depositor

   Joachim Wittbrodt

   Article

   Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

   Insert

   Zf Ubi::cMyc oDam_f_GFPoNLS
   

   Use

   Tol2 based vector

   Tags

   cMyc N terminal to oDam_f_GFPoNLS and oNLS (optim…

   Expression

   Mutation

   L122A. Codon optimization compatible to work in M…

   Promoter

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-VRERRA-PGK-Puro

   Plasmid

   #110859

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VRERRA in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VRERRA
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   D1135V, G1218R, R1335E, T1337R, and NLS sequence …

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLenti-VRERRA-P2A-GFP-PGK-Puro

   Plasmid

   #110865

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VRERRA-P2A-GFP in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VRERRA
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   D1135V, G1218R, R1335E, T1337R, and NLS sequence …

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLenti-VRERRA-P2A-Puro

   Plasmid

   #110852

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VRERRA in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VRERRA
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   D1135V, G1218R, R1335E, T1337R, and NLS sequence …

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLenti-VRER-P2A-GFP-PGK-Puro

    Plasmid

    #110862

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VRER-P2A-GFP (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VRER
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    D1135V, G1218R, R1335E, T1337R

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLenti-HF1-P2A-GFP-PGK-Puro

    Plasmid

    #110863

    Purpose

    Lentiviral vector for constitutive expression of Cas9-HF1-P2A-GFP (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-HF1
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    N497A, R661A, Q695A, Q926A

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV-T7-ABEmax-SpRY

    Plasmid

    #195279

    Purpose

    Allows for in vitro transcription of original pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025) without EGFP

    Depositor

    Carsten Lederer, Petros Patsali

    Insert

    human codon optimized ABEmax(7.10) SpCas9 variant named SpRY without EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHH0103 IQGAP1 WW domain

    Plasmid

    #104279

    Purpose

    Bacterial expression of WW domain from IQGAP1

    Depositor

    Marius Sudol, Sachdev Sidhu

    Insert

    IQGAP1 WW domain (IQGAP1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CBh-mKate2-IRES-MCS (WT-WT)

    Plasmid

    #105921

    Purpose

    AAV-mediated gene expression from CBh promoter. Contains canonical left and right AAV2 ITRs

    Depositor

    Marcella Patrick

    Has Service

    AAV2

    Inserts

    mKate2
    IRES-Multiple cloning site
    

    Use

    AAV

    Tags

    Expression

    Mutation

    codon optimized

    Promoter

    CBh and same CBh promoter as mKate2

    Available since

    Feb. 15, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pFLPo

    Plasmid

    #13792

    Purpose

    Expression of mouse codon-optimized FLP recombinase

    Depositor

    Philippe Soriano

    Article

    Raymond et al PLoS One. 2007 Jan 17;2(1):e162. doi: 10

    Insert

    FLPo
    

    Use

    Cloning vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. yo-msGFP2

    Plasmid

    #160471

    Purpose

    Encodes a yeast codon optimized version of msGFP2

    Depositor

    Benjamin Glick

    Article

    Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

    Insert

    yo-msGFP2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLV-HuGDNF

    Plasmid

    #182946

    Purpose

    To expres Human GDNF with optimized codon usage for better expression

    Depositor

    Pantelis Tsoulfas

    Insert

    Human GDNF (GDNF Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV enhancer

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. lvl0 LhGR

    Plasmid

    #153408

    Purpose

    Dex inducible system trans activator

    Depositor

    Naomi Nakayama

    Article

    Andreou et al bioRxiv, March 31, 2021

    Insert

    LhGR CDS
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Codon optimized

    Promoter

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti spCas9 T2A iRFP670 P2A puro

    Plasmid

    #122182

    Purpose

    The plasmid codes for a Flag-spCas9 protein, a iRFP670 fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.

    Depositor

    Raphael Gaudin

    Inserts

    spCas9 (NEWENTRY )
    iRFP670
    

    Use

    Lentiviral

    Tags

    T2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti spCas9 T2A mNeonGreen P2A puro

    Plasmid

    #122183

    Purpose

    The plasmid codes for a Flag-spCas9 protein, a mNeonGreen fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.

    Depositor

    Raphael Gaudin

    Inserts

    spCas9 (NEWENTRY )
    mNeonGreen
    

    Use

    Lentiviral

    Tags

    T2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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