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Plasmid#204292PurposeFor inserting a phiC31 attP site. HDR integrant is marked with removable 3XP3-DsRed cassette, which is expressed in eyes. DsRed difficult to see in wild type eyes if using LeicaM165 FC microscope.DepositorInsert3XP3-DsRed
UseCRISPRTagsExpressionMutationPromoter3XP3Available sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKM808
Plasmid#134181PurposeLentiviral sgRNA plasmid with blasticidin resistanceDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SCP1-dSa VPR mini.-2X snRP-1 Afp
Plasmid#99696PurposeExpresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Afp, vector allows for strong activation of mouse Afp, can be packaged and delivered as AAVDepositorInsertdCas9 and gRNA targeting Afp (Afp Synthetic)
UseAAVTagsVPR miniExpressionMutationdead Cas9PromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJAT58
Plasmid#204304PurposeExpresses jGCaMP7s-T2A-tdTomato under UAS control. HDR event marked with removable 3XP3-DsRed cassette expressed in eyes.DepositorInsert3XP3-DsRed
UseCRISPRTagsExpressionMutationPromoter3XP3Available sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJAT71
Plasmid#204305PurposeExpresses jGCaMP7s-T2A-tdTomato under UAS control. HDR event marked with removable ie1XP3-DsRed cassette expressed in abdomen and mouthparts.DepositorInsertie1-DsRed
UseCRISPRTagsExpressionMutationPromoterie1Available sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQCi-mB2m-sgRNA
Plasmid#154090PurposepQCi backbone with sgRNA targeting murine Beta-2-microglobulinDepositorInsertsgRNA targeting murine B2M locus
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDA52_Lenti.puro.U6_modified DR
Plasmid#196723PurposeFor cloning pairs of AsCas12a guides with two DRs that are as different as possible.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNPAS1-donor
Plasmid#176346PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertNPAS1 (NPAS1 Human)
UseCRISPRTagsGFPExpressionMutationPromoterAvailable sinceNov. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSOX15-donor
Plasmid#176356PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertSOX15 (SOX15 Human)
UseCRISPRTagsGFPExpressionMutationPromoterAvailable sinceNov. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPRDM15-donor
Plasmid#176373PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertPRDM15 (PRDM15 Human)
UseCRISPRTagsGFPExpressionMutationPromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZIC3-donor
Plasmid#176361PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertZIC3 (ZIC3 Human)
UseCRISPRTagsGFPExpressionMutationPromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTHAP12-donor
Plasmid#176348PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertTHAP12 (THAP12 Human)
UseCRISPRTagsGFPExpressionMutationPromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPOU6F1-donor
Plasmid#176359PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertPOU6F1 (POU6F1 Human)
UseCRISPRTagsGFPExpressionMutationPromoterAvailable sinceNov. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZNF608-donor
Plasmid#176362PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertZNF608 (ZNF608 Human)
UseCRISPRTagsGFPExpressionMutationPromoterAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZBTB4-donor
Plasmid#176374PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertZBTB4 (ZBTB4 Human)
UseCRISPRTagsGFPExpressionMutationPromoterAvailable sinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZNF624-donor
Plasmid#132511PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertZNF624 (ZNF624 Human)
UseCRISPRTagsGFPExpressionMutationnonePromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZNF8-donor
Plasmid#136689PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertZNF8 (ZNF8 Human)
UseCRISPRTagsGFPExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTOX.1.0-gDNA
Plasmid#132470PurposeCRISPR/Cas9 plasmid to create GFP fusion proteinsDepositorInsertTOX (TOX Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZNF132.1.0-gDNA
Plasmid#132468PurposeCRISPR/Cas9 plasmid to create GFP fusion proteinsDepositorInsertZNF132 (ZNF132 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSOX13.1.0-gDNA
Plasmid#132466PurposeCRISPR/Cas9 plasmid to create GFP fusion proteinsDepositorInsertSOX13 (SOX13 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only