We narrowed to 308 results for: G2

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pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair2

Plasmid

#98973

Purpose

Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 96bp 5' overhangs. TagBFP.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA sgRNAs pair 2 and Cas9(D10A)

Expression

Mammalian

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair2

Plasmid

#98973

Purpose

Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 96bp 5' overhangs. TagBFP.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA sgRNAs pair 2 and Cas9(D10A)

Expression

Mammalian

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair3

Plasmid

#98974

Purpose

Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 37bp 3' overhangs. TagBFP.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA sgRNAs pair 3 and Cas9(D10A)

Expression

Mammalian

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair3

Plasmid

#98974

Purpose

Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 37bp 3' overhangs. TagBFP.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA sgRNAs pair 3 and Cas9(D10A)

Expression

Mammalian

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGMC00020 (aka pRC0431)

Plasmid

#195304

Purpose

Lentiviral expression vector derived from LEGO-G2 with blasticidin selection

Depositor

Raj Chari

Type

Empty backbone

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGMC00020 (aka pRC0431)

Plasmid

#195304

Purpose

Lentiviral expression vector derived from LEGO-G2 with blasticidin selection

Depositor

Raj Chari

Type

Empty backbone

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGMC00021 (aka pRC0432)

Plasmid

#195305

Purpose

Lentiviral expression vector derived from LEGO-G2 with puromycin selection

Depositor

Raj Chari

Type

Empty backbone

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGMC00021 (aka pRC0432)

Plasmid

#195305

Purpose

Lentiviral expression vector derived from LEGO-G2 with puromycin selection

Depositor

Raj Chari

Type

Empty backbone

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
RepairTemplate_AAVS1-OsTIR1-F74G-SNAP

Plasmid

#217665

Purpose

Repair template to integrate OsTIR1-F74G-SNAP into the human AAVS1 safe harbour locus

Depositor

Daniel Gerlich

Article

Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

Insert

OsTir1 F74G-SNAP-Blast-AAVS1

Tags

SNAP

Expression

Bacterial and Mammalian

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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RepairTemplate_AAVS1-OsTIR1-F74G-SNAP

Plasmid

#217665

Purpose

Repair template to integrate OsTIR1-F74G-SNAP into the human AAVS1 safe harbour locus

Depositor

Daniel Gerlich

Article

Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

Insert

OsTir1 F74G-SNAP-Blast-AAVS1

Tags

SNAP

Expression

Bacterial and Mammalian

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
sgRNA1_Cas9-hGem_Smc4

Plasmid

#217667

Purpose

expresses Cas9-hGem and guideRNA for tagging of SMC4

Depositor

Daniel Gerlich

Article

Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

Insert

sgRNA1 targeting SMC4 C-terminal (SMC4 Human)

Use

CRISPR

Expression

Bacterial and Mammalian

Promoter

U6

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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sgRNA1_Cas9-hGem_Smc4

Plasmid

#217667

Purpose

expresses Cas9-hGem and guideRNA for tagging of SMC4

Depositor

Daniel Gerlich

Article

Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

Insert

sgRNA1 targeting SMC4 C-terminal (SMC4 Human)

Use

CRISPR

Expression

Bacterial and Mammalian

Promoter

U6

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC CBA-SpCas9N863Anickase.EF1a-BFP.sgLMNApair1

Plasmid

#98976

Purpose

Cas9 Homologous Recombination Reporter. SpCas9N863A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 44bp 3' overhangs. TagBFP.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA sgRNAs pair 1 and Cas9(N863A)

Expression

Mammalian

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC CBA-SpCas9N863Anickase.EF1a-BFP.sgLMNApair1

Plasmid

#98976

Purpose

Cas9 Homologous Recombination Reporter. SpCas9N863A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 44bp 3' overhangs. TagBFP.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA sgRNAs pair 1 and Cas9(N863A)

Expression

Mammalian

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair1

Plasmid

#98972

Purpose

Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNA targeting hLMNA. Generates breaks with 44bp 5' overhangs. TagBFP.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA sgRNAs pair 1 and Cas9(D10A)

Expression

Mammalian

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair1

Plasmid

#98972

Purpose

Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNA targeting hLMNA. Generates breaks with 44bp 5' overhangs. TagBFP.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA sgRNAs pair 1 and Cas9(D10A)

Expression

Mammalian

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
TFORF2151

Plasmid

#142920

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

FOXG1 (FOXG1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TFORF2151

Plasmid

#142920

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

FOXG1 (FOXG1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEG302 22aa SunTag NtDRMcd SUP

Plasmid

#115489

Purpose

CRISPR-Cas9 SunTag system to target NtDRMcd to the SUPERMAN locus with two guide RNAs

Depositor

Steven Jacobsen

Article

Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

Insert

g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pEG302 22aa SunTag NtDRMcd SUP

Plasmid

#115489

Purpose

CRISPR-Cas9 SunTag system to target NtDRMcd to the SUPERMAN locus with two guide RNAs

Depositor

Steven Jacobsen

Article

Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

Insert

g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge