We narrowed to 12,131 results for: LEA
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Plasmid#102831PurposeBacterial expression of SpyTag002 fused to maltose binding proteinDepositorInsertSpyTag002-MBP
TagsHis6 and Thrombin cleavage siteExpressionBacterialMutationPeptide tag added to the N-terminus of MBP via a …PromoterT7Available SinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1 Lysosomal-METRIQ
Plasmid#135401PurposeExpresses lysosomal-METRIQ (DNAse II-sfGFP together with cytosolic RFP) to measure lysosomal activityDepositorInsertDNAse II alpha (DNASE2 Human)
UseLentiviralTagsT2A, mCherry, and sfGFPExpressionMammalianMutationSingle-nucleotide polymorphism C432TAvailable SinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDH-NLS-copGFP-EF1-BlastiS
Plasmid#132772PurposeSpills into the cytoplasm during nuclear envelope rupture.DepositorInsertNLS-copGFP
UseLentiviralExpressionMammalianAvailable SinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
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7TFP CDH1 reporter
Plasmid#91704Purposelentiviral luciferase reporter containing CDH1 promoterDepositorInsertCDH1 promoter (CDH1 Human)
UseLentiviral and LuciferaseTagsluciferaseExpressionMammalianPromoterCDH1Available SinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
GW1-Peredox-mCherry-NLS
Plasmid#32381DepositorInsertPeredox-mCherry-NLS
TagsNuclear Localization SeqExpressionMammalianPromoterCMVAvailable SinceOct. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pPEP-TEV cNup98 (1-498)
Plasmid#38037DepositorInsertNucleoporin 98 (NUP98 Human)
TagsHis-tag, 3CysteineExpressionBacterialMutationamino acids 1-498PromoterT7Available SinceSept. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
AviTag-SpyCatcher
Plasmid#72326PurposeBacterial expression of SpyCatcher with a peptide tag enabling site-specific biotinylation.DepositorInsertAviTag-SpyCatcher
TagsN-term His6 tag and TEV cleavage siteExpressionBacterialAvailable SinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
PEA1-GFP
Plasmid#171993PurposeDelivers all prime editing nickase components in a single, GFP selectable plasmidDepositorInsertCbH-Cas9(H840A)-RT-T2A-GFP, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
ExpressionMammalianPromoterCMV for Cas9, U6 for gRNAsAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET His6 Mocr TEV LIC cloning vector (1O)
Plasmid#29658DepositorTypeEmpty backboneTags6xHis, Mocr, and TEV cleavage siteExpressionBacterialPromoterT7-lacO (lactose/IPTG inducible)Available SinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-miR29b
Plasmid#21121DepositorInsertmiR-29b-1 (MIR29B1 Human)
ExpressionMammalianAvailable SinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAd/CMV/DAX1-IRES-nEGFP
Plasmid#29752DepositorInsertdosage-sensitive sex reversal, adrenal hypoplasia critical region, on chromosome X, gene 1 (NR0B1 Human)
TagsEGFP, IRES, and Nuclear localization sequenceExpressionMammalianMutationNo mutations; Reporter is nuclear localized EGFP;…Available SinceOct. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLenti-DsRed_IRES_SNCA:EGFP
Plasmid#92195PurposeLentiviral overexpression vector to make stable bicistronic cell line for α-Syn screenDepositorInsertDsRed-IRES-SNCA-EGFP
UseLentiviralExpressionMammalianAvailable SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBAD-LacI
Plasmid#46394PurposeOverproduction of LacIDepositorInsertLacI
Tags6xHIS tagExpressionBacterialMutationsilent T to C mutation at position 19Promoterarabinose promoterAvailable SinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
TALEN-mROSA26 ELD
Plasmid#60026PurposeExpression of a TALEN protein specific for mouse ROSA26 locus.DepositorInsertTALEN-ROSA26
UseTALENExpressionMammalianAvailable SinceNov. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMSCV PIG IMP-1-short
Plasmid#21659DepositorInsertIGF-II mRNA-binding protein 1 (IGF2BP1 Human)
UseRetroviralTagsGFP and IRESExpressionMammalianAvailable SinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEJS613_pTetR-P2A-BFPnls/sgTelo
Plasmid#108649PurposeTelomere-targeting Spy sgRNA under U6 promoterDepositorInsertsTelomere-targeting sgRNA
TetR-P2A-BFP
UseCRISPR and LentiviralTagsNoExpressionMammalianPromoterU6 and hPGKAvailable SinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX330A-1x3
Plasmid#58767PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianPromoterCBhAvailable SinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJ404-DogCatcher-sfGFP
Plasmid#171930PurposeExpress DogCatcher-superfolder GFP protein in bacterial cytoplasmDepositorInsertDogCatcher-sfGFP
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT5Available SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3s-Cas9HC
Plasmid#43945DepositorInsertCas9
UseCRISPRTagsHA epitope and NLSExpressionMammalianPromoterCMVAvailable SinceApril 24, 2013AvailabilityAcademic Institutions and Nonprofits only