We narrowed to 12,194 results for: LEA
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Plasmid#82034PurposePlasmid for constitutive or doxycycline-inducible expression of H129N nuclease-dead mutant of human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.DepositorInsertMRE11 (MRE11 Human)
TagsHAExpressionMammalianMutationNuclease-dead mutant: H129N MRE11PromoterCMV-tetAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCS2 Notch1 ΔEMV-6MT
Plasmid#41737DepositorInsertNotch-1 Extracellular-domain-deleted construct (Notch1 Mouse)
Tags6xMycExpressionMammalianMutationContains murine Notch-1 protein fragment lacking …Available sinceJan. 28, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAcGP67A-TfR
Plasmid#12130DepositorPublicationInserttransferrin receptor (TFRC Human)
Tags6X-His tag, Factor Xa cleavage site, and leader p…ExpressionInsectMutationThe portion of the human TfR gene encoding residu…Available sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1 Lysosomal-METRIQ
Plasmid#135401PurposeExpresses lysosomal-METRIQ (DNAse II-sfGFP together with cytosolic RFP) to measure lysosomal activityDepositorInsertDNAse II alpha (DNASE2 Human)
UseLentiviralTagsT2A, mCherry, and sfGFPExpressionMammalianMutationSingle-nucleotide polymorphism C432TAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDH-NLS-copGFP-EF1-BlastiS
Plasmid#132772PurposeSpills into the cytoplasm during nuclear envelope rupture.DepositorInsertNLS-copGFP
UseLentiviralExpressionMammalianAvailable sinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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GW1-Peredox-mCherry-NLS
Plasmid#32381DepositorPublicationInsertPeredox-mCherry-NLS
TagsNuclear Localization SeqExpressionMammalianPromoterCMVAvailable sinceOct. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pPEP-TEV cNup98 (1-498)
Plasmid#38037DepositorInsertNucleoporin 98 (NUP98 Human)
TagsHis-tag, 3CysteineExpressionBacterialMutationamino acids 1-498PromoterT7Available sinceSept. 14, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AviTag-SpyCatcher
Plasmid#72326PurposeBacterial expression of SpyCatcher with a peptide tag enabling site-specific biotinylation.DepositorInsertAviTag-SpyCatcher
TagsN-term His6 tag and TEV cleavage siteExpressionBacterialAvailable sinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJ404-DogCatcher-sfGFP
Plasmid#171930PurposeExpress DogCatcher-superfolder GFP protein in bacterial cytoplasmDepositorInsertDogCatcher-sfGFP
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT5Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PEA1-GFP
Plasmid#171993PurposeDelivers all prime editing nickase components in a single, GFP selectable plasmidDepositorInsertCbH-Cas9(H840A)-RT-T2A-GFP, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
ExpressionMammalianPromoterCMV for Cas9, U6 for gRNAsAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET His6 Mocr TEV LIC cloning vector (1O)
Plasmid#29658DepositorTypeEmpty backboneTags6xHis, Mocr, and TEV cleavage siteExpressionBacterialPromoterT7-lacO (lactose/IPTG inducible)Available sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-miR29b
Plasmid#21121DepositorInsertmiR-29b-1 (MIR29B1 Human)
ExpressionMammalianAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAd/CMV/DAX1-IRES-nEGFP
Plasmid#29752DepositorInsertdosage-sensitive sex reversal, adrenal hypoplasia critical region, on chromosome X, gene 1 (NR0B1 Human)
TagsEGFP, IRES, and Nuclear localization sequenceExpressionMammalianMutationNo mutations; Reporter is nuclear localized EGFP;…Available sinceOct. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMAL-7xHis-TEV-hexa
Plasmid#222868PurposeExpresses His-tagged mutant (C19V/L56V/C110V/C130S/S135G/S219V) TEV protease in E. coliDepositorInsertTEV protease
Tags7xHis and MBPExpressionBacterialMutationC19V, L56V, C110V, C130S, S135G, S219V. Insert in…Available sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn Con/Fon EYFP (AAV Retrograde)
Viral prep#55650-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-hSyn Con/Fon EYFP (#55650). In addition to the viral particles, you will also receive purified pAAV-hSyn Con/Fon EYFP plasmid DNA. Synapsin driven, Cre and Flp-dependent EYFP expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEYFP (Cre- and Flp-dependent)Available sinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-DsRed_IRES_SNCA:EGFP
Plasmid#92195PurposeLentiviral overexpression vector to make stable bicistronic cell line for α-Syn screenDepositorInsertDsRed-IRES-SNCA-EGFP
UseLentiviralExpressionMammalianAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGCaMP6m-N3-TPC2
Plasmid#80147PurposeGCaMP6 is a genetically encoded fluorescent Ca2+ sensor . TPC2-GCaMP6 allows the visualization of Ca2+ release from intracellular Ca2+ stores in real timeDepositorAvailable sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBAD-LacI
Plasmid#46394PurposeOverproduction of LacIDepositorInsertLacI
Tags6xHIS tagExpressionBacterialMutationsilent T to C mutation at position 19Promoterarabinose promoterAvailable sinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
TALEN-mROSA26 ELD
Plasmid#60026PurposeExpression of a TALEN protein specific for mouse ROSA26 locus.DepositorInsertTALEN-ROSA26
UseTALENExpressionMammalianAvailable sinceNov. 25, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by