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HJP-207-IVS-Syn21-gp41-1C-CreBC-WPREUw

Plasmid

#162479

Purpose

This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

Depositor

Benjamin White

Article

Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

Insert

CreBC

Use

Cre/Lox

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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HJP-177-IVS-Syn21-gp41-1C-CreBC-WPREw

Plasmid

#162474

Purpose

This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

Depositor

Benjamin White

Article

Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

Insert

CreBC

Use

Cre/Lox

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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HJP-473-DNE>FLP-T2A-CreAB-NrdJ-1N

Plasmid

#162480

Purpose

This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

Depositor

Benjamin White

Article

Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

Insert

DNE-Frterminator

Use

Cre/Lox

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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ADP1-ISx

Bacterial Strain

#216551

Purpose

Acinetobacter baylyi strain with transposons deleted from its genome

Depositor

Jeffrey Barrick

Article

Suarez et al Appl Environ Microbiol. 2017 Aug 17;83(1

Bacterial Resistance

None

Available Since

June 10, 2024

Availability

Academic Institutions and Nonprofits only
DHIP1

Bacterial Strain

#226851

Purpose

Expresses HIP1 methyltransferase to methylate plasmids for delivery to cyanobacteria

Depositor

David Nielsen

Article

Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:

Bacterial Resistance

Streptomycin

Species

Escherichia coli

Available Since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only
ATMW1

Bacterial Strain

#218768

Purpose

E. coli strain, genotype: EcNR1 pUltraG ScW40 CCA trpS::ZeoR trpT::gentR dgalK lambdaRED::galK

Depositor

Abhishek Chatterjee

Article

Italia et al Nat Chem Biol. 2017 Apr;13(4):446-450. d

Bacterial Resistance

Spectinomycin (50 ug/mL), Gentamycin (10 ug/mL)

Species

Escherichia coli

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only
ATMW-BL21

Bacterial Strain

#218769

Purpose

E. coli strain, genotype: pUltraG ScW40CCA BL21 trpT::gentR trpS::zeoR

Depositor

Abhishek Chatterjee

Article

Ficaretta et al J Mol Biol. 2022 Apr 30;434(8):167304. d

Bacterial Resistance

Spectinomycin (50 ug/mL), Gentamycin (10 ug/mL)

Species

Escherichia coli

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only
Anti-APP [6E10-A5] (Recombinant Antibody)

Antibody

#226376-rAb

Purpose

Anti-APP (Human) recombinant mouse monoclonal antibody

Depositor

Melina Fan

Article

Goepfert et al Immunity. 2020 Mar 17;52(3):499-512.e5.

Recommended Applications

Immunohistochemistry

Reactivity

Human and Mouse

Source Species

Mouse

Isotype

IgG2a

Trial Size

Available to purchase

Available Since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits
BL21(DE3)-R3-pRARE2

Bacterial Strain

#26242

Purpose

Phage-resistant derivative of BL21(DE3), which has been transformed with plasmid pRARE2, which carries seven rare-codon tRNA genes.

Depositor

Opher Gileadi

Article

Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

Bacterial Resistance

Chloramphenicol

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only
delta-ZYA

Bacterial Strain

#52695

Purpose

MG1655 with Lac Operon removed from native locus

Depositor

Tanja Kortemme

Article

Eames et al Science. 2012 May 18;336(6083):911-5. do

Bacterial Resistance

None

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only
V. natriegens NC7

Bacterial Strain

#215356

Purpose

Vibrio natriegens with genomically integrated natural competence regulator, TfoX. For zero-capital molecular biology applications.

Depositor

Buz Barstow

Article

Specht et al PNAS Nexus. 2024 Feb 13;3(2):pgad444. do

Bacterial Resistance

None

Available Since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only
V. natriegens NC1

Bacterial Strain

#215355

Purpose

Vibrio natriegens with genomically integrated natural competence regulator, TfoX, with chloramphenicol resistance. For zero-capital molecular biology applications.

Depositor

Buz Barstow

Article

Specht et al PNAS Nexus. 2024 Feb 13;3(2):pgad444. do

Bacterial Resistance

None

Available Since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only
CRISPi Kit

Plasmid Kit

#1000000136

Purpose

Plasmids for performing CRISPR/Cas9 mediated gene and genome editing in Pichia pastoris.

Depositor

Brigitte Gasser

Application

Genome Editing

Vector Type

Yeast Expression

Editing Type

CRISPR

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only
EcAR7

Bacterial Strain

#52055

Purpose

Strain for use in Rinehart Lab Phosphoprotein synthesis kit

Depositor

Jesse Rinehart

Article

Heinemann et al FEBS Lett. 2012 Oct 19;586(20):3716-22.

Bacterial Resistance

None

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
pUC57-mitoTPNOX

Plasmid

#87854

Purpose

This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.

Depositor

Vamsi Mootha

Article

Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/

Insert

mitoTPNOX

Tags

C-terminal FLAG tag with a linker and mitochondri…

Expression

Bacterial

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pMT-Hrp48-ADARcd-E488Q-V5

Plasmid

#139685

Purpose

Expresses the hyperactive form of the catalytic domain of the ADAR protein fused to HRP-48 for use in "TRIBE" assays

Depositor

Michael Rosbash

Article

Xu et al RNA. 2018 Feb;24(2):173-182. doi: 10.126

Insert

Hrp48, ADAR (Adar Fly)

Tags

V5

Expression

Bacterial and Insect

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pBITE-CD19-CD3

Plasmid

#190677

Purpose

Encodes a modularized form of HD37-OKT3 bispecific antibody

Depositor

Scott McComb

Article

Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

Insert

Modularized-HD37-modularized-OKT3-P2A-NeonGreen

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Promoter

EFS

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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scFLARE1

Plasmid

#158738

Purpose

Mammalian expression of scFLARE1 component

Depositor

Alice Ting

Article

Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

Insert

scFLARE1

Use

AAV

Tags

Flag, V5

Expression

Mammalian

Mutation

Truncated form of TEV protease (220-242 amino aci…

Promoter

CMV

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pET15b-RvLEAMshort

Plasmid

#218122

Purpose

Expresses truncated RvLEAM protein under IPTG induction in bacterial host cells.

Depositor

Ci Ji Lim

Article

Abe et al bioRxiv [Preprint]. 2024 Feb 11:2024.02.

Insert

RvLEAMshort

Tags

6xHIS

Expression

Bacterial

Mutation

Truncated form (58-181aa)

Promoter

T7

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 mycBioID

Plasmid

#35700

Purpose

To fuse your protein of interest to the C-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); myc tag

Depositor

Kyle Roux

Article

Roux et al J Cell Biol. 2012 Mar 12.

Has Service

Cloning Grade DNA

Insert

BirA

Tags

Myc

Expression

Mammalian

Mutation

R118G (highly promiscuous form)

Promoter

cmv

Available Since

April 5, 2012

Availability

Academic Institutions and Nonprofits only

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