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pLH-nmsgRNA1.1

Plasmid

#64115

Purpose

Vector for expression of Nm sgRNA1.1 in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Nm sgRNA1.1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pTetO-ISce1-TetO

Plasmid

#58506

Purpose

ISce1 restriction site is flanked by Tet operator repeats, 96 on each side

Depositor

Tom Misteli

Article

Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

Insert

Tet repeats flanking ISce1 restriction site

Expression

Bacterial

Promoter

no promoter

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pQCasTns(Ptr)-IS186

Plasmid

#175581

Purpose

Expression of CRISPR-associated transposases

Depositor

Sheng Yang

Article

Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

Insert

PtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, PtrTnsABC, CRISPR(IS186)

Expression

Bacterial

Mutation

NO

Available Since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTcI-Wasabi

Plasmid

#86101

Purpose

mWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TcI
mWasabi

Tags

6xHis

Expression

Bacterial

Mutation

A67T

Promoter

pR/pL and pR/pL, LacI

Available Since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pJUMP27ts-1A(sfGFP)

Plasmid

#126975

Purpose

Level 1 vector. sfGFP reporter. Thermosensitive origin pSC101 (low copy number)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

RepA A56V thermosensitive mutation

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pTlpA-Wasabi

Plasmid

#85455

Purpose

mWasabi reporter gene gated by wild type TlpA

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TlpA
mWasabi

Tags

6xHis

Expression

Bacterial

Mutation

D341A

Promoter

TlpA and TlpA, LacI

Available Since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pTns(Ptr)

Plasmid

#175576

Purpose

Expression of CRISPR-associated transposases

Depositor

Sheng Yang

Article

Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

Insert

PtrTnsABC

Expression

Bacterial

Mutation

NO

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLH-stsgRNA3.1

Plasmid

#64118

Purpose

Vector for expression of St1 sgRNA3.1 in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 sgRNA3.1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-nmdCas9-3XGFP

Plasmid

#64109

Purpose

Expression of Nm dCas9-3XGFP in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Nm dCas9

Use

CRISPR and Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Promoter

CMV-TO

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pGilesSHdI_psmyc_His6_triple red

Plasmid

#170047

Purpose

Mono-Responsive Reporter Gene Constructs for triple red

Depositor

Clifton Barry III

Article

Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

Inserts

mScarlet
COmScarlet
mRuby3

Use

Unspecified

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pUNIV backbone

Plasmid

#24705

Depositor

Cynthia Czajkowski

Article

Venkatachalan et al Pflugers Arch. 2007 Apr . 454(1):155-63.

Type

Empty backbone

Use

Xenopus oocyte expression

Expression

Bacterial, Mammalian, and Y…

Available Since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-dCas9-GFP

Plasmid

#64106

Purpose

Expression of Sp dCas9-GFP in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp dCas9

Use

CRISPR and Lentiviral

Tags

sfGFP

Expression

Mammalian

Promoter

CMV-TO

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-mTID-HA3-HIS3MX6

Plasmid

#227983

Purpose

To insert biotin ligase, miniTurboID, at the C-terminus of genes; yeast BioID,HIS3MX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

miniTurboID

Tags

HA tag

Expression

Yeast

Available Since

Dec. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pTol2-rtn1a-KALTA4

Plasmid

#239989

Purpose

Expresses GAL4 under control of the rtn1a promoter

Depositor

Summer Thyme

Article

Moyer et al bioRxiv [Preprint]. 2025 Mar 5:2025.02.2

Inserts

KALTA4
EGFP

Use

Zebrafish tol2/phic31 compatible

Promoter

myl7 and rtn1a

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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915-Plakophilin 2a-Flag

Plasmid

#32230

Depositor

Kathleen Green

Article

Chen et al J Biol Chem. 2002 Mar 22;277(12):10512-2

Insert

Plakophilin 2a (PKP2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 26, 2011

Availability

Academic Institutions and Nonprofits only
pDONR221-TFAM

Plasmid

#166217

Purpose

Gateway entry vector for N. furzeri TFAM

Depositor

Daniel Jarosz

Article

Chen et al Dev Cell. 2024 Jul 22;59(14):1892-1911.e

Insert

TFAM

Use

Gateway Cloning

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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JARID1CA

Plasmid

#74651

Purpose

Baculovirus expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

JARID1CA (KDM5C Human)

Use

Baculovirus

Tags

His-6-TEV

Promoter

PH

Available Since

April 21, 2016

Availability

Academic Institutions and Nonprofits only
pSKI074

Plasmid

#11571

Depositor

Detlef Weigel

Article

Weigel et al Plant Physiol. 2000 Apr . 122(4):1003-13

Insert

CaMV 35S enhancers

Use

Activation tagging

Expression

Plant

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

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pKIKOlacZCm

Plasmid

#46764

Purpose

E.coli genomic integration at lacZ

Depositor

Lars Nielsen, Claudia Vickers

Article

Sabri et al Microb Cell Fact. 2013 Jun 24;12(1):60.

Type

Empty backbone

Use

E.coli genomic integration

Available Since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pTcI42-Wasabi

Plasmid

#86118

Purpose

mWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI42

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TcI42
mWasabi

Tags

6xHis

Expression

Bacterial

Mutation

A67T, K6N, S33T, Y61H, L119P, F122C

Promoter

pR/pL and pR/pL, LacI

Available Since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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