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Addgene

We narrowed to 23,545 results for: CHI

Showing: 4151 - 4200 of 23545 results
  1. 3xFLAG-dCas9/p-bacteria

    Plasmid
    #64325
    Purpose
    Expresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Insert
    3xFLAG-dCas9
    Use
    CRISPR; Tetracycline-inducible
    Tags
    3xFLAG
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Promoter
    pLtetO-1
    Available since
    Aug. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
  2. pMAL-c5x_UnaG

    Plasmid
    #163125
    Purpose
    A plasmid with UnaG coding sequence cloned into it. UnaG gene codon optimized for E. coli and B. theta. Expresses UnaG with N-term maltose-binding protein (MBP)-tag and C-term His-tag.
    Depositor
    Insert
    UnaG
    Use
    Tags
    His and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
  3. p006-GFP-Logic-AND

    Plasmid
    #112237
    Purpose
    GFP from jellyfish Aquorea sp. under D61 AND gate-like ara+lac promoter with AraC repressor; lacI repressor; kanamycin resistant.
    Depositor
    Inserts
    GFP under control of doubly-repressible promoter
    GFP
    araC
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    AND-gate and pLacI
    Available since
    Aug. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
  4. pTJV1Sc3-rpoB

    Plasmid
    #166983
    Purpose
    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ EcRecT recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance
    Depositor
    Insert
    RecT (recT E. coli)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pVvanCC
    Available since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
  5. pBlueScript-humanAPOBEC1

    Plasmid
    #134606
    Purpose
    Plasmid bearing APOBEC1 coding sequence. In presence of its cofactor and overexpressing an artificial reporter with editable CAA codon in APOB sequence we can visualize its editing activity
    Insert
    humanAPOBEC1 (APOBEC1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Beta actin
    Available since
    Dec. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
  6. pGBW-m4046852

    Plasmid
    #145584
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 nsp8
    Insert
    SARS-CoV-2 nsp8 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 28, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 4151 - 4200 of 23545 results