We narrowed to 13,625 results for: try

1. pEF.myc.ER-E2-Crimson

   Plasmid

   #38770

   Purpose

   In vivo visualization of the endoplasmic reticulum (can be used for colocalization studies)

   Depositor

   Benjamin Glick

   Article

   Strack et al Biochemistry. 2009 Sep 8;48(35):8279-81.

   Insert

   E2-Crimson
   

   Use

   Tags

   ER retention signal (KDEL), ER signal sequence (M…

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28b-pchG WT

   Plasmid

   #87107

   Purpose

   expresses PchG WT, the thiazolinyl imine reductase from Pseudomonas aeruginosa

   Depositor

   Audrey Lamb

   Article

   Meneely et al Biochemistry. 2016 Sep 27;55(38):5423-33

   Insert

   pchG WT
   

   Use

   Tags

   his-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28b-irp3 WT

   Plasmid

   #87106

   Purpose

   expresses Irp3 WT, the thiazolinyl imine reductase from Yersinia enterocolitica

   Depositor

   Audrey Lamb

   Article

   Meneely et al Biochemistry. 2012 Nov 6;51(44):9002-13.

   Insert

   irp3 WT
   

   Use

   Tags

   his-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pABY-c45

   Plasmid

   #201771

   Purpose

   Expresses mNeonGreen-tagged NbALFA under TEF1 promoter in yeast

   Depositor

   Saravanan Palani

   Article

   Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

   Insert

   NbALFA
   

   Use

   Tags

   40aa Linker, mNeonGreen

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR221-SNRPA1

   Plasmid

   #166213

   Purpose

   Gateway entry vector for N. furzeri SNRPA1

   Depositor

   Daniel Jarosz

   Article

   Chen et al Dev Cell. 2024 Jul 22;59(14):1892-1911.e

   Insert

   SNRPA1
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBad-CA

   Plasmid

   #105665

   Purpose

   expresses thermostable variant of human carbonic anhydrase II with C-terminal 6x his tag

   Depositor

   Ryan Mehl

   Article

   Mehl et al 2020

   Insert

   human carbonic anhydrase II thermostable variant (CA2 Human)
   

   Use

   Tags

   6x his tag

   Expression

   Bacterial

   Mutation

   Promoter

   pBad/arabinose

   Available since

   March 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. FLAG-3C-sGFP11_pcDNA3.1(-)

   Plasmid

   #182932

   Purpose

   For expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP11 fragment

   Depositor

   William Weis

   Article

   Bruguera et al J Biol Chem. 2022 Jan 21:101628. doi: 10

   Type

   Empty backbone

   Use

   Tags

   3C-cleavable split GFP 11, FLAG tag, and HA signa…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. FLAG-3C-sGFP1-10_pcDNA3.1(-)

   Plasmid

   #182931

   Purpose

   For expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP1-10 fragment

   Depositor

   William Weis

   Article

   Bruguera et al J Biol Chem. 2022 Jan 21:101628. doi: 10

   Type

   Empty backbone

   Use

   Tags

   3C-cleavable split GFP 1-10, FLAG tag, and HA sig…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA-NHERF1

   Plasmid

   #31650

   Depositor

   Edward Weinman

   Article

   Weinman et al Biochemistry. 2003 Nov 4. 42(43):12662-8

   Insert

   solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (SLC9A3R1 Rabbit)
   

   Use

   Tags

   His & S tag

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   Feb. 14, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMSCVpuro-TagRFP657

    Plasmid

    #96939

    Purpose

    Retroviral plasmid containing TagRFP657 for labeling of cells

    Depositor

    Lee Grimes

    Article

    Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

    Insert

    Tag Red Fluorescent Protein 657
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. RGG-BcLOV-mCh

    Plasmid

    #208276

    Purpose

    Expresses RGG-BcLOV fusion for optogenetic membrane recruitment. Includes an mCherry tag.

    Depositor

    Lukasz Bugaj

    Article

    Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

    Insert

    RGG-BcLOV-mCh
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. Fus-BcLOV-mCh

    Plasmid

    #208277

    Purpose

    Expresses Fus-BcLOV fusion for optogenetic membrane recruitment. Includes an mCherry tag.

    Depositor

    Lukasz Bugaj

    Article

    Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

    Insert

    Fus-BcLOV-mCh
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENTR4-GFP-C1 (w392-1)

    Plasmid

    #17396

    Purpose

    Gateway entry vector for N-term eGFP fusion, frame C1

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    GFP
    

    Use

    Entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAG244 iFAST

    Plasmid

    #130809

    Purpose

    Expresses iFAST in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Tebo et al Biochemistry. 2018 Oct 2;57(39):5648-565

    Insert

    iFAST
    

    Use

    Tags

    His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pME phluorin2-gpi

    Plasmid

    #73793

    Purpose

    ratiometric pHluorin2 with a gpi linkage sequence in a middle entry clone

    Depositor

    David Raible

    Article

    Stawicki et al Dis Model Mech. 2014 Jul;7(7):847-56. do

    Insert

    ratiometric pHluorin2-gpi
    

    Use

    Middle entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. MXS_CreERT2

    Plasmid

    #62427

    Purpose

    MXS_chaining vector with CreERT2

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    Tamoxifen inducible Cre recombinase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBad-mKalama1

    Plasmid

    #14892

    Purpose

    Bacterial expression of mKalama1 blue fluorescent protein

    Depositor

    Robert Campbell

    Article

    Ai et al Biochemistry. 2007 Apr 20. ():.

    Insert

    mKalama1
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    The fluorescent protein was inserted between XhoI…

    Promoter

    Available since

    May 15, 2007

    Availability

    Academic Institutions and Nonprofits only

18. Hs.KRAS4b G13D

    Plasmid

    #83133

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G13D mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    G13D

    Promoter

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEN_TmiRc3

    Plasmid

    #25748

    Purpose

    Entry vector for cloning miR30-based shRNA driven by TRE promoter.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. Hs.KRAS4b G12D

    Plasmid

    #83131

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12D mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    G12D

    Promoter

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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