Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 5,091 results for: codon optimized

Showing: 1681 - 1700 of 5091 results

  1. ACACA

    Plasmid
    #39083
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ACACA (ACACA Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  4. pAAV-CBh-mKate2-IRES-MCS (C-gap-WT)

    Plasmid
    #105922
    Purpose
    AAV-mediated gene expression from CBh promoter. Contains left AAV2 ITR with an 11bp deletion in C region and canonical right AAV2 ITR
    Depositor
    Marcella Patrick
    Inserts
    mKate2
    IRES-Multiple cloning site
    Use
    AAV
    Tags
    Expression
    Mutation
    codon optimized
    Promoter
    CBh and same CBh promoter as mKate2
    Available since
    Feb. 15, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    105922-plasmid-map-sequence-id-200599

    Enlarge

  5. NXPE1_pcDNA3.4-TOPO

    Plasmid
    #226841
    Purpose
    Expresses NXPE1 protein residues 60-547 in mammalian cells (HEK293T or expi293). x6 His tag and IL2 secretion signal included N terminal with GGGG linker to protein sequence.
    Depositor
    Bert Vogelstein
    Insert
    NXPE1 (NXPE1 Human)
    Use
    Tags
    x6 HIS and an IL2 secretion signal both N termina…
    Expression
    Mammalian
    Mutation
    Codon optimized for expression in human cells
    Promoter
    Available since
    Dec. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    226841-plasmid-map-sequence-id-456206

    Enlarge

  8. pISV-CRISPR/Cas9PGmUbi

    Plasmid
    #209189
    Purpose
    Expression of Cas9 in the model legume Medicago truncatula, Gmubi promoter (PGmubi), DsRed and Basta selection
    Depositor
    Christian Staehelin
    Inserts
    Cas9
    DsRed(deltaB)
    Use
    Tags
    NLS
    Expression
    Plant
    Mutation
    missing BsaI site and plant-codon optimized with …
    Promoter
    CaMV 35S and GmUbi from Glycine max (L.) Merr. cv…
    Available since
    Jan. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    209189-plasmid-map-sequence-id-419145

    Enlarge

  9. SpCas9 iFE

    Plasmid
    #190138
    Purpose
    Expresses SpCas9-POLB (iFE)
    Depositor
    Timothy Lu
    Article
    Nakade et al bioRxiv 2022.12.05.518807
    Insert
    SpCas9-POLB (HiFi iFE)
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Codon Optimized for mammalian cells
    Promoter
    CBh
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    190138-plasmid-map-sequence-id-374863

    Enlarge

  10. pGBW-m4133639

    Plasmid
    #152344
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 3C-like proteinase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;3xFlag
    Expression
    Mammalian
    Mutation
    codon optimized
    Promoter
    CMV
    Available since
    June 17, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152344-plasmid-map-sequence-id-300328

    Enlarge

  11. pIK86

    Plasmid
    #47933
    Purpose
    C. elegans codon-optimized Cas9 with 2xSV40 NLS sequences. It has a T7 promoter for in vitro transcription, germline-compatible 5'UTR, tbb-2 3'UTR and a polyA sequence.
    Depositor
    Iskra Katic
    Article
    Katic et al Genetics. 2013 Aug 26.
    Insert
    Cas9
    Use
    CRISPR and Unspecified
    Tags
    Expression
    Mutation
    Promoter
    T7
    Available since
    Oct. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    47933-plasmid-map-sequence-id-68745

    Enlarge

  12. HPGD

    Plasmid
    #39154
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    HPGD (HPGD Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  15. pHN873

    Plasmid
    #178829
    Purpose
    Bacterial expression for human Saposin A
    Depositor
    Kazuhiro Abe
    Insert
    artificial gene encoding human Saposin A (AR Human)
    Use
    Tags
    MBP, His8, TEV
    Expression
    Bacterial
    Mutation
    Codon-usage was optimized for bacterial expressio…
    Promoter
    T7
    Available since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    178829-plasmid-map-sequence-id-351932

    Enlarge

  16. pKG113

    Plasmid
    #63073
    Purpose
    Retroviral expression of EGFP-TEV-S-tag CENP-T for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    CENP-T (CENPT Human)
    Use
    Retroviral
    Tags
    EGFP and TEV-S
    Expression
    Mammalian
    Mutation
    E. coli codon optimized. G217R mutation compared …
    Promoter
    Available since
    Jan. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63073-plasmid-map-sequence-id-134725

    Enlarge

  20. pGBW-m4133568

    Plasmid
    #152340
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 3C-like proteinase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;V5
    Expression
    Mammalian
    Mutation
    codon optimized
    Promoter
    CMV
    Available since
    July 30, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152340-plasmid-map-sequence-id-420347

    Enlarge

Showing: 1681 - 1700 of 5091 results