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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3,093 results for: try

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Showing: 1761 - 1780 of 3093 results

  1. HTR2C-DuET

    Plasmid
    #213323
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Publication
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    HTR2C (HTR2C Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213323-plasmid-map-sequence-id-430859

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  8. pYPQ289-RVR

    Plasmid
    #138127
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized ErCas12a with D513R, K519V and N523R mutations, without promoter
    Depositor
    Yiping Qi
    Publication
    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:
    Insert
    ErCas12a RVR variant
    Use
    CRISPR; Gateway compatible cas12a entry clone
    Tags
    NLS
    Expression
    Plant
    Mutation
    D513R, K519V and N523R
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    138127-plasmid-map-sequence-id-272088

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  9. pLV-hUV6-sgRNA-dCas9-KRAB-TagBFP2 (identifier AAAA-0244)

    Plasmid
    #202553
    Purpose
    Nontargeting control vector with TagBFP2
    Depositor
    Frederic Meunier
    Publication
    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:
    Insert
    Humanized dCas9-KRAB-TagBFP2 (tag blue fluorescent protein 2) (TRIM28 S. Pyogenes (dCas9), H. sapiens (KRAB), Entacmaea quadricolor (TagBFP2))
    Use
    CRISPR and Lentiviral
    Tags
    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2
    Mutation
    Puromycin-resistance gene in the pLV hU6-sgRNA hU…
    Available since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    202553-plasmid-map-sequence-id-404692

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  10. PP-PMM

    Plasmid
    #25202
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Tb10.70.0370:M1-K245
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids M1-K245.
    Available since
    Aug. 12, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  15. pYPQ265E6

    Plasmid
    #213469
    Purpose
    Gateway entry clone (attL1 & attR5) TadA-CDa-32aa linker-zSpCas9-D10A-2xUGI for C-T base editing
    Depositor
    Yiping Qi
    Publication
    Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:
    Insert
    TadA-CDa-32aa linker-zSpCas9-D10A-2xUGI
    Use
    CRISPR; Gateway compatible tada-cda-32aa linker-z…
    Tags
    No
    Expression
    Plant
    Available since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    213469-plasmid-map-sequence-id-426729

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  18. pYPQ230-E795L

    Plasmid
    #176890
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing LbCas12a with E795L mutation, without promoter
    Depositor
    Yiping Qi
    Publication
    Zhang et al Front Genome Ed. 2022 Jan 31;4:780238. d
    Insert
    LbCas12a-E795L
    Use
    CRISPR; Gateway compatible lbcpf1 entry clone
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Plant
    Available since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    176890-plasmid-map-sequence-id-354003

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  19. KG#901

    Plasmid
    #110936
    Purpose
    Expresses Emerald-RAB-3 in the cholinergic motor neuron DA9 for imaging synaptic vesicles in a single neuron in a living animal
    Depositor
    Kenneth Miller
    Publication
    Edwards et al Genetics. 2018 Nov;210(3):947-968. doi:
    Inserts
    mig-13 promoter
    Emerald-RAB-3
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    Expression
    Bacterial
    Available since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    110936-plasmid-map-sequence-id-216868

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Showing: 1761 - 1780 of 3093 results