We narrowed to 152,541 results for: des

1. pDEST-mCherry-GJA1-20k-N1

   Plasmid

   #49861

   Purpose

   Encodes C-terminal fragment of human connexin 43 initiating at M213 with a V5 C-terminal fusion tag

   Depositor

   Robin Shaw

   Article

   Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

   Insert

   GJA1-20k (GJA1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   GJA1-20k corresponds to residues 213 to 382 to Co…

   Promoter

   CMV

   Available since

   Dec. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA 3.2/V5-DEST RBM14-V5

   Plasmid

   #69823

   Purpose

   Expresses RBM14-V5 in mammalian cells

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   RNA-binding protein 14 (RBM14 Human)
   

   Use

   Retroviral

   Tags

   v5

   Expression

   Mammalian

   Mutation

   Promoter

   cmv

   Available since

   April 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti X2 Puro DEST (w16-1)

   Plasmid

   #17296

   Purpose

   3rd gen lentiviral Gateway destination vector, RNAi, 3’LTR insertion, Puro

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   Lentiviral and RNAi; Destination vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. HA-BirA*-pDEST-N-pcDNA5/FRT/TO

   Plasmid

   #118375

   Purpose

   Expression vector for BioID in mammalian system. Gateway compatible destination vector to generate stable, inducible cell line expressing N-terminally tagged HABirA* and protein of interest.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Type

   Empty backbone

   Use

   Destination

   Tags

   HA-BirA*

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDest-663 (attR4-attR2)

   Plasmid

   #161884

   Purpose

   Multisite Gateway (attR4-attR2) vector for lentivirus production

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFRT/TO/FLAG/HA-DEST DICER

   Plasmid

   #19881

   Depositor

   Thomas Tuschl

   Article

   Landthaler et al RNA. 2008 Oct 31. ():.

   Insert

   Dicer 1, ribonuclease type III (DICER1 Human)
   

   Use

   Tags

   FLAG/HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST-FRT/T0-Flag-BRCA1

   Plasmid

   #71117

   Purpose

   mammalian expression of Flag-BRCA1 siRNA resistant construct

   Depositor

   Daniel Durocher

   Article

   Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

   Insert

   BRCA1 (BRCA1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TO

   Available since

   Nov. 12, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pFRT/TO/FLAG/HA-DEST TNRC6A

   Plasmid

   #19883

   Depositor

   Thomas Tuschl

   Article

   Landthaler et al RNA. 2008 Oct 31. ():.

   Insert

   Trinucleotide repeat containing 6A (TNRC6A Human)
   

   Use

   Tags

   FLAG/HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFRT/TO/FLAG/HA-DEST TNRC6B

   Plasmid

   #19884

   Depositor

   Thomas Tuschl

   Article

   Landthaler et al RNA. 2008 Oct 31. ():.

   Insert

   trinucleotide repeat containing 6B (TNRC6B Human)
   

   Use

   Tags

   FLAG/HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEST17-ScChd1(118-1274)

    Plasmid

    #221207

    Purpose

    ScChd1-aa118-1274 chromatin remodeler

    Depositor

    Gregory Bowman

    Article

    Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

    Insert

    ScChd1 (aa118-1274)
    

    Use

    Tags

    6xHis-tag (Precission protease cleavable)

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-117 and 1275-1468

    Promoter

    Available since

    Aug. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-T7-TALEN(Sangamo)-Destination

    Plasmid

    #37184

    Purpose

    Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses homodimeric FokI domains.

    Depositor

    Pawel Pelczar

    Article

    Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791

    Type

    Empty backbone

    Use

    Tags

    FokI nuclease

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST-DHFR F[1,2]-N (TRP1)

    Plasmid

    #177797

    Purpose

    Gateway destination vector to insert genes of interest having a N-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

    Type

    Empty backbone

    Use

    Tags

    DHFR F[1,2]

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. 370 pTol2-lmo2:CherryDest

    Plasmid

    #73489

    Purpose

    Tol2 vector with lmo2 promoter upstream of Cherry, attR1/R2 cassette, and late SV40 pA signal

    Depositor

    Nathan Lawson

    Article

    Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

    Insert

    lmo2 promoter:mCherry-attR1-attR2
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDEST40-2XFL-Src-S4-S32-KD

    Plasmid

    #140299

    Purpose

    Expresses Flag-tagged human Src-S4-S32-KD in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src deletion mutant (SRC Human)
    

    Use

    Tags

    2X Flag

    Expression

    Mammalian

    Mutation

    lacks the UD region

    Promoter

    CMV

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDEST40-2XFL-Src-S32-KD

    Plasmid

    #140311

    Purpose

    Expresses Flag-tagged human Src-S32-KD in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src deletion mutant (SRC Human)
    

    Use

    Tags

    2X Flag

    Expression

    Mammalian

    Mutation

    Contains only the SH3, SH2, and KD regions

    Promoter

    CMV

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFRT/TO/FLAG/HA-DEST TNRC6C

    Plasmid

    #19885

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Oct 31. ():.

    Insert

    Trinucleotide repeat containing 6C (TNRC6C Human)
    

    Use

    Tags

    FLAG/HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDEST-FRT/TO-3xFLAG-REV7

    Plasmid

    #114127

    Purpose

    Expresses N-terminally tagged 3x FLAG-REV7 in mammalian cells

    Depositor

    Daniel Durocher

    Article

    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

    Insert

    REV7 (MAD2L2 Human)
    

    Use

    Genomic integration, tetracycline inducible

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TetO2

    Available since

    Aug. 16, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pMuLE Lenti Dest eGFP

    Plasmid

    #62175

    Purpose

    MuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses EGFP under the control of a PGK promoter. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Type

    Empty backbone

    Use

    Lentiviral; Mule gateway destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDEST40-2XHA-Src-K298M

    Plasmid

    #140314

    Purpose

    Expresses HA-tagged human Src-K298M in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src mutant (SRC Human)
    

    Use

    Tags

    2X HA

    Expression

    Mammalian

    Mutation

    K298M

    Promoter

    CMV

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCK033 p5E desma MCS

    Plasmid

    #195948

    Purpose

    Gateway p5E vector

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    desmin a regulatory elements (desma Zebrafish)
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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