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Showing: 911 - 920 of 8664 results

  1. pJK627

    Plasmid
    #72462
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Asp/Tyr154>Phe mutant (AaPurE1-S57D,Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutation changes serine-57 to aspartate, tyrosine…
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    72462-plasmid-map-sequence-id-133901

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  2. pJK628

    Plasmid
    #72463
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Ala/Tyr154>Phe mutant (AaPurE1-S57A,Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutation changes serine-57 to alanine, tyrosine-1…
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    72463-plasmid-map-sequence-id-133902

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  3. pJK573

    Plasmid
    #72456
    Purpose
    Produces Yersinia pestis N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal TEV protease-cleavable His6 tag (H6tYpPurE1), synthetic gene
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Mutation
    synthetic gene with ~57 silent substitutions
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    72456-plasmid-map-sequence-id-133910

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  4. pJK632

    Plasmid
    #72465
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Val/Tyr154>Phe mutant (AaPurE1-S57V,Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutation changes serine-57 to valine, tyrosine-15…
    Promoter
    T7
    Available since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    72465-plasmid-map-sequence-id-133904

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  5. pJK629

    Plasmid
    #72464
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Thr/Tyr154>Phe mutant (AaPurE1-S57T,Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutation changes serine-57 to threonine, tyrosine…
    Promoter
    T7
    Available since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    72464-plasmid-map-sequence-id-133903

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  6. pJK541

    Plasmid
    #72447
    Purpose
    Produces Acetobacter aceti 1023 small hypothetical protein (AaOrfX); N5-carboxyaminoimidazole ribonucleotide mutase (AaPurE1); NCAIR synthetase (AaPurK)
    Depositor
    T. J. Kappock
    Inserts
    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7 and n/a
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72447-plasmid-map-sequence-id-133869

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  7. pJK569

    Plasmid
    #72455
    Purpose
    Produces Franciscella tularensis N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal TEV protease-cleavable His6 tag (H6tFtPurE1), synthetic gene
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Mutation
    synthetic gene with ~51 silent substitutions
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72455-plasmid-map-sequence-id-133906

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  8. pJK587

    Plasmid
    #71696
    Purpose
    Produces Acetobacter aceti 1023 thioredoxin reductase 1 with a C-terminal His6 tag and Cys138>Ser mutant (AaTrxB1H6-C138S)
    Depositor
    T. J. Kappock
    Insert
    thioredoxin reductase 1
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes cysteine-138 to serine
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71696-plasmid-map-sequence-id-131733

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  9. pJK525

    Plasmid
    #71601
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Trp97>Tyr mutant (AaPurE1fyw)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, tryptopha…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71601-plasmid-map-sequence-id-131675

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  10. pJK526

    Plasmid
    #71602
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp97>Tyr/Trp165>Phe mutant (AaPurE1wyf)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-97 to tyrosine, tryptophan-165…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71602-plasmid-map-sequence-id-131676

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Showing: 911 - 920 of 8664 results