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Addgene

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We narrowed to 43,126 results for: cha

Showing: 3641 - 3680 of 43126 results

  5. pDual

    Plasmid
    #207883
    Purpose
    Expresses thermosensitive variants of the homing endonucleases I-OnuI and I-GpeMI, each in a different open reading frame. Both were made thermosensitive by a fusion with the L212P VMA1 intein.
    Depositor
    David Edgell
    Article
    Foo et al Nucleic Acids Res. 2024 Feb 28;52(4):206
    Inserts
    Thermosensitive fusion of I-OnuI and the L212P VMA1 intein.
    Thermosensitive fusion of I-GpeMI and the L212P VMA1 intein.
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Leucine 212 changed to proline, leucine 434 in th…
    Promoter
    Available since
    Aug. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    207883-plasmid-map-sequence-id-422555

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  6. Cav2.2 HA EIVA (E1658A) pCAGGS

    Plasmid
    #206082
    Purpose
    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation in the P loop of Domain IV which reduces trafficking to the cell surface
    Depositor
    Annette Dolphin
    Article
    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi
    Insert
    cacna1b (CACNA1B Rabbit)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    E1658A
    Promoter
    CMV/B-actin
    Available since
    Oct. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    206082-plasmid-map-sequence-id-411174

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  7. Cav2.2 HA EIA (E314A) pCAGGS

    Plasmid
    #206081
    Purpose
    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation in the P loop of Domain I which reduces trafficking to the cell surface
    Depositor
    Annette Dolphin
    Article
    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi
    Insert
    cacna1b (CACNA1B Rabbit)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    E314A
    Promoter
    CMV/B-actin
    Available since
    Oct. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    206081-plasmid-map-sequence-id-411173

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  8. Cav2.2 HA EIVK (E1658K) pcDNA3

    Plasmid
    #206066
    Purpose
    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658K mutation in the P loop of Domain IV which abolishes trafficking to the cell surface
    Depositor
    Annette Dolphin
    Article
    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi
    Insert
    cacna1b (CACNA1B Rabbit)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    E1658K
    Promoter
    CMV
    Available since
    Oct. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    206066-plasmid-map-sequence-id-410269

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  9. Cav2.2 HA EIVK (E1658K) pCAGGS

    Plasmid
    #206096
    Purpose
    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658K mutation in the P loop of Domain IV which abolishes trafficking to the cell surface
    Depositor
    Annette Dolphin
    Article
    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi
    Insert
    cacna1b (CACNA1B Rabbit)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    E1658K
    Promoter
    CMV/B-actin
    Available since
    Sept. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    206096-plasmid-map-sequence-id-406757

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  12. pLgw EcoDam-V5-NKX2-5Y191C

    Plasmid
    #98598
    Purpose
    Mammalian DamID lentiviral vector for fusing NKX2-5 Y191C with Dam-V5 using Gateway cloning
    Depositor
    Richard Harvey
    Article
    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.
    Insert
    NKX2-5 (Nkx2-5 Mouse)
    Use
    Lentiviral; Damid
    Tags
    Dam (DNA adenine methyltransferase) and V5
    Expression
    Mammalian
    Mutation
    Y191C point mutant
    Promoter
    Heat Shock Minimal Promoter
    Available since
    Aug. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    98598-plasmid-map-sequence-id-190106

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  13. pLgw NKX2-5Y191C-V5-EcoDam

    Plasmid
    #98599
    Purpose
    Mammalian DamID lentiviral vector for fusing NKX2-5 Y191C with Dam-V5 using Gateway cloning
    Depositor
    Richard Harvey
    Article
    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.
    Insert
    NKX2-5 (Nkx2-5 Mouse)
    Use
    Lentiviral; Damid
    Tags
    V5 and Dam (DNA adenine methyltransferase)
    Expression
    Mammalian
    Mutation
    Y191C point mutant
    Promoter
    Heat Shock Minimal Promoter
    Available since
    July 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    98599-plasmid-map-sequence-id-189181

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  24. Cav2.1 HA EI,II,III,IVA (E320A, E670A, E1411A, E1707A) pcDNA3

    Plasmid
    #206121
    Purpose
    expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domains I, II, III and IV to to abolish divalent cation binding
    Depositor
    Annette Dolphin
    Article
    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi
    Insert
    cacna1a (Cacna1a Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    E320A, E670A, E1411A, E1707A
    Promoter
    CMV
    Available since
    Oct. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    206121-plasmid-map-sequence-id-410287

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  35. GFP Cav2.2 HA EI,II,III,IVA (E314A, E664A, E1370A, E1658A) pcDNA3

    Plasmid
    #206093
    Purpose
    Cav2.2 calcium channel with a N-terminal GFP tag, exofacial double HA tag in domain II and mutations in the P loop of Domains I, II, III and IV which abolish trafficking to the cell surface
    Depositor
    Annette Dolphin
    Article
    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi
    Insert
    cacna1b (CACNA1B Rabbit)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    E314A, E664A, E1370A, E1658A
    Promoter
    CMV
    Available since
    Nov. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    206093-plasmid-map-sequence-id-415677

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  36. Cav2.2 HA EI,II,III,IVA (E314A, E664A, E1370A, E1658A) pCAGGS

    Plasmid
    #206098
    Purpose
    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and mutations in the P loop of Domains I, II, III and IV which abolish trafficking to the cell surface
    Depositor
    Annette Dolphin
    Article
    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi
    Insert
    cacna1b (CACNA1B Rabbit)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    E314A, E664A, E1370A, E1658A
    Promoter
    CMV/B-actin
    Available since
    Nov. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    206098-plasmid-map-sequence-id-415670

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Showing: 3641 - 3680 of 43126 results