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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 1841 - 1860 of 7951 results

  2. gCOTS-pyl

    Plasmid
    #92048
    Purpose
    This is a S. elongatus (PCC7942) shuttle vector used for recombination of the pylRS orthogonal translation system to genomic neutral site 2.
    Depositor
    Lital Alfonta
    Article
    Chemla et al Biochemistry. 2017 Apr 12. doi: 10.1021/
    Inserts
    Pyrrolysyl tRNA(cua)
    Pyrrolysyl tRNA synthetase (methanosarcina mazei)
    Use
    Cyanobacteria s. elongatus genome recombination i…
    Promoter
    LeuP (native S. elongatus promoter) and PrcbL
    Available Since
    Aug. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    92048-plasmid-map-sequence-id-181707

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  5. pBad-CA TAG20

    Plasmid
    #105666
    Purpose
    expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 20 and C-terminal 6x his tag
    Depositor
    Ryan Mehl
    Article
    Mehl et al 2020
    Insert
    human carbonic anhydrase II thermostable variant (CA2 Human)
    Tags
    6x his tag
    Expression
    Bacterial
    Mutation
    TAG stop codon at site 20
    Promoter
    pBad/arabinose
    Available Since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    105666-plasmid-map-sequence-id-205513

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  6. pBad-CA TAG93

    Plasmid
    #105667
    Purpose
    expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 93 and C-terminal 6x his tag
    Depositor
    Ryan Mehl
    Article
    Mehl et al 2020
    Insert
    human carbonic anhydrase II thermostable variant (CA2 Human)
    Tags
    6x his tag
    Expression
    Bacterial
    Mutation
    TAG stop codon at site 93
    Promoter
    pBad/arabinose
    Available Since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    105667-plasmid-map-sequence-id-205514

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  8. pBad-CA TAG233

    Plasmid
    #105838
    Purpose
    Expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 233 and C-terminal 6x his tag
    Depositor
    Ryan Mehl
    Article
    Mehl et al 2020
    Insert
    human carbonic anhydrase II thermostable variant (CA2 Human)
    Tags
    6x his tag
    Expression
    Bacterial
    Mutation
    TAG stop codon at site 233
    Promoter
    pBad/arabinose
    Available Since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    105838-plasmid-map-sequence-id-205520

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  9. pBad-CAts TAG97

    Plasmid
    #105668
    Purpose
    expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 97 and C-terminal 6x his tag
    Depositor
    Ryan Mehl
    Article
    Mehl et al 2020
    Insert
    human carbonic anhydrase II thermostable variant (CA2 Human)
    Tags
    6x his tag
    Expression
    Bacterial
    Mutation
    TAG stop codon at site 97
    Promoter
    pBad/arabinose
    Available Since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    105668-plasmid-map-sequence-id-205515

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  17. pYPQ166

    Plasmid
    #109328
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS without promoter
    Depositor
    Yiping Qi
    Article
    Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.
    Insert
    zCas9
    Use
    CRISPR; Gateway compatible zcas9 entry clone
    Tags
    3x FLAG, NLS and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Available Since
    June 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    109328-plasmid-map-sequence-id-210215

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Showing: 1841 - 1860 of 7951 results