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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,136 results for: eng

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Showing: 1881 - 1900 of 14136 results

  1. pACB111

    Plasmid
    #222210
    Purpose
    For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the G76V mutation and a C-terminal His tag
    Depositor
    Gregg Beckham
    Article
    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.
    Insert
    fghA(G76V)
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Changed glycine 76 to valine
    Promoter
    T7
    Available Since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222210-plasmid-map-sequence-id-437376

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  2. pACB112

    Plasmid
    #222211
    Purpose
    For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the S11R mutation and a C-terminal His tag
    Depositor
    Gregg Beckham
    Article
    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.
    Insert
    fghA(S11R)
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Changed Serine 11 to Arginine
    Promoter
    T7
    Available Since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222211-plasmid-map-sequence-id-437378

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  3. pACB113

    Plasmid
    #222212
    Purpose
    For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the W15C mutation and a C-terminal His tag
    Depositor
    Gregg Beckham
    Article
    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.
    Insert
    fghA(W15C)
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Changed Tryptophan 15 to Cysteine
    Promoter
    T7
    Available Since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222212-plasmid-map-sequence-id-437381

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  4. pTC3cIfm

    Plasmid
    #193778
    Purpose
    Expresses frame-shifted cI under the control of PLTetO-1 promoter, p15A origin of replication, Chloramphenicol selection
    Depositor
    Sangram Bagh
    Article
    Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11
    Insert
    Frame-shifted CI
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    PLTetO-1
    Available Since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    193778-plasmid-map-sequence-id-389809

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  5. pTS1043

    Plasmid
    #169621
    Purpose
    Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PPGK-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::PPGK-IgkSS-Nluc-p2A-mTagBFP2-pA) .
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PCMV-driven expression of SEAP and iRFP and PPGK-driven expression of secreted Nluc and mTagBFP2
    Expression
    Mammalian
    Promoter
    PhCMV / PPGK
    Available Since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    169621-plasmid-map-sequence-id-366769

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  6. pTS2333_Tier3(Lenti_inverse)-YPet_Puro

    Plasmid
    #169664
    Purpose
    Tier-3 vector for stable integration via lentiviral transduction in inverted direction with polyA carrying a constitutive expression cassette for puromyine and Ypet (PCMV-5'LTR-Psi-gag-env-PRPBSA-YPet-p2A-PuroR-pA::A2-pA::A3-pA-WPRE-3'LTR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PRPBSA-driven YPet and PuroR expression
    Expression
    Mammalian
    Promoter
    PhCMV / RPBSA
    Available Since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    169664-plasmid-map-sequence-id-365639

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  7. pTS2361_Tier3(SB)-rtTA-YPet_PuroR

    Plasmid
    #169657
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a YPet marker and PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
    Expression
    Mammalian
    Promoter
    PRPBSA
    Available Since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    169657-plasmid-map-sequence-id-365632

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  8. pTS1042

    Plasmid
    #169620
    Purpose
    Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (PhCMV-SEAP-p2A-iRFP670-pA::PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PCMV-driven expression of SEAP and iRFP and PPGK-driven expression of secreted Nluc and mTagBFP2
    Expression
    Mammalian
    Promoter
    PhCMV / PPGK
    Available Since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    169620-plasmid-map-sequence-id-365622

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  9. pTS2363_Tier3(PB)-rtTA-YPet_PuroR

    Plasmid
    #169659
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a YPet marker and PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
    Expression
    Mammalian
    Promoter
    PRPBSA
    Available Since
    June 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    169659-plasmid-map-sequence-id-364557

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Showing: 1881 - 1900 of 14136 results