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POC1428

Plasmid

#221575

Purpose

Plasmid containing the Insert 3 to be assembled in POC1430 using the site-selective methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by methylation-protectable BsaI sites and containing one always-methylable internal BsaI site

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1429

Plasmid

#221576

Purpose

Plasmid containing the Insert 4 to be assembled in POC1430 using the site-selective methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by methylation-protectable BsaI sites and containing one always-methylable internal BsaI site

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKrev-CGI

Plasmid

#225568

Purpose

packaging plasmid for lentivector production

Depositor

Ferdinando Pucci

Article

Ziglari et al Cancer Res. 2025 Mar 3;85(5):859-874. do

Type

Empty backbone

Expression

Mammalian

Available Since

March 12, 2025

Availability

Academic Institutions and Nonprofits only

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p48.unipolyA.dNGFR.mCMV.PGK.eGFP.Gem1.WPRE

Plasmid

#226767

Purpose

vector with NGFR and GFP

Depositor

Ferdinando Pucci

Article

Ziglari et al Cancer Res. 2025 Mar 3;85(5):859-874. do

Type

Empty backbone

Expression

Mammalian

Available Since

March 12, 2025

Availability

Academic Institutions and Nonprofits only

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p49.unipolyA.Cdt1.mSca.mCMV.PGK.eGFP.Gem1.WPRE

Plasmid

#226768

Purpose

vector with mScarlet fluorescent protein and GFP

Depositor

Ferdinando Pucci

Article

Ziglari et al Cancer Res. 2025 Mar 3;85(5):859-874. do

Type

Empty backbone

Expression

Mammalian

Available Since

March 12, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1486

Plasmid

#213811

Purpose

BpiI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

BpiI-associated non-switchable methylase M2.HpyAII

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pJD1

Plasmid

#222374

Purpose

Recombinant human IgG1 antibody (1F8) that binds to Tasmanian devil PDL1

Depositor

Andrew S. Flies

Article

Slyp et al Methods Mol Biol. 2024;2826:231-249. doi

Insert

IGHG

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pAF125

Plasmid

#222373

Purpose

Recombinant human IgG antibody vector without variable regions. Contains mTagBFP marker.

Depositor

Andrew S. Flies

Article

Slyp et al Methods Mol Biol. 2024;2826:231-249. doi

Insert

IGHG (partial)

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1464

Plasmid

#213736

Purpose

BsaI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

Non-switchable methylase M2.Eco31I

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1481

Plasmid

#213807

Purpose

LguI-associated switch methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

LguI-associated switch methylase M.XmnI

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1474

Plasmid

#213762

Purpose

BpiI-associated switch methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

BpiI-associated switch methylase M2.NmeMC58II

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1488

Plasmid

#213813

Purpose

BpiI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

BpiI-associated non-switchable methylase M1.MboII

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1479

Plasmid

#213805

Purpose

BsaI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

Non-switchable methylase M1.Eco31I

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1480

Plasmid

#213806

Purpose

BsaI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

Non-switchable methylase M1.Eco31I

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1484

Plasmid

#213810

Purpose

BpiI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

BpiI-associated non-switchable methylase M1.HpyAII

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1466

Plasmid

#213754

Purpose

BsaI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

Truncated non-switchable methylase M2.Eco31I

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1482

Plasmid

#213808

Purpose

LguI-associated switch methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

LguI-associated switch methylase M.XmnI

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1483

Plasmid

#213809

Purpose

BpiI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

BpiI-associated non-switchable methylase M1.HpyAII

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1472

Plasmid

#213760

Purpose

BsaI-associated switch methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

BsaI-associated switch methylase M.Osp807II

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1470

Plasmid

#213758

Purpose

BsaI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

Truncated non-switchable methylase M2.BsaI

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge