Addgene

We narrowed to 5,527 results for: PEP

Showing: 1921 - 1940 of 5527 results

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EC.SurA.pTYB1.a21-428.L239R

Plasmid

#15957

Depositor

David McKay

Article

Xu et al J Mol Biol. 2007 Oct 19. 373(2):367-81.

Insert

surA (surA E. coli)

Use

Tags

intein

Expression

Bacterial

Mutation

L239R

Promoter

Available since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only
pJC45BS104 L892W

Plasmid

#1308

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 L892W (HSP104 Budding Yeast)

Use

Tags

10X His tag

Expression

Bacterial

Mutation

Changed Leu 892 to Trp

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pJC45BS104 F130W

Plasmid

#1302

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 F130W (HSP104 Budding Yeast)

Use

Tags

10X His tag

Expression

Bacterial

Mutation

Changed Phe 130 to Trp

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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EC.SurA.pTYB1.a21-428.M231R

Plasmid

#15956

Depositor

David McKay

Article

Xu et al J Mol Biol. 2007 Oct 19. 373(2):367-81.

Insert

mature SurA protein M231R mutant

Use

Tags

intein

Expression

Bacterial

Mutation

M231R

Promoter

Available since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only
pJC45BS104 L553W

Plasmid

#1306

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 L553W (HSP104 Budding Yeast)

Use

Tags

10X His tag

Expression

Bacterial

Mutation

Changed Leu 553 to Trp

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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BtA2

Plasmid

#238982

Purpose

In vitro transcription of bovine arrestin-2 long isoform

Depositor

Jeffrey Benovic, Vsevolod Gurevich

Article

Sterne-Marr et al J Biol Chem. 1993 Jul 25;268(21):15640-8

Insert

arrestin-2 long (ARRB2 Bovine)

Use

In vitro transcription

Tags

Expression

Mutation

none

Promoter

SP6

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-mCherry-CW3SL

Plasmid

#223662

Purpose

Expresses mCherry in a Cre-dependent manner

Depositor

Oscar Marin

Article

Selten et al Nature. 2025 Apr 30. doi: 10.1038/s41586

Insert

mCherry

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-fDIO-hM3D(Gq)-mCherry

Plasmid

#223652

Purpose

Expresses hM3D(Gq) in a flippase dependent manner

Depositor

Oscar Marin

Article

Selten et al Nature. 2025 Apr 30. doi: 10.1038/s41586

Insert

hM3D(Gq)-mCherry

Use

AAV

Tags

mCherry

Expression

Mutation

Promoter

hSyn

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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POSV801-SP44-SnaAinBCDEOT1T2

Plasmid

#225056

Purpose

Heterologous expression in Streptomyces with inactive SnaA

Depositor

Wilfred van der Donk

Article

Yu et al ACS Cent Sci. 2024 Jun 3;10(6):1242-1250

Insert

inactivated SnaA

Use

Tags

Expression

Bacterial

Mutation

S556A/S1616A

Promoter

SP44

Available since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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POSV801-SP44-SnaABCDEinOT1T2

Plasmid

#225057

Purpose

Heterologous expression in Streptomyces with inactive SnaE

Depositor

Wilfred van der Donk

Article

Yu et al ACS Cent Sci. 2024 Jun 3;10(6):1242-1250

Insert

inactivated SnaE

Use

Tags

Expression

Bacterial

Mutation

deltaA46-V801

Promoter

SP44

Available since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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pGH1110

Plasmid

#223682

Purpose

U6-CloningSite-Ef1a-Cas9-NLS-Flag-T2A-EGFP-P2A-BlastR

Depositor

Gaelen Hess

Article

Liila-Fogarty et al ACS Synth Biol. 2024 Oct 19. doi: 10.102

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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POSV801-SP44-SnaABCDEOinT1T2

Plasmid

#225058

Purpose

Heterologous expression in Streptomyces with inactive SnaO

Depositor

Wilfred van der Donk

Article

Yu et al ACS Cent Sci. 2024 Jun 3;10(6):1242-1250

Insert

inactivated SnaO

Use

Tags

Expression

Bacterial

Mutation

deltaR51-V511

Promoter

SP44

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pGGD_MUTT2A_E

Plasmid

#218899

Purpose

golden gate cloning

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

MUTT2A

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pGWB602-po-mCerulean

Plasmid

#213861

Purpose

Binary vector for the expression of peroxisome transit peptide fused mCerulean in plants (for Agrobacterium-mediated genetic transformation and visualization of peroxisome.)

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant Cell Rep. 2023 Mar;42(3):599-607.

Insert

Peroxisome transit peptide fused mCerulean

Use

Tags

Expression

Bacterial and Plant

Mutation

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA TfR1-PuRRRE

Plasmid

#208327

Purpose

C-terminal PuRRRE-tagged transferrin receptor 1 (tag on extracellular domain).

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TfR1-PuRRRE

Use

Tags

PuRRRE

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA TfR1-TREER

Plasmid

#208325

Purpose

C-terminal TREER-tagged transferrin receptor 1 (tag on extracellular domain).

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TfR1-TREER

Use

Tags

TREER

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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Tc-INTR-GFP

Plasmid

#208899

Purpose

encodes the inotocin receptor (oxytocin/vasopressin-like) from the beetle Tribolium castaneum, tagged with GFP

Depositor

Christian Gruber

Article

Keov et al Sci Rep. 2018 Jul 3;8(1):10020. doi: 10.

Insert

INTR

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pGWT35S-TP-UnaG

Plasmid

#203484

Purpose

For particle bombardment. A plastid transit peptide fused UnaG under the control of the cauliflower mosaic virus (CaMV) 35S promoter.

Depositor

Yutaka Kodama

Article

Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

Insert

Plastid transit peptide fused UnaG

Use

Tags

Expression

Bacterial and Plant

Mutation

Promoter

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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1BR9

Plasmid

#193862

Purpose

E. coli expression vector for N-ter GFP11 and C-ter R9 tagged proteins. Empty backbone for LIC cloning.

Depositor

Markita Landry

Article

Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

Type

Empty backbone

Use

Tags

GFP11, Hisx6, and R9

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pVRC-N-Venus-V150A-CC-Di

Plasmid

#190707

Purpose

Encodes a modified version (V150A) of N-Venus followed by the parallel coiled-coil CC-Di.

Depositor

Nigel Savery

Article

Rhys et al Nat Chem Biol. 2022 Jul 14. pii: 10.1038

Insert

N-Venus-V150A-CC-Di

Use

Tags

Expression

Mutation

V150A

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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